LOCUS       sequence-87448-e        1775 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Nucleoli, Excitation: 516, Emission: 529.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1775)
  TITLE     Davidson Yellow fluorescent proteins
REFERENCE   2  (bases 1 to 1775)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: mCitrine-Fibrillarin-7
FEATURES             Location/Qualifiers
     source          1..1775
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      30..39
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(36..38,39..41,42..752)
                     /codon_start=1
                     /product="enhanced YFP with monomerizing A206K mutation 
                     (Zacharias et al., 2002)"
                     /label=mEYFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTFGYGLMCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSYQSKLSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(81..102)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(342..361)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     689..710
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     CDS             complement(1548..1559)
                     /codon_start=1
                     /gene="cspA"
                     /product="translation enhancing element for E. coli (Qing 
                     et al., 2004)"
                     /label=TEE
                     /translation="NHKV"
ORIGIN
        1 tgacgtcaga tccgctagcg ctaccggtcg ccaccatggt gagcaagggc gaggagctgt
       61 tcaccggggt ggtgcccatc ctggtcgagc tggacggcga cgtaaacggc cacaagttca
      121 gcgtgtccgg cgagggcgag ggcgatgcca cctacggcaa gctgaccctg aagttcatct
      181 gcaccaccgg caagctgccc gtgccctggc ccaccctcgt gaccaccttc ggctacggcc
      241 tgatgtgctt cgcccgctac cccgaccaca tgaagcagca cgacttcttc aagtccgcca
      301 tgcccgaagg ctacgtccag gagcgcacca tcttcttcaa ggacgacggc aactacaaga
      361 cccgcgccga ggtgaagttc gagggcgaca ccctggtgaa ccgcatcgag ctgaagggca
      421 tcgacttcaa ggaggacggc aacatcctgg ggcacaagct ggagtacaac tacaacagcc
      481 acaacgtcta tatcatggcc gacaagcaga agaacggcat caaggtgaac ttcaagatcc
      541 gccacaacat cgaggacggc agcgtgcagc tcgccgacca ctaccagcag aacaccccca
      601 tcggcgacgg ccccgtgctg ctgcccgaca accactacct gagctaccag tccaagctga
      661 gcaaagaccc caacgagaag cgcgatcaca tggtcctgct ggagttcgtg accgccgccg
      721 ggatcactct cggcatggac gagctgtaca agtccggact cagatctcga gccatgaagc
      781 caggattcag tccccgtggg ggtggctttg gcggccgagg gggctttggt gaccgtggtg
      841 gtcgtggagg ccgagggggc tttggcgggg gccgaggcca aggcggaggc tttagaggtc
      901 gtggacgagg aggaggtgga ggcggcggcg gcggtggagg aggaggaaga ggtggtggag
      961 gcttccattt tggtggcaac cggggtcgtg gtcggggagg aaaaagagga aaccagtcgg
     1021 ggaagaatgt gatggtggag ccgcatcggc atgagggtgt cttcatttgt cggggaaagg
     1081 aagatgcact ggtcaccaag aacctggtcc ctggggaatc agtttatgga gagaagagag
     1141 tctcgatttc ggaaggagat gacaaaattg agtaccgagc ctggaacccc ttccgctcca
     1201 agcttgcagc agcaatcctg ggtggtgtgg accagatcca catcaaaccg ggggctaagg
     1261 ttctctacct cggggctgcc tcgggcacca cggtctccca tgtctctgac atcgttggtc
     1321 cggatggtct agtctatgca gtcgagttct cccaccgctc tggccgtgac ctcattaact
     1381 tggccaagaa gaggaccaac atcattcctg tgatcgagga tgctcgacac ccacacaaat
     1441 accgcatgct catcgcaatg gtggatgtga tctttgctga tgtggcccag ccagaccaga
     1501 cccggattgt ggccctgaat gcccacacct tcctgcgtaa tggaggacac tttgtgattt
     1561 ccattaaggc caactgcatt gactccacag cctcagccga ggccgtgttt gcctccgaag
     1621 tgaaaaagat gcaacaggag aacatgaagc cgcaggagca gttgaccctt gagccatatg
     1681 aaagagacca tgccgtggtc gtgggagtgt acaggccacc ccccaaggtg aagaactgag
     1741 gatccaccgg atctagataa ctgatcataa tcagc
//
