LOCUS       sequence-87442-e        1931 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Actin, Excitation: 516, Emission: 529.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1931)
  TITLE     Davidson Yellow fluorescent proteins
REFERENCE   2  (bases 1 to 1931)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: Citrine-Actin-3
FEATURES             Location/Qualifiers
     source          1..1931
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             join(31..33,34..36,37..747)
                     /codon_start=1
                     /product="enhanced variant of YFP (Heikal et al., 2001)"
                     /label=Citrine
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTFGYGLMCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSYQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(76..97)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(337..356)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     684..705
                     /label=EGFP-C
                     /note="EGFP, forward primer"
ORIGIN
        1 gacgtcagat ccgctagcgc taccggtacc atggtgagca agggcgagga gctgttcacc
       61 ggggtggtgc ccatcctggt cgagctggac ggcgacgtaa acggccacaa gttcagcgtg
      121 tccggcgagg gcgagggcga tgccacctac ggcaagctga ccctgaagtt catctgcacc
      181 accggcaagc tgcccgtgcc ctggcccacc ctcgtgacca ccttcggcta cggcctgatg
      241 tgcttcgccc gctaccccga ccacatgaag cagcacgact tcttcaagtc cgccatgccc
      301 gaaggctacg tccaggagcg caccatcttc ttcaaggacg acggcaacta caagacccgc
      361 gccgaggtga agttcgaggg cgacaccctg gtgaaccgca tcgagctgaa gggcatcgac
      421 ttcaaggagg acggcaacat cctggggcac aagctggagt acaactacaa cagccacaac
      481 gtctatatca tggccgacaa gcagaagaac ggcatcaagg tgaacttcaa gatccgccac
      541 aacatcgagg acggcagcgt gcagctcgcc gaccactacc agcagaacac ccccatcggc
      601 gacggccccg tgctgctgcc cgacaaccac tacctgagct accagtccgc cctgagcaaa
      661 gaccccaacg agaagcgcga tcacatggtc ctgctggagt tcgtgaccgc cgccgggatc
      721 actctcggca tggacgagct gtacaagtct cgagctatgg atgatgatat cgccgcgctc
      781 gtcgtcgaca acggctccgg catgtgcaag gccggcttcg cgggcgacga tgccccccgg
      841 gccgtcttcc cctccatcgt ggggcgcccc aggcaccagg gcgtgatggt gggcatgggt
      901 cagaaggatt cctatgtggg cgacgaggcc cagagcaaga gaggcatcct caccctgaag
      961 taccccatcg agcacggcat cgtcaccaac tgggacgaca tggagaaaat ctggcaccac
     1021 accttctaca atgagctgcg tgtggctccc gaggagcacc ccgtgctgct gaccgaggcc
     1081 cccctgaacc ccaaggccaa ccgcgagaag atgacccaga tcatgtttga gaccttcaac
     1141 accccagcca tgtacgttgc tatccaggct gtgctatccc tgtacgcctc tggccgtacc
     1201 actggcatcg tgatggactc cggtgacggg gtcacccaca ctgtgcccat ctacgagggg
     1261 tatgccctcc cccatgccat cctgcgtctg gacctggctg gccgggacct gactgactac
     1321 ctcatgaaga tcctcaccga gcgcggctac agcttcacca ccacggccga gcgggaaatc
     1381 gtgcgtgaca ttaaggagaa gctgtgctac gtcgccctgg acttcgagca agagatggcc
     1441 acggctgctt ccagctcctc cctggagaag agctacgagc tgcctgacgg ccaggtcatc
     1501 accattggca atgagcggtt ccgctgccct gaggcactct tccagccttc cttcctgggc
     1561 atggagtcct gtggcatcca cgaaactacc ttcaactcca tcatgaagtg tgacgtggac
     1621 atccgcaaag acctgtacgc caacacagtg ctgtctggcg gcaccaccat gtaccctggc
     1681 attgccgaca ggatgcagaa ggagatcact gccctggcac ccagcacaat gaagatcaag
     1741 atcattgctc ctcctgagcg caagtactcc gtgtggatcg gcggctccat cctggcctcg
     1801 ctgtccacct tccagcagat gtggatcagc aagcaggagt atgacgagtc cggcccctcc
     1861 atcgtccacc gcaaatgctt ctagggatcc accggatcta gataactgat cataatcagc
     1921 cataccacag t
//
