LOCUS       sequence-87436-e        2556 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Nuclear Envelope, Excitation: 406, Emission: 526.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2556)
  TITLE     Davidson Yellow fluorescent proteins
REFERENCE   2  (bases 1 to 2556)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: mAmetrine-LaminB1-10
FEATURES             Location/Qualifiers
     source          1..2556
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      28..37
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(34..36,37..39,40..750)
                     /codon_start=1
                     /product="yellow fluorescent protein with a large Stokes 
                     shift, excitable with violet light (Ai et al., 2008)"
                     /label=mAmetrine
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVRGEGEGDATNGKLTL
                     KFICTSGKLPVPWPTLVTTLSYGVQCFARYPDHMKQHDFFKSAMPEGYVQERTISFKDD
                     GSYRTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNMNVWDAYITADKQKNGIK
                     ANFKIEHNVEDGGVQLADAYQQNTPIGDGSVLLPDNHYLSFQSKLFKDPNEQRDHMVLL
                     EFVTAAGITPGMDELYK"
     primer_bind     complement(79..100)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
ORIGIN
        1 acgtcagatc cgctagcgct accggtcgcc accatggtga gcaagggcga ggagctgttc
       61 accggggtgg tgcccatcct ggtcgagctg gacggcgacg taaacggcca caagttcagc
      121 gtgcgcggcg agggcgaggg cgatgccacc aacggcaagc tgaccctgaa gttcatctgc
      181 acctccggca agctgcccgt gccctggccc accctcgtga ccaccctgtc ttacggcgtg
      241 cagtgcttcg cccgctaccc cgaccacatg aagcagcacg acttcttcaa gtccgccatg
      301 cccgaaggct acgtccagga gcgcaccatc tccttcaagg acgacggcag ctacaggacc
      361 cgcgccgagg tgaagttcga gggcgacacc ctggtgaacc gcatcgagct gaagggcatc
      421 gacttcaagg aggacggcaa catcctgggg cacaagctgg agtacaacat gaacgtgtgg
      481 gacgcgtata tcacggccga caagcagaag aacggcatca aagcgaactt caagatcgag
      541 cacaacgtcg aggacggcgg cgtgcagctc gccgacgcgt accagcagaa cacccccatc
      601 ggcgacggct ccgtgctgct gcctgacaac cactacctga gcttccagag caagctgttc
      661 aaagacccca acgagcagcg cgatcacatg gtcctgctgg agttcgttac cgccgccggg
      721 atcactcccg gcatggacga gctgtacaag tccggactca gatctcgagc tcaagcttcg
      781 atggcgactg cgacccccgt gccgccgcgg atgggcagcc gcgctggcgg ccccaccacg
      841 ccgctgagcc ccacgcgcct gtcgcggctc caggagaagg aggagctgcg cgagctcaat
      901 gaccggctgg cggtgtacat cgacaaggtg cgcagcctgg agacggagaa cagcgcgctg
      961 cagctgcagg tgacggagcg cgaggaggtg cgcggccgtg agctcaccgg cctcaaggcg
     1021 ctctacgaga ccgagctggc cgacgcgcga cgcgcgctcg acgacacggc ccgcgagcgc
     1081 gccaagctgc agatcgagct gggcaagtgc aaggcggaac acgaccagct gctcctcaac
     1141 tatgctaaga aggaatctga tcttaatggc gcccagatca agcttcgaga atatgaagca
     1201 gcactcaatt cgaaagatgc agctcttgct actgcacttg gtgacaaaaa aagtttagag
     1261 ggagatttgg aggatctgaa ggatcagatt gcccagttgg aagcctcctt agctgcagcc
     1321 aaaaaacagt tagcagatga aactttactt aaagtagatt tggagaatcg ttgtcagagc
     1381 cttactgagg acttggagtt tcgcaaaagc atgtatgaag aggagattaa cgagaccaga
     1441 aggaagcatg aaacgcgctt ggtagaggtg gattctgggc gtcaaattga gtatgagtac
     1501 aagctggcgc aagcccttca tgagatgaga gagcaacatg atgcccaagt gaggctgtat
     1561 aaggaggagc tggagcagac ttaccatgcc aaacttgaga atgccagact gtcatcagag
     1621 atgaatactt ctactgtcaa cagtgccagg gaagaactga tggaaagccg catgagaatt
     1681 gagagccttt catcccagct ttctaatcta cagaaagagt ctagagcatg tttggaaagg
     1741 attcaagaat tagaggactt gcttgctaaa gaaaaagaca actctcgtcg catgctgaca
     1801 gacaaagaga gagagatggc ggaaataagg gatcaaatgc agcaacagct gaatgactat
     1861 gaacagcttc ttgatgtaaa gttagccctg gacatggaaa tcagtgctta caggaaactc
     1921 ttagaaggcg aagaagagag gttgaagctg tctccaagcc cttcttcccg tgtgacagta
     1981 tcccgagcat cctcaagtcg tagtgtacgt acaactagag gaaagcggaa gagggttgat
     2041 gtggaagaat cagaggcgag tagtagtgtt agcatctctc attccgcctc agccactgga
     2101 aatgtttgca tcgaagaaat tgatgttgat gggaaattta tccgcttgaa gaacacttct
     2161 gaacaggatc aaccaatggg aggctgggag atgatcagaa aaattggaga cacatcagtc
     2221 agttataaat atacctcaag atatgtgctg aaggcaggcc agactgttac aatttgggct
     2281 gcaaacgctg gtgtcacagc cagcccccca actgacctca tctggaagaa ccagaactcg
     2341 tggggcactg gcgaagatgt gaaggttata ttgaaaaatt ctcagggaga ggaggttgct
     2401 caaagaagta cagtctttaa aacaaccata cctgaagaag aggaggagga ggaagaagca
     2461 gctggagtgg ttgttgagga agaacttttc caccagcagg gaaccccaag agcatccaat
     2521 agaagctgtg caattatgta gggatccacc ggatct
//
