LOCUS       sequence-87459-e        7171 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Focal Adhesions, Excitation: 516, Emission: 529.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 7171)
  TITLE     Davidson Yellow fluorescent proteins
REFERENCE   2  (bases 1 to 7171)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: mCitrine-Talin-R-15
FEATURES             Location/Qualifiers
     source          1..7171
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      24..33
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(30..32,33..35,36..746)
                     /codon_start=1
                     /product="enhanced YFP with monomerizing A206K mutation 
                     (Zacharias et al., 2002)"
                     /label=mEYFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTFGYGLMCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSYQSKLSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(75..96)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(336..355)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     683..704
                     /label=EGFP-C
                     /note="EGFP, forward primer"
ORIGIN
        1 cagatccgct agcgctaccg gtcgccacca tggtgagcaa gggcgaggag ctgttcaccg
       61 gggtggtgcc catcctggtc gagctggacg gcgacgtaaa cggccacaag ttcagcgtgt
      121 ccggcgaggg cgagggcgat gccacctacg gcaagctgac cctgaagttc atctgcacca
      181 ccggcaagct gcccgtgccc tggcccaccc tcgtgaccac cttcggctac ggcctgatgt
      241 gcttcgcccg ctaccccgac cacatgaagc agcacgactt cttcaagtcc gccatgcccg
      301 aaggctacgt ccaggagcgc accatcttct tcaaggacga cggcaactac aagacccgcg
      361 ccgaggtgaa gttcgagggc gacaccctgg tgaaccgcat cgagctgaag ggcatcgact
      421 tcaaggagga cggcaacatc ctggggcaca agctggagta caactacaac agccacaacg
      481 tctatatcat ggccgacaag cagaagaacg gcatcaaggt gaacttcaag atccgccaca
      541 acatcgagga cggcagcgtg cagctcgccg accactacca gcagaacacc cccatcggcg
      601 acggccccgt gctgctgccc gacaaccact acctgagcta ccagtccaag ctgagcaaag
      661 accccaacga gaagcgcgat cacatggtcc tgctggagtt cgtgaccgcc gccgggatca
      721 ctctcggcat ggacgagctg tacaagtccg gactcagatc tcgagctcaa gctagcttcg
      781 aattcgccac catgcagcag tacaaccgag tggggaaagt ggagcacggc tctgtggctc
      841 tgccagccat catgcgctct ggagcctctg gtcctgagaa tttccaggtg gggagcatgc
      901 cacctgccca gcagcagatc accagtggcc agatgcaccg aggacacatg ccacctctga
      961 cttcagccca gcaggcgctc actggaacca ttaactccag catgcaggct gtgcaggctg
     1021 cccaggccac tctggatgac tttgagactc taccccctct tggccaggat gctgcctcca
     1081 aagcttggcg taagaacaag atggatgaat caaagcatga gatccactcc caggtagatg
     1141 ccatcacagc tggcactgcc tctgtggtaa acctgacagc aggagatcct gcagagacag
     1201 actatacagc agtgggctgt gcagtcacca ccatctcttc caacctgacg gagatgtccc
     1261 gtggggtgaa gctattggct gccctgctgg aggatgaagg cggcaatggc cggccccttc
     1321 tgcaagcagc aaagggcctt gcaggggctg tgtcggaact gcttcgcagt gctcagcccg
     1381 ccagcgctga gcctcgtcag aacctgctgc aggcagccgg gaacgtgggc caggccagtg
     1441 gggagctgtt gcagcaaatt ggggaaagtg acactgaccc ccacttccag gatgttctaa
     1501 tgcagctagc aaacgcagtg gcaagtgctg cagccgccct ggtcctcaag gccaagagtg
     1561 tggcccagcg cacagaggat tccgggcttc agacccaagt gattgctgca gctacgcaat
     1621 gtgctctgtc cacttcccag ctggtggcct gtaccaaggt ggtagcacct acaatcagct
     1681 cacctgtgtg ccaagaacag ctagtcgagg ctgggcgact ggtggccaaa gctgtggagg
     1741 gctgtgtgtc tgcctcgcaa gcagctacag aagatggaca gctgcttcga ggggtaggag
