LOCUS       sequence-87435-e        1225 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Nucleus/Histones, Excitation: 406, Emission: 526.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1225)
  TITLE     Davidson Yellow fluorescent proteins
REFERENCE   2  (bases 1 to 1225)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: mAmetrine-H2B-6
FEATURES             Location/Qualifiers
     source          1..1225
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             74..451
                     /codon_start=1
                     /gene="HIST1H2BJ"
                     /product="human histone H2B"
                     /label=H2B
                     /translation="MPEPAKSAPAPKKGSKKAVTKAQKKGGKKRKRSRKESYSIYVYKV
                     LKQVHPDTGISSKAMGIMNSFVNDIFERIAGEASRLAHYNKRSTITSREIQTAVRLLLP
                     GELAKHAVSEGTKAITKYTSAK"
     regulatory      464..473
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(470..472,473..475,476..1189)
                     /codon_start=1
                     /product="yellow fluorescent protein with a large Stokes 
                     shift, excitable with violet light (Ai et al., 2008)"
                     /label=mAmetrine
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVRGEGEGDATNGKLTL
                     KFICTSGKLPVPWPTLVTTLSYGVQCFARYPDHMKQHDFFKSAMPEGYVQERTISFKDD
                     GSYRTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNMNVWDAYITADKQKNGIK
                     ANFKIEHNVEDGGVQLADAYQQNTPIGDGSVLLPDNHYLSFQSKLFKDPNEQRDHMVLL
                     EFVTAAGITPGMDELYK"
     primer_bind     complement(515..536)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
ORIGIN
        1 cagatccgct agcgctaccg gactcagatc tcgagctcaa gcttcgaatt ctgcagtcga
       61 cggtaccgcc accatgccag agccagcgaa gtctgctccc gccccgaaaa agggctccaa
      121 gaaggcggtg actaaggcgc agaagaaagg cggcaagaag cgcaagcgca gccgcaagga
      181 gagctattcc atctatgtgt acaaggttct gaagcaggtc caccctgaca ccggcatttc
      241 gtccaaggcc atgggcatca tgaattcgtt tgtgaacgac attttcgagc gcatcgcagg
      301 tgaggcttcc cgcctggcgc attacaacaa gcgctcgacc atcacctcca gggagatcca
      361 gacggccgtg cgcctgctgc tgcctgggga gttggccaag cacgccgtgt ccgagggtac
      421 taaggccatc accaagtaca ccagcgctaa ggatccaccg gtcgccacca tggtgagcaa
      481 gggcgaggag ctgttcaccg gggtggtgcc catcctggtc gagctggacg gcgacgtaaa
      541 cggccacaag ttcagcgtgc gcggcgaggg cgagggcgat gccaccaacg gcaagctgac
      601 cctgaagttc atctgcacct ccggcaagct gcccgtgccc tggcccaccc tcgtgaccac
      661 cctgtcttac ggcgtgcagt gcttcgcccg ctaccccgac cacatgaagc agcacgactt
      721 cttcaagtcc gccatgcccg aaggctacgt ccaggagcgc accatctcct tcaaggacga
      781 cggcagctac aggacccgcg ccgaggtgaa gttcgagggc gacaccctgg tgaaccgcat
      841 cgagctgaag ggcatcgact tcaaggagga cggcaacatc ctggggcaca agctggagta
      901 caacatgaac gtgtgggacg cgtatatcac ggccgacaag cagaagaacg gcatcaaagc
      961 gaacttcaag atcgagcaca acgtcgagga cggcggcgtg cagctcgccg acgcgtacca
     1021 gcagaacacc cccatcggcg acggctccgt gctgctgcct gacaaccact acctgagctt
     1081 ccagagcaag ctgttcaaag accccaacga gcagcgcgat cacatggtcc tgctggagtt
     1141 cgttaccgcc gccgggatca ctcccggcat ggacgagctg tacaagtaag cggccgcgac
     1201 tctagatcat aatcagccat accac
//
