LOCUS       sequence-87429-e        2169 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Microtubules, Excitation: 505, Emission: 515.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2169)
  TITLE     Davidson Green fluorescent proteins
REFERENCE   2  (bases 1 to 2169)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: Clover2-Tubulin-6
FEATURES             Location/Qualifiers
     source          1..2169
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      27..36
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             33..749
                     /codon_start=1
                     /product="monomeric derivative of the bright green-yellow 
                     fluorescent protein Clover"
                     /label=mClover2
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVRGEGEGDATNGKLTL
                     KFICTTGKLPVPWPTLVTTFGYGVACFSRYPDHMKQHDFFKSAMPEGYVQERTISFKDD
                     GTYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNFNSHYVYITADKQKNSIK
                     ANFKIRHNVEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSHQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(78..99)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     686..707
                     /label=EGFP-C
                     /note="EGFP, forward primer"
ORIGIN
        1 cgtcagatcc gctagcgcta ccggtcgcca ccatggtgag caagggcgag gagctgttca
       61 ccggggtggt gcccatcctg gtcgagctgg acggcgacgt aaacggccac aagttcagcg
      121 tccgcggcga gggcgagggc gatgccacca acggcaagct gaccctgaag ttcatctgca
      181 ccaccggcaa gctgcccgtg ccctggccca ccctcgtgac caccttcggc tacggcgtgg
      241 cctgcttcag ccgctacccc gaccacatga agcagcacga cttcttcaag tccgccatgc
      301 ccgaaggcta cgtccaggag cgcaccatct ctttcaagga cgacggtacc tacaagaccc
      361 gcgccgaggt gaagttcgag ggcgacaccc tggtgaaccg catcgagctg aagggcatcg
      421 acttcaagga ggacggcaac atcctggggc acaagctgga gtacaacttc aacagccact
      481 acgtctatat cacggccgac aagcagaaga acagcatcaa ggctaacttc aagatccgcc
      541 acaacgttga ggacggcagc gtgcagctcg ccgaccacta ccagcagaac acccccatcg
      601 gcgacggccc cgtgctgctg cccgacaacc actacctgag ccatcagtcc gccctgagca
      661 aagaccccaa cgagaagcgc gatcacatgg tcctgctgga gttcgtgacc gccgccggga
      721 tcactctcgg catggacgag ctgtacaagt ccggactcag atctcgagtg cgtgagtgca
      781 tctccatcca cgttggccag gctggtgtcc agattggcaa tgcctgctgg gagctctact
      841 gcctggaaca cggcatccag cccgatggcc agatgccaag tgacaagacc attgggggag
      901 gagatgactc cttcaacacc ttcttcagtg agacgggcgc tggcaagcac gtgccccggg
      961 ctgtgtttgt agacttggaa cccacagtca ttgatgaagt tcgcactggc acctaccgcc
     1021 agctcttcca ccctgagcag ctcatcacag gcaaggaaga tgctgccaat aactatgccc
     1081 gagggcacta caccattggc aaggagatca ttgaccttgt gttggaccga attcgcaagc
     1141 tggctgacca gtgcaccggt cttcagggct tcttggtttt ccacagcttt ggtgggggaa
     1201 ctggttctgg gttcacctcc ctgctcatgg aacgtctctc agttgattat ggcaagaagt
     1261 ccaagctgga gttctccatt tacccagcac cccaggtttc cacagctgta gttgagccct
     1321 acaactccat cctcaccacc cacaccaccc tggagcactc tgattgtgcc ttcatggtag
     1381 acaatgaggc catctatgac atctgtcgta gaaacctcga tatcgagcgc ccaacctaca
     1441 ctaaccttaa ccgccttatt agccagattg tgtcctccat cactgcttcc ctgagatttg
     1501 atggagccct gaatgttgac ctgacagaat tccagaccaa cctggtgccc tacccccgca
     1561 tccacttccc tctggccaca tatgcccctg tcatctctgc tgagaaagcc taccatgaac
     1621 agctttctgt agcagagatc accaatgctt gctttgagcc agccaaccag atggtgaaat
     1681 gtgaccctcg ccatggtaaa tacatggctt gctgcctgtt gtaccgtggt gacgtggttc
     1741 ccaaagatgt caatgctgcc attgccacca tcaaaaccaa gcgcagcatc cagtttgtgg
     1801 attggtgccc cactggcttc aaggttggca tcaactacca gcctcccact gtggtgcctg
     1861 gtggagacct ggccaaggta cagagagctg tgtgcatgct gagcaacacc acagccattg
     1921 ctgaggcctg ggctcgcctg gaccacaagt ttgacctgat gtatgccaag cgtgcctttg
     1981 ttcactggta cgtgggtgag gggatggagg aaggcgagtt ttcagaggcc cgtgaagata
     2041 tggctgccct tgagaaggat tatgaggagg ttggtgtgga ttctgttgaa ggagagggtg
     2101 aggaagaagg agaggaatac taaggatcca ccggatctag ataactgatc ataatcagcc
     2161 ataccacat
//
