LOCUS       sequence-87441-e        2540 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Focal Adhesions, Excitation: 406, Emission: 526.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2540)
  TITLE     Davidson Yellow fluorescent proteins
REFERENCE   2  (bases 1 to 2540)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: mAmetrine-Zyxin-6
FEATURES             Location/Qualifiers
     source          1..2540
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      49..58
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     regulatory      1779..1788
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(1785..1787,1788..1790,1791..2504)
                     /codon_start=1
                     /product="yellow fluorescent protein with a large Stokes 
                     shift, excitable with violet light (Ai et al., 2008)"
                     /label=mAmetrine
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVRGEGEGDATNGKLTL
                     KFICTSGKLPVPWPTLVTTLSYGVQCFARYPDHMKQHDFFKSAMPEGYVQERTISFKDD
                     GSYRTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNMNVWDAYITADKQKNGIK
                     ANFKIEHNVEDGGVQLADAYQQNTPIGDGSVLLPDNHYLSFQSKLFKDPNEQRDHMVLL
                     EFVTAAGITPGMDELYK"
     primer_bind     complement(1830..1851)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
ORIGIN
        1 gatccgctag cgctaccgga ctcagatctc gagctcaagc ttcgaattcc atggcggccc
       61 cccgcccgtc tcccgcgatc cccgtttcgg tctcggctcc ggctttttac gccccgcaga
      121 agaagttcgg ccctgtggtg gccccaaagc ccaaagtgaa tcccttccgg cccagggaca
      181 gcgagcctcc cccggcaccc ggggcccagc gcgcacagat gggccgggtg ggcgatattc
      241 ccccgccgcc cccggaagac tttcccctgc ctccacctcc ccttgctggg gatggcgacg
      301 atgcagaggg tgctctggga ggtgccttcc cgccgccccc tcccccgatc gaggaatcat
      361 ttccccctgc gcctctggag gaggagatct tcccttcccc gccgcctcct ccggaggagg
      421 agggagggcc tgaggccccc ataccgcccc caccacagcc cagggagaag gtgagcagta
      481 ttgatttgga gatcgactct ctgtcctcac tgctggatga catgaccaag aatgatcctt
      541 tcaaagcccg ggtgtcatct ggatatgtgc ccccaccagt ggccactcca ttcagttcca
      601 agtccagtac caagcctgca gccgggggca cagcacccct gcctccttgg aagtcccctt
      661 ccagctccca gcctctgccc caggttccgg ctccggctca gagccagaca cagttccatg
      721 ttcagcccca gccccagccc aagcctcagg tccaactcca tgtccagtcc cagacccagc
      781 ctgtgtcttt ggctaacacc cagccccgag ggcccccagc ctcatctccg gctccagccc
      841 ctaagttttc tccagtgact cctaagttta ctcctgtggc ttccaagttc agtcctggag
      901 ccccaggtgg atctgggtca caaccaaatc aaaaattggg gcaccccgaa gctctttctg
      961 ctggcacagg ctcccctcaa cctcccagct tcacctatgc ccagcagagg gagaagcccc
     1021 gagtgcagga gaagcagcac cccgtgcccc caccggctca gaaccaaaac caggtgcgct
     1081 cccctggggc cccagggccc ctgactctga aggaggtgga ggagctggag cagctgaccc
     1141 agcagctaat gcaggacatg gagcatcctc agaggcagaa tgtggctgtc aacgaactct
     1201 gcggccgatg ccatcaaccc ctggcccggg cgcagccagc cgtccgcgct ctagggcagc
     1261 tgttccacat cgcctgcttc acctgccacc agtgtgcgca gcagctccag ggccagcagt
     1321 tctacagtct ggagggggcg ccgtactgcg agggctgtta cactgacacc ctggagaagt
     1381 gtaacacctg cggggagccc atcactgacc gcatgctgag ggccacgggc aaggcctatc
     1441 acccgcactg cttcacctgt gtggtctgcg cccgccccct ggagggcacc tccttcatcg
     1501 tggaccaggc caaccggccc cactgtgtcc ccgactacca caagcagtac gccccgaggt
     1561 gctccgtctg ctctgagccc atcatgcctg agcctggccg agatgagact gtgcgagtgg
     1621 tcgccctgga caagaacttc cacatgaagt gttacaagtg tgaggactgc gggaagcccc
     1681 tgtcgattga ggcagatgac aatggctgct tccccctgga cggtcacgtg ctctgtcgga
     1741 agtgccacac tgctagagcc cagacggatc caccggtcgc caccatggtg agcaagggcg
     1801 aggagctgtt caccggggtg gtgcccatcc tggtcgagct ggacggcgac gtaaacggcc
     1861 acaagttcag cgtgcgcggc gagggcgagg gcgatgccac caacggcaag ctgaccctga
     1921 agttcatctg cacctccggc aagctgcccg tgccctggcc caccctcgtg accaccctgt
     1981 cttacggcgt gcagtgcttc gcccgctacc ccgaccacat gaagcagcac gacttcttca
     2041 agtccgccat gcccgaaggc tacgtccagg agcgcaccat ctccttcaag gacgacggca
     2101 gctacaggac ccgcgccgag gtgaagttcg agggcgacac cctggtgaac cgcatcgagc
     2161 tgaagggcat cgacttcaag gaggacggca acatcctggg gcacaagctg gagtacaaca
     2221 tgaacgtgtg ggacgcgtat atcacggccg acaagcagaa gaacggcatc aaagcgaact
     2281 tcaagatcga gcacaacgtc gaggacggcg gcgtgcagct cgccgacgcg taccagcaga
     2341 acacccccat cggcgacggc tccgtgctgc tgcctgacaa ccactacctg agcttccaga
     2401 gcaagctgtt caaagacccc aacgagcagc gcgatcacat ggtcctgctg gagttcgtta
     2461 ccgccgccgg gatcactccc ggcatggacg agctgtacaa gtaagcggcc gcgactctag
     2521 atcataatca gccataccac
//
