LOCUS       sequence-87460-e        1917 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Actin/Focal Adhesions, Excitation: 516, Emission: 529.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1917)
  TITLE     Davidson Yellow fluorescent proteins
REFERENCE   2  (bases 1 to 1917)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: mCitrine-VASP-5
FEATURES             Location/Qualifiers
     source          1..1917
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      28..37
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(34..36,37..39,40..750)
                     /codon_start=1
                     /product="enhanced YFP with monomerizing A206K mutation 
                     (Zacharias et al., 2002)"
                     /label=mEYFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTFGYGLMCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSYQSKLSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(79..100)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(340..359)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     687..708
                     /label=EGFP-C
                     /note="EGFP, forward primer"
ORIGIN
        1 acgtcagatc cgctagcgct accggtcgcc accatggtga gcaagggcga ggagctgttc
       61 accggggtgg tgcccatcct ggtcgagctg gacggcgacg taaacggcca caagttcagc
      121 gtgtccggcg agggcgaggg cgatgccacc tacggcaagc tgaccctgaa gttcatctgc
      181 accaccggca agctgcccgt gccctggccc accctcgtga ccaccttcgg ctacggcctg
      241 atgtgcttcg cccgctaccc cgaccacatg aagcagcacg acttcttcaa gtccgccatg
      301 cccgaaggct acgtccagga gcgcaccatc ttcttcaagg acgacggcaa ctacaagacc
      361 cgcgccgagg tgaagttcga gggcgacacc ctggtgaacc gcatcgagct gaagggcatc
      421 gacttcaagg aggacggcaa catcctgggg cacaagctgg agtacaacta caacagccac
      481 aacgtctata tcatggccga caagcagaag aacggcatca aggtgaactt caagatccgc
      541 cacaacatcg aggacggcag cgtgcagctc gccgaccact accagcagaa cacccccatc
      601 ggcgacggcc ccgtgctgct gcccgacaac cactacctga gctaccagtc caagctgagc
      661 aaagacccca acgagaagcg cgatcacatg gtcctgctgg agttcgtgac cgccgccggg
      721 atcactctcg gcatggacga gctgtacaag tccggactca gatctatgag cgagacggtc
      781 atctgttcca gccgggctac tgtgatgctt tatgatgaca gcaacaagcg atggctccct
      841 gctggcactg gtccccaggc cttcagccgc gtccagatat accacaaccc cactgctaac
      901 tccttccgag ttgttggccg caagatgcag ccggatcagc aggtggttat caactgtgcc
      961 atcattcggg gtgtcaagta caatcaggcc actcccatct tccatcagtg gcgagatgcc
     1021 cgccaggtct ggggcctcaa cttcggcagc aaggaggacg ccatacagtt tgcaacaggc
     1081 atggccaacg ccctagaggc cttggaagga ggtgggcctc ccccagcccc agcaccccct
     1141 gcctggtctg cccagaatgg tccctcccca gaggagctgg aacaacagaa aaggcagccg
     1201 gagcatatgg agcgccgggt ctccaatgca ggaggcccac ctgctccccc agctgggggc
     1261 cctcctccac ctccaggacc tccccctcct ccaggtcccc ccccaccccc aggtctgccc
     1321 tcctcagggg tatctggggc aggtcatgga gcaggggcag ccccaccccc tgcaccccca
     1381 ctccctacaa cacagggccc caatagtggg ggttccgggg ccccaggcct ggctgctgcc
     1441 attgctggag ccaaactcag gaaagtgagc aagcaggagg aggcctctgg ggggcccctg
     1501 gcccccaaag ctgagaacag tcgaagcact ggtggggggc ttatggaaga gatgaacgcc
     1561 atgctggccc ggagaagaaa agccacacag gttggggaga agccccccaa agacgagtca
     1621 gccagtgagg agtcagaggc ccgactccct gcccagagtg aacctgtgag aagaccctgg
     1681 gagaagaaca gcacaacctt gccaaggatg aagtcgtcct cttctgtgac tacctccgag
     1741 gcccacccct cgacgccctg ctccagtgat gactccgact tggagagggt gaagcaggag
     1801 cttctggaag aggtgcggaa ggagctacag aaaatgaaag aggaaatcat cgaagtcttt
     1861 gtccaggagc tgaggaagcg gggttctcct tgagcggccg cgactctaga tcataat
//
