LOCUS       sequence-87415-e        3528 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Alpha Actinin, Excitation: 505, Emission: 515.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 3528)
  AUTHORS   Lam AJ, St-Pierre F, Gong Y, Marshall JD, Cranfill PJ, Baird MA, 
            McKeown MR, Wiedenmann J, Davidson MW, Schnitzer MJ, Tsien RY, Lin 
            MZ
  TITLE     Improving FRET dynamic range with bright green and red fluorescent 
            proteins.
  JOURNAL   Nat Methods. 2012 Sep 9. doi: 10.1038/nmeth.2171.
  PUBMED    22961245
REFERENCE   2  (bases 1 to 3528)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Methods. 2012 Sep 9. doi: 10.1038/nmeth.2171."
COMMENT     Sequence Label: Clover-Alpha-Actinin-19
FEATURES             Location/Qualifiers
     source          1..3528
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      2768..2777
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             2774..3493
                     /codon_start=1
                     /product="monomeric derivative of the bright green-yellow 
                     fluorescent protein Clover"
                     /label=mClover2
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVRGEGEGDATNGKLTL
                     KFICTTGKLPVPWPTLVTTFGYGVACFSRYPDHMKQHDFFKSAMPEGYVQERTISFKDD
                     GTYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNFNSHNVYITADKQKNGIK
                     ANFKIRHNVEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSHQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(2819..2840)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     3427..3448
                     /label=EGFP-C
                     /note="EGFP, forward primer"
ORIGIN
        1 gtcagatccg ctagcgctac cggactcaga tctcgagctc aagcttcgca ccatcatgga
       61 ccattatgat tctcagcaaa ccaacgatta catgcagcca gaagaggact gggaccggga
      121 cctgctcctg gacccggcct gggagaagca gcagagaaag acattcacgg catggtgtaa
      181 ctcccacctc cggaaggcgg ggacacagat cgagaacatc gaagaggact tccgggatgg
      241 cctgaagctc atgctgctgc tggaggtcat ctcaggtgaa cgcttggcca agccagagcg
      301 aggcaagatg agagtgcaca agatctccaa cgtcaacaag gccctggatt tcatagccag
      361 caaaggcgtc aaactggtgt ccatcggagc cgaagaaatc gtggatggga atgtgaagat
      421 gaccctgggc atgatctgga ccatcatcct gcgctttgcc atccaggaca tctccgtgga
      481 agagacttca gccaaggaag ggctgctcct gtggtgtcag agaaagacag ccccttacaa
      541 aaatgtcaac atccagaact tccacataag ctggaaggat ggcctcggct tctgtgcttt
      601 gatccaccga caccggcccg agctgattga ctacgggaag ctgcggaagg atgatccact
      661 cacaaatctg aatacggctt ttgacgtggc agagaagtac ctggacatcc ccaagatgct
      721 ggatgccgaa gacatcgttg gaactgcccg accggatgag aaagccatca tgacttacgt
      781 gtctagcttc taccacgcct tctctggagc ccagaaggcg gagacagcag ccaatcgcat
      841 ctgcaaggtg ttggccgtca accaggagaa cgagcagctt atggaagact acgagaagct
      901 ggccagtgat ctgttggagt ggatccgccg cacaatcccg tggctggaga accgggtgcc
      961 cgagaacacc atgcatgcca tgcaacagaa gctggaggac ttccgggact accggcgcct
     1021 gcacaagccg cccaaggtgc aggagaagtg ccagctggag atcaacttca acacgctgca
