LOCUS       sequence-87454-e        1474 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Ras GTPase, Excitation: 516, Emission: 529.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1474)
  TITLE     Davidson Yellow fluorescent proteins
REFERENCE   2  (bases 1 to 1474)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: mCitrine-Rab4a-7
FEATURES             Location/Qualifiers
     source          1..1474
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      29..38
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(35..37,38..40,41..751)
                     /codon_start=1
                     /product="enhanced YFP with monomerizing A206K mutation 
                     (Zacharias et al., 2002)"
                     /label=mEYFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTFGYGLMCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSYQSKLSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(80..101)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(341..360)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     688..709
                     /label=EGFP-C
                     /note="EGFP, forward primer"
ORIGIN
        1 gacgtcagat ccgctagcgc taccggtcgc caccatggtg agcaagggcg aggagctgtt
       61 caccggggtg gtgcccatcc tggtcgagct ggacggcgac gtaaacggcc acaagttcag
      121 cgtgtccggc gagggcgagg gcgatgccac ctacggcaag ctgaccctga agttcatctg
      181 caccaccggc aagctgcccg tgccctggcc caccctcgtg accaccttcg gctacggcct
      241 gatgtgcttc gcccgctacc ccgaccacat gaagcagcac gacttcttca agtccgccat
      301 gcccgaaggc tacgtccagg agcgcaccat cttcttcaag gacgacggca actacaagac
      361 ccgcgccgag gtgaagttcg agggcgacac cctggtgaac cgcatcgagc tgaagggcat
      421 cgacttcaag gaggacggca acatcctggg gcacaagctg gagtacaact acaacagcca
      481 caacgtctat atcatggccg acaagcagaa gaacggcatc aaggtgaact tcaagatccg
      541 ccacaacatc gaggacggca gcgtgcagct cgccgaccac taccagcaga acacccccat
      601 cggcgacggc cccgtgctgc tgcccgacaa ccactacctg agctaccagt ccaagctgag
      661 caaagacccc aacgagaagc gcgatcacat ggtcctgctg gagttcgtga ccgccgccgg
      721 gatcactctc ggcatggacg agctgtacaa gtccggactc agatctcgag ccatgtcgca
      781 gacggccatg tccgaaacct acgatttttt gtttaagttc ttggttattg gaaatgcagg
      841 aactggcaaa tcttgcttac ttcatcagtt tattgaaaaa aaattcaaag atgactcaaa
      901 tcatacaata ggagtggaat ttggttcaaa gataataaat gttggtggta aatatgtaaa
      961 gttacaaata tgggatacag caggacaaga acgattcagg tccgtgacga gaagttatta
     1021 ccgaggcgcg gccggggctc tcctcgtcta tgatatcacc agccgagaaa cctacaatgc
     1081 gcttactaat tggttaacag atgcccgaat gctggcgagc cagaacattg tgatcatcct
     1141 ttgtggaaac aagaaggacc tggatgcaga tcgtgaagtt accttcttag aagcctccag
     1201 atttgctcaa gaaaatgagc tgatgttttt ggaaacaagt gcgctcacag gggagaatgt
     1261 agaagaggct tttgtacagt gtgcaagaaa aatacttaac aaaatcgaat caggtgagct
     1321 ggacccagaa agaatgggct caggtattca gtacggagat gctgccttga gacagctgag
     1381 gtcaccgcgg cgcgcacagg ccccgaacgc tcaggagtgt ggttgttagg gatccaccgg
     1441 atctagataa ctgatcataa tcagccatac caca
//
