LOCUS       sequence-87456-e        1439 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Ras GTPase, Excitation: 516, Emission: 529.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1439)
  TITLE     Davidson Yellow fluorescent proteins
REFERENCE   2  (bases 1 to 1439)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: mCitrine-Rab7a-7
FEATURES             Location/Qualifiers
     source          1..1439
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      28..37
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(34..36,37..39,40..750)
                     /codon_start=1
                     /product="enhanced YFP with monomerizing A206K mutation 
                     (Zacharias et al., 2002)"
                     /label=mEYFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTFGYGLMCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSYQSKLSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(79..100)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(340..359)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     687..708
                     /label=EGFP-C
                     /note="EGFP, forward primer"
ORIGIN
        1 acgtcagatc cgctagcgct accggtcgcc accatggtga gcaagggcga ggagctgttc
       61 accggggtgg tgcccatcct ggtcgagctg gacggcgacg taaacggcca caagttcagc
      121 gtgtccggcg agggcgaggg cgatgccacc tacggcaagc tgaccctgaa gttcatctgc
      181 accaccggca agctgcccgt gccctggccc accctcgtga ccaccttcgg ctacggcctg
      241 atgtgcttcg cccgctaccc cgaccacatg aagcagcacg acttcttcaa gtccgccatg
      301 cccgaaggct acgtccagga gcgcaccatc ttcttcaagg acgacggcaa ctacaagacc
      361 cgcgccgagg tgaagttcga gggcgacacc ctggtgaacc gcatcgagct gaagggcatc
      421 gacttcaagg aggacggcaa catcctgggg cacaagctgg agtacaacta caacagccac
      481 aacgtctata tcatggccga caagcagaag aacggcatca aggtgaactt caagatccgc
      541 cacaacatcg aggacggcag cgtgcagctc gccgaccact accagcagaa cacccccatc
      601 ggcgacggcc ccgtgctgct gcccgacaac cactacctga gctaccagtc caagctgagc
      661 aaagacccca acgagaagcg cgatcacatg gtcctgctgg agttcgtgac cgccgccggg
      721 atcactctcg gcatggacga gctgtacaag tccggactca gatctcgagc catgacctct
      781 aggaagaaag tgttgctgaa ggttatcatc ctgggagatt ctggagtcgg gaagacatca
      841 ctcatgaacc agtatgtgaa taagaaattc agcaatcagt acaaagccac aataggagct
      901 gactttctga ccaaggaggt gatggtggat gacaggctag tcacaatgca gatatgggac
      961 acagcaggac aggaacggtt ccagtctctc ggtgtggcct tctacagagg tgcagactgc
     1021 tgcgttctgg tatttgatgt gactgccccc aacacattca aaaccctaga tagctggaga
     1081 gatgagtttc tcatccaggc cagtccccga gatcctgaaa acttcccatt tgttgtgttg
     1141 ggaaacaaga ttgacctcga aaacagacaa gtggccacaa agcgggcaca ggcctggtgc
     1201 tacagcaaaa acaacattcc ctactttgag accagtgcca aggaggccat caacgtggag
     1261 caggcgttcc agacgattgc acggaatgca cttaagcagg aaacggaggt ggagctgtac
     1321 aacgaatttc ctgaacctat caaactggac aagaatgacc gggccaaggc ctcggcagaa
     1381 agctgcagtt gctgaggatc caccggatct agataactga tcataatcag ccataccac
//
