LOCUS       sequence-87423-e        1457 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Ras GTPase, Excitation: 505, Emission: 515.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1457)
  AUTHORS   Lam AJ, St-Pierre F, Gong Y, Marshall JD, Cranfill PJ, Baird MA, 
            McKeown MR, Wiedenmann J, Davidson MW, Schnitzer MJ, Tsien RY, Lin 
            MZ
  TITLE     Improving FRET dynamic range with bright green and red fluorescent 
            proteins.
  JOURNAL   Nat Methods. 2012 Sep 9. doi: 10.1038/nmeth.2171.
  PUBMED    22961245
REFERENCE   2  (bases 1 to 1457)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Methods. 2012 Sep 9. doi: 10.1038/nmeth.2171."
COMMENT     Sequence Label: Clover-Rab5a-7
FEATURES             Location/Qualifiers
     source          1..1457
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      17..26
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             23..739
                     /codon_start=1
                     /product="monomeric derivative of the bright green-yellow 
                     fluorescent protein Clover"
                     /label=mClover2
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVRGEGEGDATNGKLTL
                     KFICTTGKLPVPWPTLVTTFGYGVACFSRYPDHMKQHDFFKSAMPEGYVQERTISFKDD
                     GTYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNFNSHNVYITADKQKNGIK
                     ANFKIRHNVEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSHQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(68..89)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     676..697
                     /label=EGFP-C
                     /note="EGFP, forward primer"
ORIGIN
        1 gctagcgcta ccggtcgcca ccatggtgag caagggcgag gagctgttca ccggggtggt
       61 gcccatcctg gtcgagctgg acggcgacgt aaacggccac aagttcagcg tccgcggcga
      121 gggcgagggc gatgccacca acggcaagct gaccctgaag ttcatctgca ccaccggcaa
      181 gctgcccgtg ccctggccca ccctcgtgac caccttcggc tacggcgtgg cctgcttcag
      241 ccgctacccc gaccacatga agcagcacga cttcttcaag tccgccatgc ccgaaggcta
      301 cgtccaggag cgcaccatct ctttcaagga cgacggtacc tacaagaccc gcgccgaggt
      361 gaagttcgag ggcgacaccc tggtgaaccg catcgagctg aagggcatcg acttcaagga
      421 ggacggcaac atcctggggc acaagctgga gtacaacttc aacagccaca acgtctatat
      481 cacggccgac aagcagaaga acggcatcaa ggctaacttc aagatccgcc acaacgttga
      541 ggacggcagc gtgcagctcg ccgaccacta ccagcagaac acccccatcg gcgacggccc
      601 cgtgctgctg cccgacaacc actacctgag ccatcagtcc gccctgagca aagaccccaa
      661 cgagaagcgc gatcacatgg tcctgctgga gttcgtgacc gccgccggga tcactctcgg
      721 catggacgag ctgtacaagt ccggactcag atctcgagcc atggctagtc gaggcgcaac
      781 aagacccaac gggccaaata ctggaaataa aatatgccag ttcaaactag tacttctggg
      841 agagtctgct gttggcaaat caagcctagt gcttcgtttt gtgaagggcc aatttcatga
      901 atttcaagag agtaccatag gggctgcttt tctaacccaa actgtgtgtc ttgatgatac
      961 aacagtaaag tttgaaatat gggatacagc tggtcaagaa cgataccata gcttagcacc
     1021 aatgtactac agaggagcac aagcagccat agttgtatat gatatcacaa atgaggagtc
     1081 ctttgccaga gccaaaaact gggttaaaga acttcagagg caagccagtc ctaacattgt
     1141 aatagcttta tcaggaaaca aggctgatct tgcaaataaa agagctgtcg atttccagga
     1201 agcacagtcc tatgcagatg acaacagttt attattcatg gagacatcag ctaaaacatc
     1261 gatgaatgta aatgaaatat tcatggcaat agctaaaaag ttgccaaaga acgaaccaca
     1321 gaatccagga gcaaattctg ccagaggaag aggagtagac cttactgaac ccacgcagcc
     1381 aaccaggaat cagtgttgta gtaactaagg atccaccgga tctagataac tgatcataat
     1441 cagccatacc acattgt
//
