LOCUS       sequence-87408-e        1207 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Nucleus/Histones, Excitation: 493, Emission: 505.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1207)
  TITLE     Davidson Green fluorescent proteins
REFERENCE   2  (bases 1 to 1207)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: ZsGreen1-H2B-6
FEATURES             Location/Qualifiers
     source          1..1207
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             78..455
                     /codon_start=1
                     /gene="HIST1H2BJ"
                     /product="human histone H2B"
                     /label=H2B
                     /translation="MPEPAKSAPAPKKGSKKAVTKAQKKGGKKRKRSRKESYSIYVYKV
                     LKQVHPDTGISSKAMGIMNSFVNDIFERIAGEASRLAHYNKRSTITSREIQTAVRLLLP
                     GELAKHAVSEGTKAITKYTSAK"
     regulatory      468..477
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             474..1169
                     /codon_start=1
                     /product="Zoanthus green fluorescent protein"
                     /label=ZsGreen1
                     /note="mammalian codon-optimized"
                     /translation="MAQSKHGLTKEMTMKYRMEGCVDGHKFVITGEGIGYPFKGKQAIN
                     LCVVEGGPLPFAEDILSAAFMYGNRVFTEYPQDIVDYFKNSCPAGYTWDRSFLFEDGAV
                     CICNADITVSVEENCMYHESKFYGVNFPADGPVMKKMTDNWEPSCEKIIPVPKQGILKG
                     DVSMYLLLKDGGRLRCQFDTVYKAKSVPRKMPDWHFIQHKLTREDRSDAKNQKWHLTEH
                     AIASGSALP"
ORIGIN
        1 acgtcagatc cgctagcgct accggactca gatctcgagc tcaagcttcg aattctgcag
       61 tcgacggtac cgccaccatg ccagagccag cgaagtctgc tcccgccccg aaaaagggct
      121 ccaagaaggc ggtgactaag gcgcagaaga aaggcggcaa gaagcgcaag cgcagccgca
      181 aggagagcta ttccatctat gtgtacaagg ttctgaagca ggtccaccct gacaccggca
      241 tttcgtccaa ggccatgggc atcatgaatt cgtttgtgaa cgacattttc gagcgcatcg
      301 caggtgaggc ttcccgcctg gcgcattaca acaagcgctc gaccatcacc tccagggaga
      361 tccagacggc cgtgcgcctg ctgctgcctg gggagttggc caagcacgcc gtgtccgagg
      421 gtactaaggc catcaccaag tacaccagcg ctaaggatcc accggtcgcc accatggccc
      481 agtccaagca cggcctgacc aaggagatga ccatgaagta ccgcatggag ggctgcgtgg
      541 acggccacaa gttcgtgatc accggcgagg gcatcggcta ccccttcaag ggcaagcagg
      601 ccatcaacct gtgcgtggtg gagggcggcc ccttgccctt cgccgaggac atcttgtccg
      661 ccgccttcat gtacggcaac cgcgtgttca ccgagtaccc ccaggacatc gtcgactact
      721 tcaagaactc ctgccccgcc ggctacacct gggaccgctc cttcctgttc gaggacggcg
      781 ccgtgtgcat ctgcaacgcc gacatcaccg tgagcgtgga ggagaactgc atgtaccacg
      841 agtccaagtt ctacggcgtg aacttccccg ccgacggccc cgtgatgaag aagatgaccg
      901 acaactggga gccctcctgc gagaagatca tccccgtgcc caagcagggc atcttgaagg
      961 gcgacgtgag catgtacctg ctgctgaagg acggtggccg cttgcgctgc cagttcgaca
     1021 ccgtgtacaa ggccaagtcc gtgccccgca agatgcccga ctggcacttc atccagcaca
     1081 agctgacccg cgaggaccgc agcgacgcca agaaccagaa gtggcacctg accgagcacg
     1141 ccatcgcctc cggctccgcc ttgccctgag cggccgcgac tctagatcat aatcagccat
     1201 accacat
//
