LOCUS       sequence-87449-e        2187 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Centrosomes, Excitation: 516, Emission: 529.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2187)
  TITLE     Davidson Yellow fluorescent proteins
REFERENCE   2  (bases 1 to 2187)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: mCitrine-Gamma-Tubulin-17
FEATURES             Location/Qualifiers
     source          1..2187
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      1423..1432
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(1429..1431,1432..1434,1435..2148)
                     /codon_start=1
                     /product="enhanced YFP with monomerizing A206K mutation 
                     (Zacharias et al., 2002)"
                     /label=mEYFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTFGYGLMCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSYQSKLSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(1474..1495)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(1735..1754)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     2082..2103
                     /label=EGFP-C
                     /note="EGFP, forward primer"
ORIGIN
        1 gacgtcagat ccgctagcgc caccatgccg agggaaatca tcaccctaca gttgggccag
       61 tgcggcaatc agattgggtt cgagttctgg aaacagctgt gcgccgagca tggtatcagc
      121 cccgagggca tcgtggagga gttcgccacc gagggcactg accacaagga cgtctttttc
      181 taccaggcag acgatgagca ctacatcccc cgggccgtgc tgctggactt ggaaccccgg
      241 gtgatccact ccatcctcaa ctccccctat gccaagctct acaacccaga gaacatctac
      301 ctgtcggaac atggaggagg agctggcaac aactgggcca gcggattctc ccagggagaa
      361 aagatccatg aggacatttt tgacatcata gaccgggagg cagatggtag tgacagtcta
      421 gagggctttg tgctgtgtca ctccattgct ggggggacag gctctggact gggttcctac
      481 ctcttagaac ggctgaatga caggtatcct aagaagctgg tgcagacata ctcagtgttt
      541 cccaaccagg acgagatgag cgatgtggtg gtccagcctt acaattcact cctcacactc
      601 aagaggctga cgcagaatgc agactgtgtg gtggtgctgg acaacacagc cctgaaccgg
      661 attgccacag accgcctgca catccagaac ccatccttct cccagatcaa ccagctggtg
      721 tctaccatca tgtcagccag caccaccacc ctgcgctacc ctggctacat gaacaatgac
      781 ctcatcggcc tcatcgcctc gctcattccc accccacggc tccacttcct catgaccggc
      841 tacacccctc tcactacgga ccagtcagtg gccagcgtga ggaagaccac ggtcctggat
      901 gtcatgaggc ggctgctgca gcccaagaac gtgatggtgt ccacaggccg agaccgccag
      961 accaaccact gctacatcgc catcctcaac atcatccagg gagaggtgga ccccacccag
     1021 gtccacaaga gcttgcagag gatccgggaa cgcaagttgg ccaacttcat cccgtggggc
     1081 cccgccagca tccaggtggc cctgtcgagg aagtctccct acctgccctc ggcccaccgg
     1141 gtcagcgggc tcatgatggc caaccacacc agcatctcct cgctcttcga gagaacctgt
     1201 cgccagtatg acaagctgcg taagcgggag gccttcctgg agcagttccg caaggaggac
     1261 atgttcaagg acaactttga tgagatggac acatccaggg agattgtgca gcagctcatc
     1321 gatgagtacc atgcggccac acggccagac tacatctcct ggggcactca ggagcagcga
     1381 attctgcagt cgacggtacc gcgggcccgg gatccaccgg tcgccaccat ggtgagcaag
     1441 ggcgaggagc tgttcaccgg ggtggtgccc atcctggtcg agctggacgg cgacgtaaac
     1501 ggccacaagt tcagcgtgtc cggcgagggc gagggcgatg ccacctacgg caagctgacc
     1561 ctgaagttca tctgcaccac cggcaagctg cccgtgccct ggcccaccct cgtgaccacc
     1621 ttcggctacg gcctgatgtg cttcgcccgc taccccgacc acatgaagca gcacgacttc
     1681 ttcaagtccg ccatgcccga aggctacgtc caggagcgca ccatcttctt caaggacgac
     1741 ggcaactaca agacccgcgc cgaggtgaag ttcgagggcg acaccctggt gaaccgcatc
     1801 gagctgaagg gcatcgactt caaggaggac ggcaacatcc tggggcacaa gctggagtac
     1861 aactacaaca gccacaacgt ctatatcatg gccgacaagc agaagaacgg catcaaggtg
     1921 aacttcaaga tccgccacaa catcgaggac ggcagcgtgc agctcgccga ccactaccag
     1981 cagaacaccc ccatcggcga cggccccgtg ctgctgcccg acaaccacta cctgagctac
     2041 cagtccaagc tgagcaaaga ccccaacgag aagcgcgatc acatggtcct gctggagttc
     2101 gtgaccgccg ccgggatcac tctcggcatg gacgagctgt acaagtaagc ggccgcgact
     2161 ctagatcata atcagccata ccacatt
//
