LOCUS       sequence-87400-e        2104 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Cytokeratin, Excitation: 493, Emission: 509.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2104)
  TITLE     Davidson Green fluorescent proteins
REFERENCE   2  (bases 1 to 2104)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: mWasabi-Keratin-17
FEATURES             Location/Qualifiers
     source          1..2104
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      1357..1366
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             1363..2073
                     /codon_start=1
                     /product="green variant of teal fluorescent protein mTFP1 
                     (Ai et al., 2008)"
                     /label=mWasabi
                     /translation="MVSKGEETTMGVIKPDMKIKLKMEGNVNGHAFVIEGEGEGKPYDG
                     TNTINLEVKEGAPLPFSYDILTTAFSYGNRAFTKYPDDIPNYFKQSFPEGYSWERTMTF
                     EDKGIVKVKSDISMEEDSFIYEIHLKGENFPPNGPVMQKETTGWDASTERMYVRDGVLK
                     GDVKMKLLLEGGGHHRVDFKTIYRAKKAVKLPDYHFVDHRIEILNHDKDYNKVTVYEIA
                     VARNSTDGMDELYK"
ORIGIN
        1 gacgtcagat ccgctagcac catgagcttc accactcgct ccaccttctc caccaactac
       61 cggtccctgg gctctgtcca ggcgcccagc tacggcgccc ggccggtcag cagcgcggcc
      121 agcgtctatg caggcgctgg gggctctggt tcccggatct ccgtgtcccg ctccaccagc
      181 ttcaggggcg gcatggggtc cgggggcctg gccaccggga tagccggggg tctggcagga
      241 atgggaggca tccagaacga gaaggagacc atgcaaagcc tgaacgaccg cctggcctct
      301 tacctggaca gagtgaggag cctggagacc gagaaccgga ggctggagag caaaatccgg
      361 gagcacttgg agaagaaggg accccaggtc agagactgga gccattactt caagatcatc
      421 gaggacctga gggctcagat cttcgcaaat actgtggaca atgcccgcat cgttctgcag
      481 attgacaatg cccgtcttgc tgctgatgac tttagagtca agtatgagac agagctggcc
      541 atgcgccagt ctgtggagaa cgacatccat gggctccgca aggtcattga tgacaccaat
      601 atcacacgac tgcagctgga gacagagatc gaggctctca aggaggagct gctcttcatg
      661 aagaagaacc acgaagagga agtaaaaggc ctacaagccc agattgccag ctctgggttg
      721 accgtggagg tagatgcccc caaatctcag gacctcgcca agatcatggc agacatccgg
      781 gcccaatatg acgagctggc tcggaagaac cgagaggagc tagacaagta ctggtctcag
      841 cagattgagg agagcaccac agtgctcacc acacagtctg ctgaggttgg agctgctgag
      901 acgacgctca cagagctgag acgtacagtc cagtccttgg agatcgacct ggactccatg
      961 agaaatctga aggccagctt ggagaacagc ctgagggagg tggaggcccg ctacgcccta
     1021 cagatggagc agctcaacgg gatcctgctg caccttgagt cagagctggc acagacccgg
     1081 gcagagggac agcgccaggc ccaggagtat gaggccctgc tgaacatcaa ggtcaagctg
     1141 gaggctgaga tcgccaccta ccgccgcctg ctggaagatg gcgaggactt taatcttggt
     1201 gatgccttgg acagcagcaa ctccatgcaa accatccaaa agaccaccac ccgccggata
     1261 gtggatggca aagtggtgtc tgagaccaat gacaccaaag ttctgaggca tcgaattctg
     1321 cagtcgacgg taccgcgggc ccgggatcca ccggtcgcca ccatggtgag caagggcgag
     1381 gagaccacaa tgggcgtaat caagcccgac atgaagatca agctgaagat ggagggcaac
     1441 gtgaatggcc acgccttcgt gatcgagggc gagggcgagg gcaagcccta cgacggcacc
     1501 aacaccatca acctggaggt gaaggaggga gcccccctgc ccttctccta cgacattctg
     1561 accaccgcgt tcagttacgg caacagggcc ttcaccaagt accccgacga catccccaac
     1621 tacttcaagc agtccttccc cgagggctac tcttgggagc gcaccatgac cttcgaggac
     1681 aagggcatcg tgaaggtgaa gtccgacatc tccatggagg aggactcctt catctacgag
     1741 atacacctca agggcgagaa cttccccccc aacggccccg tgatgcagaa ggagaccacc
     1801 ggctgggacg cctccaccga gaggatgtac gtgcgcgacg gcgtgctgaa gggcgacgtc
     1861 aagatgaagc tgctgctgga gggcggcggc caccaccgcg ttgacttcaa gaccatctac
     1921 agggccaaga aggcggtgaa gctgcccgac tatcactttg tggaccaccg catcgagatc
     1981 ctgaaccacg acaaggacta caacaaggtg accgtttacg agatcgccgt ggcccgcaac
     2041 tccaccgacg gcatggacga gctgtacaag taagcggccg cgactctaga tcataatcag
     2101 ccat
//
