LOCUS       sequence-8746-ex        9380 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9380)
  AUTHORS   Li QJ, Chau J, Ebert PJ, Sylvester G, Min H, Liu G, Braich R, 
            Manoharan M, Soutschek J, Skare P, Klein LO, Davis MM, Chen CZ
  TITLE     miR-181a is an intrinsic modulator of T cell sensitivity and 
            selection.
  JOURNAL   Cell. 2007 Apr 6. 129(1):147-61.
  PUBMED    17382377
REFERENCE   2  (bases 1 to 9380)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell. 2007 
            Apr 6. 129(1):147-61."
COMMENT     Sequence Label: MDH1-miR-181a1-Dusp6
FEATURES             Location/Qualifiers
     source          1..9380
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    580..921
                     /label=MESV Psi
                     /note="packaging signal of murine embryonic stem cell 
                     virus"
     CDS             988..1404
                     /label=gag (truncated)
                     /note="truncated Moloney murine leukemia virus (MMLV) gag 
                     gene lacking the start codon"
     primer_bind     1334..1356
                     /label=pLXSN 5'
                     /note="Murine stem cell virus, forward primer. Also called 
                     MSCV"
     primer_bind     1372..1388
                     /label=pBABE 5'
                     /note="Psi packaging signal, forward primer for pBABE 
                     vectors"
     primer_bind     1716..1732
                     /label=pBMN 5'
                     /note="MMLV sequence, for inserts in pBMN retroviral 
                     vector"
     promoter        1796..2296
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     2229..2248
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     CDS             join(3464..3466,3467..3469,3470..4183)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(3464..3466,3467..3469,3470..4180)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(3509..3530)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(3770..3789)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     4117..4138
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     misc_feature    4288..4749
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(4344..4361)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     4571..4590
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     promoter        complement(5585..5675)
                     /label=H1 promoter
                     /note="human H1 RNA promoter"
     primer_bind     complement(5645..5664)
                     /label=H1
                     /note="Human H1 promoter, forward primer"
     primer_bind     complement(6339..6355)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6339..6355)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(6352..6374)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    6363..6379
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(6387..6393,6394..6411,6412..6417))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6432..6453
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(6570..6587)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(6741..7329)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6821..6840)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(7500..8291,8292..8360))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8123..8142
