LOCUS       sequence-87438-e        1225 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Cytoskeleton, Excitation: 406, Emission: 526.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1225)
  TITLE     Davidson Yellow fluorescent proteins
REFERENCE   2  (bases 1 to 1225)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: mAmetrine-Profilin-10
FEATURES             Location/Qualifiers
     source          1..1225
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      28..37
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(34..36,37..39,40..750)
                     /codon_start=1
                     /product="yellow fluorescent protein with a large Stokes 
                     shift, excitable with violet light (Ai et al., 2008)"
                     /label=mAmetrine
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVRGEGEGDATNGKLTL
                     KFICTSGKLPVPWPTLVTTLSYGVQCFARYPDHMKQHDFFKSAMPEGYVQERTISFKDD
                     GSYRTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNMNVWDAYITADKQKNGIK
                     ANFKIEHNVEDGGVQLADAYQQNTPIGDGSVLLPDNHYLSFQSKLFKDPNEQRDHMVLL
                     EFVTAAGITPGMDELYK"
     primer_bind     complement(79..100)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
ORIGIN
        1 acgtcagatc cgctagcgct accggtcgcc accatggtga gcaagggcga ggagctgttc
       61 accggggtgg tgcccatcct ggtcgagctg gacggcgacg taaacggcca caagttcagc
      121 gtgcgcggcg agggcgaggg cgatgccacc aacggcaagc tgaccctgaa gttcatctgc
      181 acctccggca agctgcccgt gccctggccc accctcgtga ccaccctgtc ttacggcgtg
      241 cagtgcttcg cccgctaccc cgaccacatg aagcagcacg acttcttcaa gtccgccatg
      301 cccgaaggct acgtccagga gcgcaccatc tccttcaagg acgacggcag ctacaggacc
      361 cgcgccgagg tgaagttcga gggcgacacc ctggtgaacc gcatcgagct gaagggcatc
      421 gacttcaagg aggacggcaa catcctgggg cacaagctgg agtacaacat gaacgtgtgg
      481 gacgcgtata tcacggccga caagcagaag aacggcatca aagcgaactt caagatcgag
      541 cacaacgtcg aggacggcgg cgtgcagctc gccgacgcgt accagcagaa cacccccatc
      601 ggcgacggct ccgtgctgct gcctgacaac cactacctga gcttccagag caagctgttc
      661 aaagacccca acgagcagcg cgatcacatg gtcctgctgg agttcgttac cgccgccggg
      721 atcactcccg gcatggacga gctgtacaag tccggactca gatctcgagc tcaagcttcc
      781 atggccgggt ggaacgccta catcgacagc cttatggcgg acgggacctg tcaggacgcg
      841 gccatcgtag gctacaagga ctcgccctcc gtctgggccg ccgtccccgg gaagaccttc
      901 gttagcatta cgccagctga ggttggtgtc ctggtaggca aagaccggtc aagttttttc
      961 gtcaatgggc tgacacttgg gggccagaaa tgttctgtga tccgggactc actgctgcaa
     1021 gacggggaat ttacaatgga tcttcgtacc aagagcaccg gaggagcccc caccttcaat
     1081 gtcactgtca ccatgactgc caagacgcta gtcctgctga tgggcaaaga aggtgtccac
     1141 ggtggtttga tcaacaagaa atgttatgaa atggcctctc acctgcggcg ttcccagtac
     1201 taaggatcca ccggatctag ataac
//