     1801 cagcagccac ggctgtcacc caggccctca atgagctgct gcagcacgtg aaggcccacg
     1861 ccaccggagc tgggcctgcc ggtcggtatg accaagccac tgataccatc ctcaccgtca
     1921 ctgaaaacat cttcagctcc atgggtgatg ctggggagat ggtgcgccag gcccgcatcc
     1981 tggcccaagc cacctcagac ctggtcaatg ctatcaaggc cgatgctgag ggggagagtg
     2041 atctggagaa ctctagaaag ctcctgagtg ctgccaagat cctcgctgat gccaccgcca
     2101 agatggtgga ggcggccaag ggagcagccg cccaccctga cagtgaggaa cagcagcagc
     2161 gactgcgtga agcagctgag gggcttcgca tggccaccaa tgcagctgcg cagaacgcca
     2221 tcaagaagaa gttggtgcag cgcctggagc atgcagccaa gcaagctgca gcctctgcaa
     2281 cacagaccat tgctgcagcc caacatgcag cctctgcccc caaggcctct gcgggccccc
     2341 agcccctact ggtgcagagc tgtaaggccg tggcagagca gattccgttg ctggtgcagg
     2401 gtgtccgagg aagtcaagct cagcctgaca gccctagtgc tcagctcgcc ctcattgctg
     2461 ccagccagag cttcctgcag ccaggtggga agatggtggc agcggcaaaa gcctcagtgc
     2521 caacaattca ggaccaggct tcagcgatgc agctgagtca gtgcgcaaag aacctaggca
     2581 ctgccctggc cgagctgcgc actgctgctc agaaggctca ggaggcatgt ggacctttgg
     2641 agatggattc tgcactgagt gttgtacaaa atctagagaa agacctacag gaaataaaag
     2701 cagcagctcg agatggcaag cttaagccct tacctgggga aacaatggag aagtgtaccc
     2761 aagatcttgg caacagcacc aaagcggtga gctctgccat cgccaagctg ctgggggaga
     2821 ttgcccaggg caatgagaac tatgcaggta ttgcagctcg ggatgtagct ggtggactaa
     2881 ggtcactagc ccaggctgcg cggggtgtgg ctgcgctgac atcagatcct gcagtgcagg
     2941 ccattgtgct tgacacagcc agcgacgttc tggacaaggc cagcagcctc attgaagagg
     3001 caaaaaaagc atctggacac ccaggggacc cagaaagcca gcaaaggctt gctcaggtag
     3061 ctaaagcagt gacccaagcc ctgaaccgct gtgtcagctg cctgcctggc caaagagacg
     3121 tggataatgc cctacgagcc gttggagatg ccagcaagcg actcctgagt gacttgcttc
     3181 ctccaagcac ggggacattt caagaagcac agagccgatt gaatgaagct gcagctgggt
     3241 taaatcaggc cgccacagaa ctggtgcaag cctcccgagg aactcctcag gacctggctc
     3301 gggcctccgg ccgatttgga caggacttca gcaccttcct ggaagctggt gtggagatgg
     3361 ccggacaggc cccgagccag gaggaccgag cccaggtggt gtccaacctg aagggcatat
     3421 ccatgtcttc aagcaaactt ctccttgccg ctaaggcctt gtccacagac cctgcttctc
     3481 ccaacctcaa gagtcagctg gctgcggctg cccgggcagt gacggacagc atcaaccagc
     3541 tcatcaccat gtgcacccag caggcacctg gccagaagga gtgtgacaat gcacttcggc
     3601 agctagagac agtccgagaa ctcctggaga atccagtcca gcccatcaac gacatgtcct
     3661 acttcggttg cttggacagt gtcatggaga actccaaggt cctaggtgag gccatgactg
     3721 gcatctccca aaatgccaag aatggaaatc tgccggagtt tggagatgcc attgccacag
     3781 cctccaaggc tctctgtggc ttcacggagg cagccgcaca ggcagcatat ctagttggtg
     3841 tctctgaccc caacagccaa gctggacagc aaggactggt ggaacccaca cagtttgccc
     3901 gtgcaaacca ggcaattcag atggcctgtc agagtctggg ggagcctggc tgtacccagg
     3961 cccaggtgtt atctgcagcc actattgtag ccaaacacac atctgcattg tgtaacagct
     4021 gtcgcctggc ttccgctagg actgccaatc ccactgccaa gcgccagttt gtacagtcag
     4081 ccaaggaggt ggccaacagt acagccaatc ttgtcaagac catcaaggca ctagatgggg
     4141 acttcacaga agagaaccgt gcccagtgcc gagcagccac agcccctctg ctggaagctg
     4201 tggataacct gagtgccttt gcctccaacc ctgagttctc cagcgtccct gcccagatca
     4261 gccctgaggg cagagcagcc atggagccca ttgtaatctc tgctaagaca atgttggaga
     4321 gtgctggagg gctcatccag acagctcggg cgttagcagt caatccccga gaccccccac
     4381 gttggtctgt gctagctggc cactctcgga ctgtctcaga ttccatcaag aaacttatta
     4441 caagcatgag ggacaaagcc ccagggcagc tggagtgtga gacagccatt gcggctctga
