LOCUS       sequence-87402-e         944 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Mitochondria, Excitation: 493, Emission: 509.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 944)
  AUTHORS   Rizzo MA, Davidson MW, Piston DW
  TITLE     Fluorescent protein tracking and detection: fluorescent protein 
            structure and color variants.
  JOURNAL   Cold Spring Harb Protoc. 2009 Dec;2009(12):pdb.top63. doi: 
            10.1101/pdb.top63.
  PUBMED    20150100
REFERENCE   2  (bases 1 to 944)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cold Spring
            Harb Protoc."; date: "2009-12"; volume: "2009(12):pdb.top63. doi"; 
            pages: " 10.1101/pdb.top63"
COMMENT     Sequence Label: mWasabi-Mito-7
FEATURES             Location/Qualifiers
     source          1..944
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        4..55
                     /label=CMVd2 promoter
                     /note="truncated human cytomegalovirus (CMV) immediate 
                     early promoter that yields moderately reduced expression 
                     relative to the full-length promoter"
     primer_bind     6..26
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     CDS             84..158
                     /codon_start=1
                     /product="mitochondrial presequence of human cytochrome c 
                     oxidase subunit VIII (Rizutto et al., 1989)"
                     /label=COX8 presequence
                     /translation="MSVLTPLLLRGLTGSARRLPVPRAK"
     regulatory      186..195
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             192..902
                     /codon_start=1
                     /product="green variant of teal fluorescent protein mTFP1 
                     (Ai et al., 2008)"
                     /label=mWasabi
                     /translation="MVSKGEETTMGVIKPDMKIKLKMEGNVNGHAFVIEGEGEGKPYDG
                     TNTINLEVKEGAPLPFSYDILTTAFSYGNRAFTKYPDDIPNYFKQSFPEGYSWERTMTF
                     EDKGIVKVKSDISMEEDSFIYEIHLKGENFPPNGPVMQKETTGWDASTERMYVRDGVLK
                     GDVKMKLLLEGGGHHRVDFKTIYRAKKAVKLPDYHFVDHRIEILNHDKDYNKVTVYEIA
                     VARNSTDGMDELYK"
ORIGIN
        1 catgacgcaa atgggcggta ggcgtgtacg gtgggaggtc tatataagca gagctggttt
       61 agtgaaccgt cagatccgct agcatgtccg tcctgacgcc gctgctgctg cggggcttga
      121 caggctcggc ccggcggctc ccagtgccgc gcgccaagat ccattcgttg ggggatccac
      181 cggtcgccac catggtgagc aagggcgagg agaccacaat gggcgtaatc aagcccgaca
      241 tgaagatcaa gctgaagatg gagggcaacg tgaatggcca cgccttcgtg atcgagggcg
      301 agggcgaggg caagccctac gacggcacca acaccatcaa cctggaggtg aaggagggag
      361 cccccctgcc cttctcctac gacattctga ccaccgcgtt cagttacggc aacagggcct
      421 tcaccaagta ccccgacgac atccccaact acttcaagca gtccttcccc gagggctact
      481 cttgggagcg caccatgacc ttcgaggaca agggcatcgt gaaggtgaag tccgacatct
      541 ccatggagga ggactccttc atctacgaga tacacctcaa gggcgagaac ttccccccca
      601 acggccccgt gatgcagaag gagaccaccg gctgggacgc ctccaccgag aggatgtacg
      661 tgcgcgacgg cgtgctgaag ggcgacgtca agatgaagct gctgctggag ggcggcggcc
      721 accaccgcgt tgacttcaag accatctaca gggccaagaa ggcggtgaag ctgcccgact
      781 atcactttgt ggaccaccgc atcgagatcc tgaaccacga caaggactac aacaaggtga
      841 ccgtttacga gatcgccgtg gcccgcaact ccaccgacgg catggacgag ctgtacaagt
      901 aagcggccgc gactctagat cataatcagc cataccacat tgag
//