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8361..8465)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     8533..8551
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(8589..8611)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     8711..8730
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     8924..8946
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     8938..8955
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     8939..8955
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     9158..9177
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     LTR             join(9379..9380,1..516)
                     /label=5' LTR
                     /note="5' long terminal repeat from murine embryonic stem 
                     cell virus"
ORIGIN
        1 tgaaagaccc cacctgtagg tttggcaagc tagcttaagt aacgccattt tgcaaggcat
       61 ggaaaataca taactgagaa tagagaagtt cagatcaagg ttaggaacag agagacagca
      121 gaatatgggc caaacaggat atctgtggta agcagttcct gccccggctc agggccaaga
      181 acagatggtc cccagatgcg gtcccgccct cagcagtttc tagagaacca tcagatgttt
      241 ccagggtgcc ccaaggacct gaaaatgacc ctgtgcctta tttgaactaa ccaatcagtt
      301 cgcttctcgc ttctgttcgc gcgcttctgc tccccgagct caataaaaga gcccacaacc
      361 cctcactcgg cgcgccagtc ctccgataga ctgcgtcgcc cgggtacccg tattcccaat
      421 aaagcctctt gctgtttgca tccgaatcgt ggactcgctg atccttggga gggtctcctc
      481 agattgattg actgcccacc tcgggggtct ttcatttgga ggttccaccg agatttggag
      541 acccctgcct agggaccacc gacccccccg ccgggaggta agctggccag cggtcgtttc
      601 gtgtctgtct ctgtctttgt gcgtgtttgt gccggcatct aatgtttgcg cctgcgtctg
      661 tactagttag ctaactagct ctgtatctgg cggacccgtg gtggaactga cgagttctga
      721 acacccggcc gcaaccctgg gagacgtccc agggactttg ggggccgttt ttgtggcccg
      781 acctgaggaa gggagtcgat gtggaatccg accccgtcag gatatgtggt tctggtagga
      841 gacgagaacc taaaacagtt cccgcctccg tctgaatttt tgctttcggt ttggaaccga
      901 agccgcgcgt cttgtctgct gcagcgctgc agcatcgttc tgtgttgtct ctgtctgact
      961 gtgtttctgt atttgtctga aaattagggc cagactgtta ccactccctt aagtttgacc
     1021 ttaggtcact ggaaagatgt cgagcggatc gctcacaacc agtcggtaga tgtcaagaag
     1081 agacgttggg ttaccttctg ctctgcagaa tggccaacct ttaacgtcgg atggccgcga
     1141 gacggcacct ttaaccgaga cctcatcacc caggttaaga tcaaggtctt ttcacctggc
     1201 ccgcatggac acccagacca ggtcccctac atcgtgacct gggaagcctt ggcttttgac
     1261 ccccctccct gggtcaagcc ctttgtacac cctaagcctc cgcctcctct tcctccatcc
     1321 gccccgtctc tcccccttga acctcctcgt tcgaccccgc ctcgatcctc cctttatcca
     1381 gccctcactc cttctctagg cgccggaatt agatcttata tggggcaccc ccgccccttg
     1441 taaacttccc tgaccctgac atgacaagag ttactaacag cccctctctc caagctcact
     1501 tacaggctct ctacttagtc cagcacgaag tctggagacc tctggcggca gcctaccaag
     1561 aacaactgga ccgaccggtg gtacctcacc cttaccgagt cggcgacaca gtgtgggtcc
     1621 gccgacacca gactaagaac ctagaacctc gctggaaagg accttacaca gtcctgctga
     1681 ccacccccac cgccctcaaa gtagacggca tcgcagcttg gatacacgcc gcccacgtga
     1741 aggctgccga ccccgggggt ggaccatcct ctagactgcc ggatcaattc ctaccgggta
