LOCUS       sequence-87463-e        1835 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Lysosomes, Excitation: 514, Emission: 527.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1835)
  TITLE     Davidson Yellow fluorescent proteins
REFERENCE   2  (bases 1 to 1835)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: mTopaz-CathespinB-6
FEATURES             Location/Qualifiers
     source          1..1835
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      1071..1080
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(1077..1079,1080..1082,1083..1796)
                     /codon_start=1
                     /product="enhanced yellow variant of GFP"
                     /label=Topaz YFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTFGYGVQCFARYPDHMRQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSYQSKLSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(1122..1143)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(1383..1402)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     1730..1751
                     /label=EGFP-C
                     /note="EGFP, forward primer"
ORIGIN
        1 acgtcagatc cgctagcgct accggactca gatctcgagc tcaagcttcg aattccagga
       61 tgtggtggtc cttgatccct ctctcttgcc tgctggcact gaccagtgcc catgacaagc
      121 cttcctttca cccactgtcg gacgacatga ttaactatat caacaaacag aatacaacat
      181 ggcaggctgg acgcaacttc tacaatgttg acataagcta tctgaagaag ctgtgtggaa
      241 ctgtcctggg tggacccaag ctgccggaaa gggttgggtt cagcgaggac ataaatctac
      301 ctgaatcctt tgatgcacgg gaacagtggt ccaattgccc gaccatcgca cagatcagag
      361 accaggggtc ctgtggctct tgttgggcat ttggggcagt ggaagccatg tctgaccgaa
      421 tctgcattca caccaatggc cgagtcaatg tggaggtgtc tgctgaggac ctgcttacct
      481 gctgtggtat ccagtgtggg gatggctgta atggtggcta tccctctgga gcatggaact
      541 tctggactag aaaaggcctg gtttctggtg gagtatacaa ttctcatata ggctgcttac
      601 cctacaccat ccctccctgt gaacaccatg tcaatggctc ccgtccccca tgcactggag
      661 aaggagatac tcccaagtgc aacaagatgt gtgaggctgg ctactccaca tcctacaagg
      721 aagataagca ctatgggtac acttcctaca gtgtgtctga cagcgagaag gagatcatgg
      781 cggaaatcta caaaaatggc ccagtggagg gtgcttttac tgtgttttct gacttcttga
      841 cttacaaatc aggcgtatac aagcatgaag ccggtgatgt gatgggaggc catgccatcc
      901 gcattctggg ctggggaata gagaatggag taccctactg gctggtagca aactcctgga
      961 acgttgactg gggtgataat ggtttcttta aaatcctcag aggagagaac cactgtggaa
     1021 ttgaatcaga aatcgtggct ggaatcccac gcactcagga tccaccggtc gccaccatgg
     1081 tgagcaaggg cgaggagctg ttcaccgggg tggtgcccat cctggtcgag ctggacggcg
     1141 acgtaaacgg ccacaagttc agcgtgtccg gcgagggcga gggcgatgcc acctacggca
     1201 agctgaccct gaagttcatc tgcaccaccg gcaagctgcc cgtgccctgg cccaccctcg
     1261 tgaccacctt cggctacggc gtgcagtgct tcgcccgcta ccccgaccac atgcgccagc
     1321 acgacttctt caagtccgcc atgcccgaag gctacgtcca ggagcgcacc atcttcttca
     1381 aggacgacgg caactacaag acccgcgccg aggtgaagtt cgagggcgac accctggtga
     1441 accgcatcga gctgaagggc atcgacttca aggaggacgg caacatcctg gggcacaagc
     1501 tggagtacaa ctacaacagc cacaacgtct atatcatggc cgacaagcag aagaacggca
     1561 tcaaggtgaa cttcaagatc cgccacaaca tcgaggacgg cagcgtgcag ctcgccgacc
     1621 actaccagca gaacaccccc atcggcgacg gccccgtgct gctgcccgac aaccactacc
     1681 tgagctacca gtccaagctg agcaaagacc ccaacgagaa gcgcgatcac atggtcctgc
     1741 tggagttcgt gaccgccgcc gggatcactc tcggcatgga cgagctgtac aagtaagcgg
     1801 ccgcgactct agatcataat cagccatacc acatt
//
