LOCUS       sequence-87434-e        1065 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Golgi, Excitation: 406, Emission: 526.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1065)
  TITLE     Davidson Yellow fluorescent proteins
REFERENCE   2  (bases 1 to 1065)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: mAmetrine-Golgi-7
FEATURES             Location/Qualifiers
     source          1..1065
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      314..323
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(320..322,323..325,326..1039)
                     /codon_start=1
                     /product="yellow fluorescent protein with a large Stokes 
                     shift, excitable with violet light (Ai et al., 2008)"
                     /label=mAmetrine
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVRGEGEGDATNGKLTL
                     KFICTSGKLPVPWPTLVTTLSYGVQCFARYPDHMKQHDFFKSAMPEGYVQERTISFKDD
                     GSYRTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNMNVWDAYITADKQKNGIK
                     ANFKIEHNVEDGGVQLADAYQQNTPIGDGSVLLPDNHYLSFQSKLFKDPNEQRDHMVLL
                     EFVTAAGITPGMDELYK"
     primer_bind     complement(365..386)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
ORIGIN
        1 ggggaggtct atataagcag agctggttta gtgaaccgtc agatccgcta gcatgaggct
       61 tcgggagccg ctcctgagcg gcagcgccgc gatgccaggc gcgtccctac agcgggcctg
      121 ccgcctgctc gtggccgtct gcgctctgca ccttggcgtc accctcgttt actacctggc
      181 tggccgcgac ctgagccgcc tgccccaact ggtcggagtc tccacaccgc tgcagggcgg
      241 ctcgaacagt gccgccgcca tcgggcagtc ctccggggag ctccggaccg gaggggccaa
      301 ggatccaccg gtcgccacca tggtgagcaa gggcgaggag ctgttcaccg gggtggtgcc
      361 catcctggtc gagctggacg gcgacgtaaa cggccacaag ttcagcgtgc gcggcgaggg
      421 cgagggcgat gccaccaacg gcaagctgac cctgaagttc atctgcacct ccggcaagct
      481 gcccgtgccc tggcccaccc tcgtgaccac cctgtcttac ggcgtgcagt gcttcgcccg
      541 ctaccccgac cacatgaagc agcacgactt cttcaagtcc gccatgcccg aaggctacgt
      601 ccaggagcgc accatctcct tcaaggacga cggcagctac aggacccgcg ccgaggtgaa
      661 gttcgagggc gacaccctgg tgaaccgcat cgagctgaag ggcatcgact tcaaggagga
      721 cggcaacatc ctggggcaca agctggagta caacatgaac gtgtgggacg cgtatatcac
      781 ggccgacaag cagaagaacg gcatcaaagc gaacttcaag atcgagcaca acgtcgagga
      841 cggcggcgtg cagctcgccg acgcgtacca gcagaacacc cccatcggcg acggctccgt
      901 gctgctgcct gacaaccact acctgagctt ccagagcaag ctgttcaaag accccaacga
      961 gcagcgcgat cacatggtcc tgctggagtt cgttaccgcc gccgggatca ctcccggcat
     1021 ggacgagctg tacaagtaag cggccgcgac tctagatcat aatca
//
