LOCUS       sequence-87424-e        2547 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Focal Adhesions, Excitation: 505, Emission: 515.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2547)
  AUTHORS   Lam AJ, St-Pierre F, Gong Y, Marshall JD, Cranfill PJ, Baird MA, 
            McKeown MR, Wiedenmann J, Davidson MW, Schnitzer MJ, Tsien RY, Lin 
            MZ
  TITLE     Improving FRET dynamic range with bright green and red fluorescent 
            proteins.
  JOURNAL   Nat Methods. 2012 Sep 9. doi: 10.1038/nmeth.2171.
  PUBMED    22961245
REFERENCE   2  (bases 1 to 2547)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Methods. 2012 Sep 9. doi: 10.1038/nmeth.2171."
COMMENT     Sequence Label: Clover-Zyxin-6
FEATURES             Location/Qualifiers
     source          1..2547
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      62..71
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     regulatory      1792..1801
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             1798..2517
                     /codon_start=1
                     /product="monomeric derivative of the bright green-yellow 
                     fluorescent protein Clover"
                     /label=mClover2
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVRGEGEGDATNGKLTL
                     KFICTTGKLPVPWPTLVTTFGYGVACFSRYPDHMKQHDFFKSAMPEGYVQERTISFKDD
                     GTYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNFNSHNVYITADKQKNGIK
                     ANFKIRHNVEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSHQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(1843..1864)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     2451..2472
                     /label=EGFP-C
                     /note="EGFP, forward primer"
ORIGIN
        1 cctagtgacg tcagatccgc tagcgctacc ggactcagat ctcgagctca agcttcgaat
       61 tccatggcgg ccccccgccc gtctcccgcg atccccgttt cggtctcggc tccggctttt
      121 tacgccccgc agaagaagtt cggccctgtg gtggccccaa agcccaaagt gaatcccttc
      181 cggcccaggg acagcgagcc tcccccggca cccggggccc agcgcgcaca gatgggccgg
      241 gtgggcgata ttcccccgcc gcccccggaa gactttcccc tgcctccacc tccccttgct
      301 ggggatggcg acgatgcaga gggtgctctg ggaggtgcct tcccgccgcc ccctcccccg
      361 atcgaggaat catttccccc tgcgcctctg gaggaggaga tcttcccttc cccgccgcct
      421 cctccggagg aggagggagg gcctgaggcc cccataccgc ccccaccaca gcccagggag
      481 aaggtgagca gtattgattt ggagatcgac tctctgtcct cactgctgga tgacatgacc
      541 aagaatgatc ctttcaaagc ccgggtgtca tctggatatg tgcccccacc agtggccact
      601 ccattcagtt ccaagtccag taccaagcct gcagccgggg gcacagcacc cctgcctcct
      661 tggaagtccc cttccagctc ccagcctctg ccccaggttc cggctccggc tcagagccag
      721 acacagttcc atgttcagcc ccagccccag cccaagcctc aggtccaact ccatgtccag
      781 tcccagaccc agcctgtgtc tttggctaac acccagcccc gagggccccc agcctcatct
      841 ccggctccag cccctaagtt ttctccagtg actcctaagt ttactcctgt ggcttccaag
      901 ttcagtcctg gagccccagg tggatctggg tcacaaccaa atcaaaaatt ggggcacccc
      961 gaagctcttt ctgctggcac aggctcccct caacctccca gcttcaccta tgcccagcag
     1021 agggagaagc cccgagtgca ggagaagcag caccccgtgc ccccaccggc tcagaaccaa
     1081 aaccaggtgc gctcccctgg ggccccaggg cccctgactc tgaaggaggt ggaggagctg
     1141 gagcagctga cccagcagct aatgcaggac atggagcatc ctcagaggca gaatgtggct
     1201 gtcaacgaac tctgcggccg atgccatcaa cccctggccc gggcgcagcc agccgtccgc
     1261 gctctagggc agctgttcca catcgcctgc ttcacctgcc accagtgtgc gcagcagctc
     1321 cagggccagc agttctacag tctggagggg gcgccgtact gcgagggctg ttacactgac
     1381 accctggaga agtgtaacac ctgcggggag cccatcactg accgcatgct gagggccacg
     1441 ggcaaggcct atcacccgca ctgcttcacc tgtgtggtct gcgcccgccc cctggagggc
     1501 acctccttca tcgtggacca ggccaaccgg ccccactgtg tccccgacta ccacaagcag
     1561 tacgccccga ggtgctccgt ctgctctgag cccatcatgc ctgagcctgg ccgagatgag
     1621 actgtgcgag tggtcgccct ggacaagaac ttccacatga agtgttacaa gtgtgaggac
     1681 tgcgggaagc ccctgtcgat tgaggcagat gacaatggct gcttccccct ggacggtcac
     1741 gtgctctgtc ggaagtgcca cactgctaga gcccagacgg atccaccggt cgccaccatg
     1801 gtgagcaagg gcgaggagct gttcaccggg gtggtgccca tcctggtcga gctggacggc
     1861 gacgtaaacg gccacaagtt cagcgtccgc ggcgagggcg agggcgatgc caccaacggc
     1921 aagctgaccc tgaagttcat ctgcaccacc ggcaagctgc ccgtgccctg gcccaccctc
     1981 gtgaccacct tcggctacgg cgtggcctgc ttcagccgct accccgacca catgaagcag
     2041 cacgacttct tcaagtccgc catgcccgaa ggctacgtcc aggagcgcac catctctttc
     2101 aaggacgacg gtacctacaa gacccgcgcc gaggtgaagt tcgagggcga caccctggtg
     2161 aaccgcatcg agctgaaggg catcgacttc aaggaggacg gcaacatcct ggggcacaag
     2221 ctggagtaca acttcaacag ccacaacgtc tatatcacgg ccgacaagca gaagaacggc
     2281 atcaaggcta acttcaagat ccgccacaac gttgaggacg gcagcgtgca gctcgccgac
     2341 cactaccagc agaacacccc catcggcgac ggccccgtgc tgctgcccga caaccactac
     2401 ctgagccatc agtccgccct gagcaaagac cccaacgaga agcgcgatca catggtcctg
     2461 ctggagttcg tgaccgccgc cgggatcact ctcggcatgg acgagctgta caagtaagcg
     2521 gccgcgactc tagatcataa tcagcca
//
