LOCUS       sequence-87461-e        4044 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Focal Adhesions, Excitation: 516, Emission: 529.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 4044)
  TITLE     Davidson Yellow fluorescent proteins
REFERENCE   2  (bases 1 to 4044)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: mCitrine-Vinculin-23
FEATURES             Location/Qualifiers
     source          1..4044
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      13..22
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(19..21,22..24,25..735)
                     /codon_start=1
                     /product="enhanced YFP with monomerizing A206K mutation 
                     (Zacharias et al., 2002)"
                     /label=mEYFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTFGYGLMCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSYQSKLSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(64..85)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(325..344)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     672..693
                     /label=EGFP-C
                     /note="EGFP, forward primer"
ORIGIN
        1 gcgctaccgg tcgccaccat ggtgagcaag ggcgaggagc tgttcaccgg ggtggtgccc
       61 atcctggtcg agctggacgg cgacgtaaac ggccacaagt tcagcgtgtc cggcgagggc
      121 gagggcgatg ccacctacgg caagctgacc ctgaagttca tctgcaccac cggcaagctg
      181 cccgtgccct ggcccaccct cgtgaccacc ttcggctacg gcctgatgtg cttcgcccgc
      241 taccccgacc acatgaagca gcacgacttc ttcaagtccg ccatgcccga aggctacgtc
      301 caggagcgca ccatcttctt caaggacgac ggcaactaca agacccgcgc cgaggtgaag
      361 ttcgagggcg acaccctggt gaaccgcatc gagctgaagg gcatcgactt caaggaggac
      421 ggcaacatcc tggggcacaa gctggagtac aactacaaca gccacaacgt ctatatcatg
      481 gccgacaagc agaagaacgg catcaaggtg aacttcaaga tccgccacaa catcgaggac
      541 ggcagcgtgc agctcgccga ccactaccag cagaacaccc ccatcggcga cggccccgtg
      601 ctgctgcccg acaaccacta cctgagctac cagtccaagc tgagcaaaga ccccaacgag
      661 aagcgcgatc acatggtcct gctggagttc gtgaccgccg ccgggatcac tctcggcatg
      721 gacgagctgt acaagtccgg actcagatct cgagctcaag cttcgaattc gggaaactcg
      781 agaacaaagc ttgaaaacgg ccgcatgcca gtgtttcata cgcgcacgat cgagagcatc
      841 ctggagccgg tggcacagca gatctcccac ctggtgataa tgcacgagga gggcgaggtg
      901 gacggcaaag ccattcctga cctcaccgcg cccgtggccg ccgtgcaggc ggccgtcagc
      961 aacctcgtcc gggttggaaa agagactgtt caaaccactg aggatcagat tttgaagaga
     1021 gatatgccac cagcatttat taaggttgag aatgcttgca ccaagcttgt ccaggcagct
     1081 cagatgcttc agtcagaccc ttactcagtg cctgctcgag attatctaat tgatgggtca
     1141 aggggcatcc tctctggaac atcagacctg ctccttacct tcgatgaggc tgaggtccgt
     1201 aaaattatta gagtttgcaa aggaattttg gaatatctta cagtggcaga ggtggtggag
     1261 actatggaag atttggtcac ttacacaaag aatcttgggc caggaatgac taagatggcc
     1321 aagatgattg acgagagaca gcaggagctc actcaccagg agcaccgagt gatgttggtg
     1381 aactcgatga acaccgtgaa agagttgctg ccagttctca tttcagctat gaagattttt
     1441 gtaacaacta aaaactcaaa aaaccaaggc atagaggaag ctttaaaaaa tcgcaatttt
     1501 actgtagaaa aaatgagtgc tgaaattaat gagataattc gtgtgttaca actcacctct
