LOCUS       sequence-87416-e        2156 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Gap Junctions, Excitation: 505, Emission: 515.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2156)
  AUTHORS   Lam AJ, St-Pierre F, Gong Y, Marshall JD, Cranfill PJ, Baird MA, 
            McKeown MR, Wiedenmann J, Davidson MW, Schnitzer MJ, Tsien RY, Lin 
            MZ
  TITLE     Improving FRET dynamic range with bright green and red fluorescent 
            proteins.
  JOURNAL   Nat Methods. 2012 Sep 9. doi: 10.1038/nmeth.2171.
  PUBMED    22961245
REFERENCE   2  (bases 1 to 2156)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Methods. 2012 Sep 9. doi: 10.1038/nmeth.2171."
COMMENT     Sequence Label: Clover-Cx43-7
FEATURES             Location/Qualifiers
     source          1..2156
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      1397..1406
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             1403..2122
                     /codon_start=1
                     /product="monomeric derivative of the bright green-yellow 
                     fluorescent protein Clover"
                     /label=mClover2
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVRGEGEGDATNGKLTL
                     KFICTTGKLPVPWPTLVTTFGYGVACFSRYPDHMKQHDFFKSAMPEGYVQERTISFKDD
                     GTYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNFNSHNVYITADKQKNGIK
                     ANFKIRHNVEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSHQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(1448..1469)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     2056..2077
                     /label=EGFP-C
                     /note="EGFP, forward primer"
ORIGIN
        1 ccgctagcgc taccggactc agatctcgag ctcaagcttc gaattcggca gcacttttct
       61 ttcattgggg ggaaggcgtg aggaaagtac caaacagcag cagactttta aactttaaac
      121 agacaggtct gagagcctga actctcattt ttcctttgac ttcagcctcc aaggagttcc
      181 accaactttg gcgcgccggc ttcactttca ttaagtgaaa gagaggtgcc cagacatggg
      241 tgactggagt gccttgggga agcttctgga caaggtccaa gcctactcca ccgctggagg
      301 gaaggtgtgg ctgtcagtgc tcttcatatt cagaatcctg ctcctgggga cagctgttga
      361 gtcagcttgg ggtgatgaac agtctgcctt tcgctgtaac actcaacaac ctggctgcga
      421 aaacgtctgc tatgacaagt ccttccccat ctctcacgtg cgcttctggg tccttcagat
      481 catattcgtg tctgtgccca cactcctgta cttggcccat gtgttctatg tgatgaggaa
      541 ggaagagaag ctaaacaaga aagaagagga gctcaaagtg gcccagactg acggggtcaa
      601 cgtggagatg cacctgaagc agattgaaat caagaagttc aagtacggga ttgaagagca
      661 cggcaaggtg aaaatgaggg gcggcttgct gagaacctac atcatcagca tcctcttcaa
      721 gtctgtcttc gaggtggcct tcctgctcat ccagtggtac atctatgggt tcagcttgag
      781 cgcggtctac acctgcaaga gagatccctg cccccaccag gtagactgct tcctctcacg
      841 tcccacggag aaaaccatct tcatcatctt catgctggtg gtgtccttgg tgtctctcgc
      901 tttgaacatc attgagctct tctacgtctt cttcaaaggc gttaaggatc gcgtgaaggg
      961 aagaagcgat ccttaccacg ccaccactgg cccactgagc ccatcaaaag actgcggatc
     1021 tccaaaatac gcctacttca atggctgctc ctcaccaacg gctccactct cgcctatgtc
     1081 tcctcctggg tacaagctgg ttactggtga cagaaacaat tcctcgtgcc gcaattacaa
     1141 caagcaagct agcgagcaaa actgggcgaa ctacagcgca gagcaaaatc gcatggggca
     1201 ggccggaagc accatctcca actcgcacgc ccagccgttc gatttccccg acgacaacca
     1261 gaatgccaaa aaagttgctg ctggacatga actccagcca ttagccatcg tggaccaacg
     1321 accttccagc agagccagca gccgcgccag cagcaggcct cggcctgatg acctggagat
     1381 tgcggatcca ccggtcgcca ccatggtgag caagggcgag gagctgttca ccggggtggt
     1441 gcccatcctg gtcgagctgg acggcgacgt aaacggccac aagttcagcg tccgcggcga
     1501 gggcgagggc gatgccacca acggcaagct gaccctgaag ttcatctgca ccaccggcaa
     1561 gctgcccgtg ccctggccca ccctcgtgac caccttcggc tacggcgtgg cctgcttcag
     1621 ccgctacccc gaccacatga agcagcacga cttcttcaag tccgccatgc ccgaaggcta
     1681 cgtccaggag cgcaccatct ctttcaagga cgacggtacc tacaagaccc gcgccgaggt
     1741 gaagttcgag ggcgacaccc tggtgaaccg catcgagctg aagggcatcg acttcaagga
     1801 ggacggcaac atcctggggc acaagctgga gtacaacttc aacagccaca acgtctatat
     1861 cacggccgac aagcagaaga acggcatcaa ggctaacttc aagatccgcc acaacgttga
     1921 ggacggcagc gtgcagctcg ccgaccacta ccagcagaac acccccatcg gcgacggccc
     1981 cgtgctgctg cccgacaacc actacctgag ccatcagtcc gccctgagca aagaccccaa
     2041 cgagaagcgc gatcacatgg tcctgctgga gttcgtgacc gccgccggga tcactctcgg
     2101 catggacgag ctgtacaagt aagcggccgc gactctagat cataatcagc catacc
//
