LOCUS       sequence-87458-e        1401 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Talin Actin Binding Domain, Excitation: 516, Emission:
            529.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1401)
  TITLE     Davidson Yellow fluorescent proteins
REFERENCE   2  (bases 1 to 1401)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: mCitrine-Talin-ABM-10
FEATURES             Location/Qualifiers
     source          1..1401
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      18..27
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(24..26,27..29,30..740)
                     /codon_start=1
                     /product="enhanced YFP with monomerizing A206K mutation 
                     (Zacharias et al., 2002)"
                     /label=mEYFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTFGYGLMCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSYQSKLSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(69..90)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(330..349)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     677..698
                     /label=EGFP-C
                     /note="EGFP, forward primer"
ORIGIN
        1 cgctagcgct accggtcgcc accatggtga gcaagggcga ggagctgttc accggggtgg
       61 tgcccatcct ggtcgagctg gacggcgacg taaacggcca caagttcagc gtgtccggcg
      121 agggcgaggg cgatgccacc tacggcaagc tgaccctgaa gttcatctgc accaccggca
      181 agctgcccgt gccctggccc accctcgtga ccaccttcgg ctacggcctg atgtgcttcg
      241 cccgctaccc cgaccacatg aagcagcacg acttcttcaa gtccgccatg cccgaaggct
      301 acgtccagga gcgcaccatc ttcttcaagg acgacggcaa ctacaagacc cgcgccgagg
      361 tgaagttcga gggcgacacc ctggtgaacc gcatcgagct gaagggcatc gacttcaagg
      421 aggacggcaa catcctgggg cacaagctgg agtacaacta caacagccac aacgtctata
      481 tcatggccga caagcagaag aacggcatca aggtgaactt caagatccgc cacaacatcg
      541 aggacggcag cgtgcagctc gccgaccact accagcagaa cacccccatc ggcgacggcc
      601 ccgtgctgct gcccgacaac cactacctga gctaccagtc caagctgagc aaagacccca
      661 acgagaagcg cgatcacatg gtcctgctgg agttcgtgac cgccgccggg atcactctcg
      721 gcatggacga gctgtacaag tccggactca gatctcgagc tcaagcttcg atcctagaag
      781 ctgccaagtc catcgctgca gccaccagtg cactggtaaa ggctgcgtca gcagcccaga
      841 gggagctggt ggctcaagga aaggtgggcg ccattccagc caatgcactg gatgatgggc
      901 agtggtcgca gggcctcatt tccgctgccc gtatggtggc tgcggccacc aacaatctgt
      961 gtgaagcagc taacgcagct gtccagggcc atgctagcca ggagaaactc atttcctcag
     1021 ccaagcaagt agctgcctcc acagctcagc tcctggtagc ttgcaaggtc aaagcagatc
     1081 aggactctga ggcaatgaaa cggctccagg ctgctggcaa tgcagtgaag agggcctcag
     1141 ataacctggt aaaggcggcc cagaaagctg cagccttcga agaccaggag aatgagacgg
     1201 tggtggtgaa ggagaaaatg gttgggggca ttgcccagat tatcgcagca caggaagaga
     1261 tgcttcggaa ggaacgagag ctggaagagg ctcggaaaaa gctcgcccag atccggcagc
     1321 agcagtacaa gttcttgcct tcagagcttc gagacgagca ctaaggatcc accggatcta
     1381 gataactgat cataatcagc c
//