     1561 tgggatgaag atgcctgggc cagcaaggac actgaagcca tgaagagagc attggcctcc
     1621 atagactcca aactgaacca ggccaaaggt tggctccgtg accctagtgc ctccccaggg
     1681 gatgctggtg agcaggccat cagacagatc ttagatgaag ctggaaaagt tggtgaactc
     1741 tgtgcaggca aagaacgcag ggagattctg ggaacttgca aaatgctagg gcagatgact
     1801 gatcaagtgg ctgacctccg tgccagagga caaggatcct caccggtggc catgcagaaa
     1861 gctcagcagg tatctcaggg tctggatgtg ctcacagcaa aagtggaaaa tgcagctcgc
     1921 aagctggaag ccatgaccaa ctcaaagcag agcattgcaa agaagatcga tgctgctcag
     1981 aactggcttg cagatccaaa tggtggaccg gaaggagaag agcagattcg aggtgctttg
     2041 gctgaagctc ggaaaatagc agaattatgt gatgatccta aagaaagaga tgacattcta
     2101 cgttcccttg gggaaatatc tgctctgact tctaaattag cagatctacg aagacagggg
     2161 aaaggagatt ctccagaggc tcgagccttg gccaaacagg tggccacggc cctgcagaac
     2221 ctgcagacca aaaccaaccg ggctgtggcc aacagcagac cggccaaagc agctgtacac
     2281 cttgagggca agattgagca agcacagcgg tggattgata atcccacagt ggatgaccgt
     2341 ggagtcggtc aggctgccat ccgggggctt gtggccgaag ggcatcgtct ggctaatgtt
     2401 atgatggggc cttatcggca agatcttctc gccaagtgtg accgagtgga ccagctgaca
     2461 gcccagctgg ctgacctggc tgccagaggg gaaggggaga gtcctcaggc acgagcactt
     2521 gcatctcagc tccaagactc cttaaaggat ctaaaagctc ggatgcagga ggccatgact
     2581 caggaagtgt cagatgtttt cagcgatacc acaactccca tcaagctgtt ggcagtggca
     2641 gccacggcgc ctcctgatgc gcctaacagg gaagaggtat ttgatgagag ggcagctaac
     2701 tttgaaaacc attcaggaaa gcttggtgct acggccgaga aggcggctgc ggttggtact
     2761 gctaataaat caacagtgga aggcattcag gcctcagtga agacggcccg agaactcaca
     2821 ccccaggtgg tctcggctgc tcgtatctta cttaggaacc ctggaaatca agctgcttat
     2881 gaacattttg agaccatgaa gaaccagtgg atcgataatg ttgaaaaaat gacagggctg
     2941 gtggacgaag ccattgatac caaatctctg ttggatgctt cagaagaagc aattaaaaaa
     3001 gacctggaca agtgcaaggt agctatggcc aacattcagc ctcagatgct ggttgctggg
     3061 gcaaccagta ttgctcgtcg ggccaaccgg atcctgctgg tggctaagag ggaggtggag
     3121 aattccgagg atcccaagtt ccgtgaggct gtgaaagctg cctctgatga attgagcaaa
     3181 accatctccc cgatggtgat ggatgcaaaa gctgtggctg gaaacatttc cgaccctgga
     3241 ctgcaaaaga gcttcctgga ctcaggatat cggatcctgg gagctgtggc caaggtcaga
     3301 gaagccttcc aacctcagga gcctgacttc ccgccgcctc caccagacct tgaacaactc
     3361 cgactaacag atgagcttgc tcctcccaaa ccacctctgc ctgaaggtga ggtccctcca
     3421 cctaggcctc caccaccaga ggaaaaggat gaagagttcc ctgagcagaa ggccggggag
     3481 gtgattaacc agccaatgat gatggctgcc agacagctcc atgatgaagc tcgcaaatgg
     3541 tccagcaagg gcaatgacat cattgcagca gccaagcgca tggctctgct gatggctgag
     3601 atgtctcggc tggtaagagg gggcagtggt accaagcggg cactcattca gtgtgccaag
     3661 gacatcgcca aggcctcaga tgaggtgact cggttggcca aggaggttgc caagcagtgc
     3721 acagataaac ggattagaac caacctctta caggtatgtg agcgaatccc aaccataagc
     3781 acccagctca aaatcctgtc cacagtgaag gccaccatgc tgggccggac caacatcagt
     3841 gatgaggagt ctgagcaggc cacagagatg ctggttcaca atgcccagaa cctcatgcag
     3901 tctgtgaagg agactgtgcg ggaagctgaa gctgcttcaa tcaaaattcg aacagatgct
     3961 ggatttacac tgcgctgggt tagaaagact ccctggtacc agtaggcggc cgcgactcta
     4021 gatcataatc agccatacca catt
//