     4501 acagctgctt gcgggaccta gatcaggctt cgcttgccgc tgtcagccag cagcttgctc
     4561 cccgtgaagg aatctctcaa gaggctttgc acacccagat gctcactgca gtgcaagaaa
     4621 tttctcatct cattgagccg ctggccagcg ctgctcgggc tgaagcctcc cagctgggac
     4681 acaaggtgtc ccaaatggcc cagtactttg aaccactcac cctggctgca gtgggtgctg
     4741 cgtctaagac cctgagccac ccgcagcaga tggcacttct ggaccagact aaaacgttgg
     4801 cagagtctgc cttgcagttg ctatatactg ccaaggaggc tggtggtaac cccaagcaag
     4861 cagcacacac ccaggaagcc ctggaggagg ctgtgcagat gatgacagag gccgtagaag
     4921 acctgacgac gaccctcaac gaagcagcca gtgctgcagg agtcgttggc ggcatggtgg
     4981 actctatcac tcaggccatc aaccagctag atgaaggacc tatgggtgac ccagaaggct
     5041 cattcgtaga ttaccagaca accatggtga ggacagccaa ggccattgct gtcactgttc
     5101 aggagatggt aaccaagtca aacaccagcc ctgaagagct aggccctctt gccaaccagc
     5161 tgaccagtga ctatggccga ctggcctcac aagccaagcc tgcagctgtg gctgctgaaa
     5221 atgaagagat aggcgctcac atcaaacacc gagtacagga gctgggccat ggctgctctg
     5281 ctctggtcac caaggcaggt gccttgcagt gtagccccag tgatgtctac accaagaagg
     5341 agctcataga gtgtgcccgc agagtgtcag agaaggtctc ccatgttctg gctgcactcc
     5401 aggctgggaa tcgtggtacc caggcctgca ttacagcagc cagtgctgtg tctggtatca
     5461 ttgctgacct cgacaccacc atcatgttcg ctactgctgg cacacttaac cgtgagggtg
     5521 ctgaaacttt tgctgaccac cgggagggta tcttaaagac agcaaaggtt cttgtggagg
     5581 acaccaaggt cctagtgcag aatgcagctg ggagccagga gaagttggca caagccgccc
     5641 agtcctccgt ggccaccatt acccgcctcg ctgatgtggt caagctcggt gcagccagcc
     5701 taggagccga agaccctgaa actcaggtgg tgctgatcaa tgcagtaaag gacgtagcca
     5761 aggccctggg tgacctcatc agcgctacga aggctgcagc gggcaaagtt ggggatgacc
     5821 ctgcagtgtg gcagctcaag aactctgcca aggtgatggt gaccaatgtg acatcattgc
     5881 tcaagacagt gaaggctgtg gaagatgagg ccaccaaagg cacacgggcc ctagagaaga
     5941 cctctacccc tgaagacttc atccgaatga ccaagggtat tactatggca acagccaaag
     6001 ccgttgctgc tggcaattcc tgtcgacagg aagatgtcat tgccacagcc aatctgagcc
     6061 gacgggctat tgcggacatg cttcgggctt gcaaggaagc agctttccac ccagaagtgg
     6121 cgcctgatgt acggctccga gccctgcact atggccggga gtgtgccaat ggttacctgg
     6181 aactgctgga ccacgtgctg ctgacccttc agaagccaaa cccagacctg aagcagcagc
     6241 taactggaca ctcaaagcgg gttgctggct ctgtgactga gctcatccag gctgctgaag
     6301 ccatgaaagg aacagagtgg gtggacccag aggaccctac tgtcattgct gagaatgagc
     6361 tcttgggagc cgcagccgcc atcgaggctg cagccaagaa gctggagcag ctgaagccca
     6421 gggccaaacc caaggaggcg gatgagtcct tgaactttga ggaacaaatc ctagaagctg
     6481 ccaagtccat cgctgcagcc accagtgcac tggtaaaggc tgcgtcagca gcccagaggg
     6541 agctggtggc tcaaggaaag gtgggcgcca ttccagccaa tgcactggat gatgggcagt
     6601 ggtcgcaggg cctcatttcc gctgcccgta tggtggctgc ggccaccaac aatctgtgtg
     6661 aagcagctaa cgcagctgtc cagggccatg ctagccagga gaaactcatt tcctcagcca
     6721 agcaagtagc tgcctccaca gctcagctcc tggtagcttg caaggtcaaa gcagatcagg
     6781 actctgaggc aatgaaacgg ctccaggctg ctggcaatgc agtgaagagg gcctcagata
     6841 acctggtaaa ggcggcccag aaagctgcag ccttcgaaga ccaggagaat gagacggtgg
     6901 tggtgaagga gaaaatggtt gggggcattg cccagattat cgcagcacag gaagagatgc
     6961 ttcggaagga acgagagctg gaagaggctc ggaaaaagct cgcccagatc cggcagcagc
     7021 agtacaagtt cttgccttca gagcttcgag acgagcacta aaaaagccct gtgtatttaa
     7081 tgcagaccca gcccagagac tgtgcctgcc actaccaaag ccttctgggc tgctcaggac
     7141 ccaagctgcc caaccccagc actcccccaa a
//