     1801 ggggaggcgc ttttcccaag gcagtctgga gcatgcgctt tagcggcccc gctgggcact
     1861 tggcgctaca caagtggcct ctggcctcgc acacattcca catccaccgg taggcgccaa
     1921 ccggctccgt tctttggtgg ccccttcgcg ccaccttcta ctcctcccct agtcaggaag
     1981 ttcccccccg ccccgcagct cgcgtcgtgc aggacgtgac aaatggaagt agcacgtctc
     2041 actagtctcg tgcagatgga cagcaccgct gagcaatgga agcgggtagg cctttggggc
     2101 agcggccaat agcagctttg gctccttcgc tttctgggct cagaggctgg gaaggggtgg
     2161 gtccgggggc gggctcaggg gcgggctcag gggcggggcg ggcgcccgaa ggtcctccgg
     2221 aggcccggca ttctgcacgc ttcaaaagcg cacgtctgcc gcgctgttct cctcttcctc
     2281 atctccgggc ctttcgacct gcaccatggg catgatagat acgctcagac ccgtgccctt
     2341 cgcgtcggaa atggcgatct gcaagacggt gtcgtggctc aacgagcagc tggagctggg
     2401 caacgaacgg cttctgctga tggactgccg accacaggag ctgtacgagt cgtcacacat
     2461 cgaatctgcc attaatgtgg ccatccccgg catcatgctg cggcgtctgc agaagggcaa
     2521 cctgcccgtg cgtgcgctct tcacgcgctg cgaggaccgg gaccgcttta ccaggcgctg
     2581 cggcaccgac accgtggtgc tgtacgacga gaatagcagc gactggaatg agaacactgg
     2641 tggagagtcg gtcctcgggc tgctgctcaa gaaactcaaa gacgagggct gccgggcgtt
     2701 ctacctggaa ggtggcttca gtaagttcca ggccgagttc gccctgcact gcgagaccaa
     2761 tctagacggc tcgtgcagca gcagttcccc gcctttgcca gtgctggggc tcgggggcct
     2821 gcggatcagc tcggactctt cctcggacat tgagtctgac cttgaccgag accccaatag
     2881 tgcaacggac tctgatggca gcccgctgtc caacagccag ccttccttcc cggtggagat
     2941 tttgcccttc ctttacctgg gctgtgccaa ggactcgacc aacttggacg tgttggaaga
     3001 gtttggcatc aagtacatct tgaatgtcac ccccaatttg cccaatctgt ttgagaatgc
     3061 gggcgagttc aaatacaagc aaattcctat ctcggatcac tggagccaaa acctgtccca
     3121 gtttttccct gaggccattt ctttcataga tgaagcccga ggcaaaaact gtggtgtcct
     3181 ggtgcattgc ttggcaggta tcagccgctc tgtcaccgtg acagtggcgt acctcatgca
     3241 gaagctcaac ctgtccatga acgatgctta cgacattgtt aagatgaaga agtccaacat
     3301 ctcccccaac ttcaacttca tgggccagct gcttgacttc gaaaggaccc tgggactgag
     3361 cagcccttgt gacaaccgtg tccccactcc gcagctgtac ttcaccacgc cctccaacca
     3421 gaacgtctac caggtggact ccctgcagtc tacgcatgca ttcatggtga gcaagggcga
     3481 ggagctgttc accggggtgg tgcccatcct ggtcgagctg gacggcgacg taaacggcca
     3541 caagttcagc gtgtccggcg agggcgaggg cgatgccacc tacggcaagc tgaccctgaa
     3601 gttcatctgc accaccggca agctgcccgt gccctggccc accctcgtga ccaccctgac
     3661 ctacggcgtg cagtgcttca gccgctaccc cgaccacatg aagcagcacg acttcttcaa
     3721 gtccgccatg cccgaaggct acgtccagga gcgcaccatc ttcttcaagg acgacggcaa
     3781 ctacaagacc cgcgccgagg tgaagttcga gggcgacacc ctggtgaacc gcatcgagct
     3841 gaagggcatc gacttcaagg aggacggcaa catcctgggg cacaagctgg agtacaacta
     3901 caacagccac aacgtctata tcatggccga caagcagaag aacggcatca aggtgaactt
     3961 caagatccgc cacaacatcg aggacggcag cgtgcagctc gccgaccact accagcagaa
     4021 cacccccatc ggcgacggcc ccgtgctgct gcccgacaac cactacctga gcacccagtc
     4081 cgccctgagc aaagacccca acgagaagcg cgatcacatg gtcctgctgg agttcgtgac
     4141 cgccgccggg atcactctcg gcatggacga gctgtacaag taatgaggat ccccgggtac
     4201 cgagctcgaa ttcgagagta aaacaattga agatctagtc gaccaattcc ggttattttc
     4261 caccatattg ccgtcttttg gcaatgtgag ggcccggaaa cctggccctg tcttcttgac
