LOCUS       sequence-87430-e        1948 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Actin, Excitation: 406, Emission: 526.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1948)
  TITLE     Davidson Yellow fluorescent proteins
REFERENCE   2  (bases 1 to 1948)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: mAmetrine-Actin-7
FEATURES             Location/Qualifiers
     source          1..1948
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      30..39
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(36..38,39..41,42..752)
                     /codon_start=1
                     /product="yellow fluorescent protein with a large Stokes 
                     shift, excitable with violet light (Ai et al., 2008)"
                     /label=mAmetrine
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVRGEGEGDATNGKLTL
                     KFICTSGKLPVPWPTLVTTLSYGVQCFARYPDHMKQHDFFKSAMPEGYVQERTISFKDD
                     GSYRTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNMNVWDAYITADKQKNGIK
                     ANFKIEHNVEDGGVQLADAYQQNTPIGDGSVLLPDNHYLSFQSKLFKDPNEQRDHMVLL
                     EFVTAAGITPGMDELYK"
     primer_bind     complement(81..102)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
ORIGIN
        1 tgacgtcaga tccgctagcg ctaccggtcg ccaccatggt gagcaagggc gaggagctgt
       61 tcaccggggt ggtgcccatc ctggtcgagc tggacggcga cgtaaacggc cacaagttca
      121 gcgtgcgcgg cgagggcgag ggcgatgcca ccaacggcaa gctgaccctg aagttcatct
      181 gcacctccgg caagctgccc gtgccctggc ccaccctcgt gaccaccctg tcttacggcg
      241 tgcagtgctt cgcccgctac cccgaccaca tgaagcagca cgacttcttc aagtccgcca
      301 tgcccgaagg ctacgtccag gagcgcacca tctccttcaa ggacgacggc agctacagga
      361 cccgcgccga ggtgaagttc gagggcgaca ccctggtgaa ccgcatcgag ctgaagggca
      421 tcgacttcaa ggaggacggc aacatcctgg ggcacaagct ggagtacaac atgaacgtgt
      481 gggacgcgta tatcacggcc gacaagcaga agaacggcat caaagcgaac ttcaagatcg
      541 agcacaacgt cgaggacggc ggcgtgcagc tcgccgacgc gtaccagcag aacaccccca
      601 tcggcgacgg ctccgtgctg ctgcctgaca accactacct gagcttccag agcaagctgt
      661 tcaaagaccc caacgagcag cgcgatcaca tggtcctgct ggagttcgtt accgccgccg
      721 ggatcactcc cggcatggac gagctgtaca agtccggact cagatctcga gctatggatg
      781 atgatatcgc cgcgctcgtc gtcgacaacg gctccggcat gtgcaaggcc ggcttcgcgg
      841 gcgacgatgc cccccgggcc gtcttcccct ccatcgtggg gcgccccagg caccagggcg
      901 tgatggtggg catgggtcag aaggattcct atgtgggcga cgaggcccag agcaagagag
      961 gcatcctcac cctgaagtac cccatcgagc acggcatcgt caccaactgg gacgacatgg
     1021 agaaaatctg gcaccacacc ttctacaatg agctgcgtgt ggctcccgag gagcaccccg
     1081 tgctgctgac cgaggccccc ctgaacccca aggccaaccg cgagaagatg acccagatca
     1141 tgtttgagac cttcaacacc ccagccatgt acgttgctat ccaggctgtg ctatccctgt
     1201 acgcctctgg ccgtaccact ggcatcgtga tggactccgg tgacggggtc acccacactg
     1261 tgcccatcta cgaggggtat gccctccccc atgccatcct gcgtctggac ctggctggcc
     1321 gggacctgac tgactacctc atgaagatcc tcaccgagcg cggctacagc ttcaccacca
     1381 cggccgagcg ggaaatcgtg cgtgacatta aggagaagct gtgctacgtc gccctggact
     1441 tcgagcaaga gatggccacg gctgcttcca gctcctccct ggagaagagc tacgagctgc
     1501 ctgacggcca ggtcatcacc attggcaatg agcggttccg ctgccctgag gcactcttcc
     1561 agccttcctt cctgggcatg gagtcctgtg gcatccacga aactaccttc aactccatca
     1621 tgaagtgtga cgtggacatc cgcaaagacc tgtacgccaa cacagtgctg tctggcggca
     1681 ccaccatgta ccctggcatt gccgacagga tgcagaagga gatcactgcc ctggcaccca
     1741 gcacaatgaa gatcaagatc attgctcctc ctgagcgcaa gtactccgtg tggatcggcg
     1801 gctccatcct ggcctcgctg tccaccttcc agcagatgtg gatcagcaag caggagtatg
     1861 acgagtccgg cccctccatc gtccaccgca aatgcttcta gggatccacc ggatctagat
     1921 aactgatcat aatcagccat accacatt
//
