LOCUS       sequence-87433-e        2169 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Gap Junctions, Excitation: 406, Emission: 526.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2169)
  TITLE     Davidson Yellow fluorescent proteins
REFERENCE   2  (bases 1 to 2169)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: mAmetrine-Cx43-7
FEATURES             Location/Qualifiers
     source          1..2169
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      1400..1409
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(1406..1408,1409..1411,1412..2125)
                     /codon_start=1
                     /product="yellow fluorescent protein with a large Stokes 
                     shift, excitable with violet light (Ai et al., 2008)"
                     /label=mAmetrine
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVRGEGEGDATNGKLTL
                     KFICTSGKLPVPWPTLVTTLSYGVQCFARYPDHMKQHDFFKSAMPEGYVQERTISFKDD
                     GSYRTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNMNVWDAYITADKQKNGIK
                     ANFKIEHNVEDGGVQLADAYQQNTPIGDGSVLLPDNHYLSFQSKLFKDPNEQRDHMVLL
                     EFVTAAGITPGMDELYK"
     primer_bind     complement(1451..1472)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
ORIGIN
        1 gatccgctag cgctaccgga ctcagatctc gagctcaagc ttcgaattcg gcagcacttt
       61 tctttcattg gggggaaggc gtgaggaaag taccaaacag cagcagactt ttaaacttta
      121 aacagacagg tctgagagcc tgaactctca tttttccttt gacttcagcc tccaaggagt
      181 tccaccaact ttggcgcgcc ggcttcactt tcattaagtg aaagagaggt gcccagacat
      241 gggtgactgg agtgccttgg ggaagcttct ggacaaggtc caagcctact ccaccgctgg
      301 agggaaggtg tggctgtcag tgctcttcat attcagaatc ctgctcctgg ggacagctgt
      361 tgagtcagct tggggtgatg aacagtctgc ctttcgctgt aacactcaac aacctggctg
      421 cgaaaacgtc tgctatgaca agtccttccc catctctcac gtgcgcttct gggtccttca
      481 gatcatattc gtgtctgtgc ccacactcct gtacttggcc catgtgttct atgtgatgag
      541 gaaggaagag aagctaaaca agaaagaaga ggagctcaaa gtggcccaga ctgacggggt
      601 caacgtggag atgcacctga agcagattga aatcaagaag ttcaagtacg ggattgaaga
      661 gcacggcaag gtgaaaatga ggggcggctt gctgagaacc tacatcatca gcatcctctt
      721 caagtctgtc ttcgaggtgg ccttcctgct catccagtgg tacatctatg ggttcagctt
      781 gagcgcggtc tacacctgca agagagatcc ctgcccccac caggtagact gcttcctctc
      841 acgtcccacg gagaaaacca tcttcatcat cttcatgctg gtggtgtcct tggtgtctct
      901 cgctttgaac atcattgagc tcttctacgt cttcttcaaa ggcgttaagg atcgcgtgaa
      961 gggaagaagc gatccttacc acgccaccac tggcccactg agcccatcaa aagactgcgg
     1021 atctccaaaa tacgcctact tcaatggctg ctcctcacca acggctccac tctcgcctat
     1081 gtctcctcct gggtacaagc tggttactgg tgacagaaac aattcctcgt gccgcaatta
     1141 caacaagcaa gctagcgagc aaaactgggc gaactacagc gcagagcaaa atcgcatggg
     1201 gcaggccgga agcaccatct ccaactcgca cgcccagccg ttcgatttcc ccgacgacaa
     1261 ccagaatgcc aaaaaagttg ctgctggaca tgaactccag ccattagcca tcgtggacca
     1321 acgaccttcc agcagagcca gcagccgcgc cagcagcagg cctcggcctg atgacctgga
     1381 gattgcggat ccaccggtcg ccaccatggt gagcaagggc gaggagctgt tcaccggggt
     1441 ggtgcccatc ctggtcgagc tggacggcga cgtaaacggc cacaagttca gcgtgcgcgg
     1501 cgagggcgag ggcgatgcca ccaacggcaa gctgaccctg aagttcatct gcacctccgg
     1561 caagctgccc gtgccctggc ccaccctcgt gaccaccctg tcttacggcg tgcagtgctt
     1621 cgcccgctac cccgaccaca tgaagcagca cgacttcttc aagtccgcca tgcccgaagg
     1681 ctacgtccag gagcgcacca tctccttcaa ggacgacggc agctacagga cccgcgccga
     1741 ggtgaagttc gagggcgaca ccctggtgaa ccgcatcgag ctgaagggca tcgacttcaa
     1801 ggaggacggc aacatcctgg ggcacaagct ggagtacaac atgaacgtgt gggacgcgta
     1861 tatcacggcc gacaagcaga agaacggcat caaagcgaac ttcaagatcg agcacaacgt
     1921 cgaggacggc ggcgtgcagc tcgccgacgc gtaccagcag aacaccccca tcggcgacgg
     1981 ctccgtgctg ctgcctgaca accactacct gagcttccag agcaagctgt tcaaagaccc
     2041 caacgagcag cgcgatcaca tggtcctgct ggagttcgtt accgccgccg ggatcactcc
     2101 cggcatggac gagctgtaca agtaagcggc cgcgactcta gatcataatc agccatacca
     2161 cattgagaa
//