     4321 gagcattcct aggggtcttt cccctctcgc caaaggaatg caaggtctgt tgaatgtcgt
     4381 gaaggaagca gttcctctgg aagcttcttg aagacaaaca acgtctgtag cgaccctttg
     4441 caggcagcgg aaccccccac ctggcgacag gtgcctctgc ggccaaaagc cacgtgtata
     4501 agatacacct gcaaaggcgg cacaacccca gtgccacgtt gtgagttgga tagttgtgga
     4561 aagagtcaaa tggctctcct caagcgtatt caacaagggg ctgaaggatg cccagaaggt
     4621 accccattgt atgggatctg atctggggcc tcggtgcaca tgctttacat gtgtttagtc
     4681 gaggttaaaa aacgtctagg ccccccgaac cacggggacg tggttttcct ttgaaaaaca
     4741 cgatgataat accatgaaaa catttaacat ttctcaacag gatctagaat tagtagaagt
     4801 agcgacagag aagattacaa tgctttatga ggataataaa catcatgtgg gagcggcaat
     4861 tcgtacgaaa acaggagaaa tcatttcggc agtacatatt gaagcgtata taggacgagt
     4921 aactgtttgt gcagaagcca ttgcgattgg tagtgcagtt tcgaatggac aaaaggattt
     4981 tgacacgatt gtagctgtta gacaccctta ttctgacgaa gtagatagaa gtattcgagt
     5041 ggtaagtcct tgtggtatgt gtagggagtt gatttcagac tatgcaccag attgttttgt
     5101 gttaatagaa atgaatggca agttagtcaa aactacgatt gaagaactca ttccactcaa
     5161 atatacccga aattaatgag ttaacttaat taagaattgc ggccgcgtcg acctgcagcc
     5221 aagcttatcg ataaaataaa agattttatt tagtctccag aaaaaggggg gaatgaaaga
     5281 ccccacctgt aggtttggca agaattctag gaattcttaa ttaaaaaaga atcctgtcgg
     5341 gtcctgcttt gcggttctgt cttcagcaaa cgcagagggc catggagtag atgatggtta
     5401 gctatagggt acaatcaacg gtcgatggtt tttatttgaa ttccaaactc accgacagcg
     5461 ttgaatgttc actgaagcaa cctgtgaggc agagatgtgc tgatttaaac agtatctgtg
     5521 ggagtctgga gcaggaggga ggcagaggaa agcccaatcc tttaaccaaa cctgtcacac
     5581 tcgagtggtc tcatacagaa cttataagat tcccaaatcc aaagacattt cacgtttatg
     5641 gtgatttccc agaacacata gcgacatgca aatattggga tccgctagct taagtaacgc
     5701 cattttgcaa ggcatggaaa atacataact gagaatagag aagttcagat caaggttagg
     5761 aacagagaga cagcagaata tgggccaaac aggatatctg tggtaagcag ttcctgcccc
     5821 ggctcagggc caagaacaga tggtccccag atgcggtccc gccctcagca gtttctagag
     5881 aaccatcaga tgtttccagg gtgccccaag gacctgaaaa tgaccctgtg ccttatttga
     5941 actaaccaat cagttcgctt ctcgcttctg ttcgcgcgct tctgctcccc gagctcaata
     6001 aaagagccca caacccctca ctcggcgcgc cagtcctccg atagactgcg tcgcccgggt
     6061 acccgtgtat ccaataaacc ctcttgcagt tgcatccgac ttgtggtctc gctgttcctt
     6121 gggagggtct cctctgagtg attgactacc cgtcagcggg ggtctttcat gggtaacagt
     6181 ttcttgaagt tggagaacaa cattctgagg gtaggagtcg aatattaagt aatcctgact
     6241 caattagcca ctgttttgaa tccacatact ccaatactcc tgaaatagtt cattatggac
     6301 agcgcagaag agctggggag aattaattcg taatcatggt catagctgtt tcctgtgtga
     6361 aattgttatc cgctcacaat tccacacaac atacgagccg gaagcataaa gtgtaaagcc
     6421 tggggtgcct aatgagtgag ctaactcaca ttaattgcgt tgcgctcact gcccgctttc
     6481 cagtcgggaa acctgtcgtg ccagctgcat taatgaatcg gccaacgcgc ggggagaggc
     6541 ggtttgcgta ttgggcgctc ttccgcttcc tcgctcactg actcgctgcg ctcggtcgtt
     6601 cggctgcggc gagcggtatc agctcactca aaggcggtaa tacggttatc cacagaatca
     6661 ggggataacg caggaaagaa catgtgagca aaaggccagc aaaaggccag gaaccgtaaa
     6721 aaggccgcgt tgctggcgtt tttccatagg ctccgccccc ctgacgagca tcacaaaaat
     6781 cgacgctcaa gtcagaggtg gcgaaacccg acaggactat aaagatacca ggcgtttccc
