LOCUS       sequence-87452-e        1049 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Mitochondria, Excitation: 516, Emission: 529.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1049)
  TITLE     Davidson Yellow fluorescent proteins
REFERENCE   2  (bases 1 to 1049)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: mCitrine-Mito-7
FEATURES             Location/Qualifiers
     source          1..1049
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        35..86
                     /label=CMVd2 promoter
                     /note="truncated human cytomegalovirus (CMV) immediate 
                     early promoter that yields moderately reduced expression 
                     relative to the full-length promoter"
     primer_bind     37..57
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     CDS             115..189
                     /codon_start=1
                     /product="mitochondrial presequence of human cytochrome c 
                     oxidase subunit VIII (Rizutto et al., 1989)"
                     /label=COX8 presequence
                     /translation="MSVLTPLLLRGLTGSARRLPVPRAK"
     regulatory      217..226
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(223..225,226..228,229..942)
                     /codon_start=1
                     /product="enhanced YFP with monomerizing A206K mutation 
                     (Zacharias et al., 2002)"
                     /label=mEYFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTFGYGLMCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSYQSKLSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(268..289)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(529..548)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     876..897
                     /label=EGFP-C
                     /note="EGFP, forward primer"
ORIGIN
        1 actttccaaa atgtcgtaac aactccgccc cattgacgca aatgggcggt aggcgtgtac
       61 ggtgggaggt ctatataagc agagctggtt tagtgaaccg tcagatccgc tagcatgtcc
      121 gtcctgacgc cgctgctgct gcggggcttg acaggctcgg cccggcggct cccagtgccg
      181 cgcgccaaga tccattcgtt gggggatcca ccggtcgcca ccatggtgag caagggcgag
      241 gagctgttca ccggggtggt gcccatcctg gtcgagctgg acggcgacgt aaacggccac
      301 aagttcagcg tgtccggcga gggcgagggc gatgccacct acggcaagct gaccctgaag
      361 ttcatctgca ccaccggcaa gctgcccgtg ccctggccca ccctcgtgac caccttcggc
      421 tacggcctga tgtgcttcgc ccgctacccc gaccacatga agcagcacga cttcttcaag
      481 tccgccatgc ccgaaggcta cgtccaggag cgcaccatct tcttcaagga cgacggcaac
      541 tacaagaccc gcgccgaggt gaagttcgag ggcgacaccc tggtgaaccg catcgagctg
      601 aagggcatcg acttcaagga ggacggcaac atcctggggc acaagctgga gtacaactac
      661 aacagccaca acgtctatat catggccgac aagcagaaga acggcatcaa ggtgaacttc
      721 aagatccgcc acaacatcga ggacggcagc gtgcagctcg ccgaccacta ccagcagaac
      781 acccccatcg gcgacggccc cgtgctgctg cccgacaacc actacctgag ctaccagtcc
      841 aagctgagca aagaccccaa cgagaagcgc gatcacatgg tcctgctgga gttcgtgacc
      901 gccgccggga tcactctcgg catggacgag ctgtacaagt aagcggccgc gactctagat
      961 cataatcagc cataccacat ttgtagaggt tttacttgct ttaaaaaacc tcccacacct
     1021 ccccctgaac ctgaacataa aatgaatgc
//