     1081 gaccaagctg cggctcagca accggcctgc cttcatgccc tctgagggca ggatggtctc
     1141 ggacatcaac aatgcctggg gctgcctgga gcaggtggag aagggctatg aggagtggtt
     1201 gctgaatgag atccggaggc tggagcgact ggaccacctg gcagagaagt tccggcagaa
     1261 ggcctccatc cacgaggcct ggactgacgg caaagaggcc atgctgcgac agaaggacta
     1321 tgagaccgcc accctctcgg agatcaaggc cttgctcaag aagcatgagg ccttcgagag
     1381 tgacctggct gcccaccagg accgtgtgga gcagattgcc gccatcgcac aggagctcaa
     1441 tgagctggac tattatgact cacccagtgt caacgcccgt tgccaaaaga tctgtgacca
     1501 gtgggacaat ctgggggccc taactcagaa gcgaagggaa gctctggagc ggaccgagaa
     1561 actgctggag accattgacc agctgtactt ggagtatgcc aagcgggctg cacccttcaa
     1621 caactggatg gagggggcca tggaggacct gcaggacacc ttcattgtgc acaccattga
     1681 ggagatccag ggactgacca cagcccatga gcagttcaag gccaccctcc ctgatgccga
     1741 caaggagcgc ctggccatcc tgggcatcca caatgaggtg tccaagattg tccagaccta
     1801 ccacgtcaat atggcgggca ccaaccccta cacaaccatc acgcctcagg agatcaatgg
     1861 caaatgggac cacgtgcggc agctggtgcc tcggagggac caagctctga cggaggagca
     1921 tgcccgacag cagcacaatg agaggctacg caagcagttt ggagcccagg ccaatgtcat
     1981 cgggccctgg atccagacca agatggagga gatcgggagg atctccattg agatgcatgg
     2041 gaccctggag gaccagctca gccacctgcg gcagtatgag aagagcatcg tcaactacaa
     2101 gccaaagatt gatcagctgg agggcgacca ccagctcatc caggaggcgc tcatcttcga
     2161 caacaagcac accaactaca ccatggagca catccgtgtg ggctgggagc agctgctcac
     2221 caccatcgcc aggaccatca atgaggtaga gaaccagatc ctgacccggg atgccaaggg
     2281 catcagccag gagcagatga atgagttccg ggcctccttc aaccactttg accggaagaa
     2341 gacaggcatg atggacacgg atgatttccg cgcctgcctg atctccatgg gttacaacat
     2401 gggagaagca gaatttgccc gcatcatgag cattgtggac cccaaccgcc tgggggtagt
     2461 gacattccag gccttcattg acttcatgtc ccgcgagaca gccgacacag atacagcaga
     2521 ccaagtcatg gcttccttca agatcctggc tggggacaag aactacatta ccatggacga
     2581 gctgcgccgc gagctgccac ccgaccaggc tgagtactgc atcgcgcgga tggcccccta
     2641 caccggcccc gactccgtgc caggtgctct ggactacatg tccttctcca cggcgctgta
     2701 cggcgagagt gacctcaagc ttcgaattct gcagtcgacg gtaccgcggg cccgggatcc
     2761 accggtcgcc accatggtga gcaagggcga ggagctgttc accggggtgg tgcccatcct
     2821 ggtcgagctg gacggcgacg taaacggcca caagttcagc gtccgcggcg agggcgaggg
     2881 cgatgccacc aacggcaagc tgaccctgaa gttcatctgc accaccggca agctgcccgt
     2941 gccctggccc accctcgtga ccaccttcgg ctacggcgtg gcctgcttca gccgctaccc
     3001 cgaccacatg aagcagcacg acttcttcaa gtccgccatg cccgaaggct acgtccagga
     3061 gcgcaccatc tctttcaagg acgacggtac ctacaagacc cgcgccgagg tgaagttcga
     3121 gggcgacacc ctggtgaacc gcatcgagct gaagggcatc gacttcaagg aggacggcaa
     3181 catcctgggg cacaagctgg agtacaactt caacagccac aacgtctata tcacggccga
     3241 caagcagaag aacggcatca aggctaactt caagatccgc cacaacgttg aggacggcag
     3301 cgtgcagctc gccgaccact accagcagaa cacccccatc ggcgacggcc ccgtgctgct
     3361 gcccgacaac cactacctga gccatcagtc cgccctgagc aaagacccca acgagaagcg
     3421 cgatcacatg gtcctgctgg agttcgtgac cgccgccggg atcactctcg gcatggacga
     3481 gctgtacaag taagcggccg cgactctaga tcataatcag ccatacca
//