     6841 cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc
     6901 gcctttctcc cttcgggaag cgtggcgctt tctcatagct cacgctgtag gtatctcagt
     6961 tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt tcagcccgac
     7021 cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc cggtaagaca cgacttatcg
     7081 ccactggcag cagccactgg taacaggatt agcagagcga ggtatgtagg cggtgctaca
     7141 gagttcttga agtggtggcc taactacggc tacactagaa ggacagtatt tggtatctgc
     7201 gctctgctga agccagttac cttcggaaaa agagttggta gctcttgatc cggcaaacaa
     7261 accaccgctg gtagcggtgg tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa
     7321 ggatctcaag aagatccttt gatcttttct acggggtctg acgctcagtg gaacgaaaac
     7381 tcacgttaag ggattttggt catgagatta tcaaaaagga tcttcaccta gatcctttta
     7441 aattaaaaat gaagttttaa atcaatctaa agtatatatg agtaaacttg gtctgacagt
     7501 taccaatgct taatcagtga ggcacctatc tcagcgatct gtctatttcg ttcatccata
     7561 gttgcctgac tccccgtcgt gtagataact acgatacggg agggcttacc atctggcccc
     7621 agtgctgcaa tgataccgcg agacccacgc tcaccggctc cagatttatc agcaataaac
     7681 cagccagccg gaagggccga gcgcagaagt ggtcctgcaa ctttatccgc ctccatccag
     7741 tctattaatt gttgccggga agctagagta agtagttcgc cagttaatag tttgcgcaac
     7801 gttgttgcca ttgctacagg catcgtggtg tcacgctcgt cgtttggtat ggcttcattc
     7861 agctccggtt cccaacgatc aaggcgagtt acatgatccc ccatgttgtg caaaaaagcg
     7921 gttagctcct tcggtcctcc gatcgttgtc agaagtaagt tggccgcagt gttatcactc
     7981 atggttatgg cagcactgca taattctctt actgtcatgc catccgtaag atgcttttct
     8041 gtgactggtg agtactcaac caagtcattc tgagaatagt gtatgcggcg accgagttgc
     8101 tcttgcccgg cgtcaatacg ggataatacc gcgccacata gcagaacttt aaaagtgctc
     8161 atcattggaa aacgttcttc ggggcgaaaa ctctcaagga tcttaccgct gttgagatcc
     8221 agttcgatgt aacccactcg tgcacccaac tgatcttcag catcttttac tttcaccagc
     8281 gtttctgggt gagcaaaaac aggaaggcaa aatgccgcaa aaaagggaat aagggcgaca
     8341 cggaaatgtt gaatactcat actcttcctt tttcaatatt attgaagcat ttatcagggt
     8401 tattgtctca tgagcggata catatttgaa tgtatttaga aaaataaaca aataggggtt
     8461 ccgcgcacat ttccccgaaa agtgccacct gacgtctaag aaaccattat tatcatgaca
     8521 ttaacctata aaaataggcg tatcacgagg ccctttcgtc tcgcgcgttt cggtgatgac
     8581 ggtgaaaacc tctgacacat gcagctcccg gagacggtca cagcttgtct gtaagcggat
     8641 gccgggagca gacaagcccg tcagggcgcg tcagcgggtg ttggcgggtg tcggggctgg
     8701 cttaactatg cggcatcaga gcagattgta ctgagagtgc accatatgcg gtgtgaaata
     8761 ccgcacagat gcgtaaggag aaaataccgc atcaggcgcc attcgccatt caggctgcgc
     8821 aactgttggg aagggcgatc ggtgcgggcc tcttcgctat tacgccagct ggcgaaaggg
     8881 ggatgtgctg caaggcgatt aagttgggta acgccagggt tttcccagtc acgacgttgt
     8941 aaaacgacgg ccagtgccaa gctcgctctc ccttatgcga ctcctgcatt aggaagcagc
     9001 ccagtagtag gttgaggccg ttgagcaccg ccgccgcaag gaatggtgca tgcaaggaga
     9061 tggcgcccaa cagtcccccg gccacggggc ctgccaccat acccacgccg aaacaagcgc
     9121 tcatgagccc gaagtggcga gcccgatctt ccccatcggt gatgtcggcg atataggcgc
     9181 cagcaaccgc acctgtggcg ccggtgatgc cggccacgat gcgtccggcg tagaggatcg
     9241 attagtccaa tttgttaaag acaggatatc agtggtccag gctctagttt tgactcaaca
     9301 atatcaccag ctgaagccta tagagtacga gccatagata aaataaaaga ttttatttag
     9361 tctccagaaa aaggggggaa
//
