LOCUS       sequence-87404-e        2130 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Microtubules, Excitation: 493, Emission: 509.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2130)
  TITLE     Davidson Green fluorescent proteins
REFERENCE   2  (bases 1 to 2130)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: mWasabi-Tubulin-6
FEATURES             Location/Qualifiers
     source          1..2130
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      29..38
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             35..742
                     /codon_start=1
                     /product="green variant of teal fluorescent protein mTFP1 
                     (Ai et al., 2008)"
                     /label=mWasabi
                     /translation="MVSKGEETTMGVIKPDMKIKLKMEGNVNGHAFVIEGEGEGKPYDG
                     TNTINLEVKEGAPLPFSYDILTTAFSYGNRAFTKYPDDIPNYFKQSFPEGYSWERTMTF
                     EDKGIVKVKSDISMEEDSFIYEIHLKGENFPPNGPVMQKETTGWDASTERMYVRDGVLK
                     GDVKMKLLLEGGGHHRVDFKTIYRAKKAVKLPDYHFVDHRIEILNHDKDYNKVTVYEIA
                     VARNSTDGMDELYK"
ORIGIN
        1 gacgtcagat ccgctagcgc taccggtcgc caccatggtg agcaagggcg aggagaccac
       61 aatgggcgta atcaagcccg acatgaagat caagctgaag atggagggca acgtgaatgg
      121 ccacgccttc gtgatcgagg gcgagggcga gggcaagccc tacgacggca ccaacaccat
      181 caacctggag gtgaaggagg gagcccccct gcccttctcc tacgacattc tgaccaccgc
      241 gttcagttac ggcaacaggg ccttcaccaa gtaccccgac gacatcccca actacttcaa
      301 gcagtccttc cccgagggct actcttggga gcgcaccatg accttcgagg acaagggcat
      361 cgtgaaggtg aagtccgaca tctccatgga ggaggactcc ttcatctacg agatacacct
      421 caagggcgag aacttccccc ccaacggccc cgtgatgcag aaggagacca ccggctggga
      481 cgcctccacc gagaggatgt acgtgcgcga cggcgtgctg aagggcgacg tcaagatgaa
      541 gctgctgctg gagggcggcg gccaccaccg cgttgacttc aagaccatct acagggccaa
      601 gaaggcggtg aagctgcccg actatcactt tgtggaccac cgcatcgaga tcctgaacca
      661 cgacaaggac tacaacaagg tgaccgttta cgagatcgcc gtggcccgca actccaccga
      721 cggcatggac gagctgtaca agtccggact cagatctcga gtgcgtgagt gcatctccat
      781 ccacgttggc caggctggtg tccagattgg caatgcctgc tgggagctct actgcctgga
      841 acacggcatc cagcccgatg gccagatgcc aagtgacaag accattgggg gaggagatga
      901 ctccttcaac accttcttca gtgagacggg cgctggcaag cacgtgcccc gggctgtgtt
      961 tgtagacttg gaacccacag tcattgatga agttcgcact ggcacctacc gccagctctt
     1021 ccaccctgag cagctcatca caggcaagga agatgctgcc aataactatg cccgagggca
     1081 ctacaccatt ggcaaggaga tcattgacct tgtgttggac cgaattcgca agctggctga
     1141 ccagtgcacc ggtcttcagg gcttcttggt tttccacagc tttggtgggg gaactggttc
     1201 tgggttcacc tccctgctca tggaacgtct ctcagttgat tatggcaaga agtccaagct
     1261 ggagttctcc atttacccag caccccaggt ttccacagct gtagttgagc cctacaactc
     1321 catcctcacc acccacacca ccctggagca ctctgattgt gccttcatgg tagacaatga
     1381 ggccatctat gacatctgtc gtagaaacct cgatatcgag cgcccaacct acactaacct
     1441 taaccgcctt attagccaga ttgtgtcctc catcactgct tccctgagat ttgatggagc
     1501 cctgaatgtt gacctgacag aattccagac caacctggtg ccctaccccc gcatccactt
     1561 ccctctggcc acatatgccc ctgtcatctc tgctgagaaa gcctaccatg aacagctttc
     1621 tgtagcagag atcaccaatg cttgctttga gccagccaac cagatggtga aatgtgaccc
     1681 tcgccatggt aaatacatgg cttgctgcct gttgtaccgt ggtgacgtgg ttcccaaaga
     1741 tgtcaatgct gccattgcca ccatcaaaac caagcgcagc atccagtttg tggattggtg
     1801 ccccactggc ttcaaggttg gcatcaacta ccagcctccc actgtggtgc ctggtggaga
     1861 cctggccaag gtacagagag ctgtgtgcat gctgagcaac accacagcca ttgctgaggc
     1921 ctgggctcgc ctggaccaca agtttgacct gatgtatgcc aagcgtgcct ttgttcactg
     1981 gtacgtgggt gaggggatgg aggaaggcga gttttcagag gcccgtgaag atatggctgc
     2041 ccttgagaag gattatgagg aggttggtgt ggattctgtt gaaggagagg gtgaggaaga
     2101 aggagaggaa tactaaggat ccaccggatc
//
