LOCUS       sequence-87467-e        1235 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Nucleus/Histones, Excitation: 508, Emission: 524.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1235)
  TITLE     Davidson Yellow fluorescent proteins
REFERENCE   2  (bases 1 to 1235)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: mTagYFP-H2B-6
FEATURES             Location/Qualifiers
     source          1..1235
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             80..457
                     /codon_start=1
                     /gene="HIST1H2BJ"
                     /product="human histone H2B"
                     /label=H2B
                     /translation="MPEPAKSAPAPKKGSKKAVTKAQKKGGKKRKRSRKESYSIYVYKV
                     LKQVHPDTGISSKAMGIMNSFVNDIFERIAGEASRLAHYNKRSTITSREIQTAVRLLLP
                     GELAKHAVSEGTKAITKYTSAK"
     regulatory      470..479
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             476..1195
                     /codon_start=1
                     /product="monomeric yellow fluorescent protein"
                     /label=TagYFP
                     /note="mammalian codon optimized"
                     /translation="MVSKGEELFAGIVPVLIELDGDVHGHKFSVRGEGEGDADYGKLEI
                     KFICTTGKLPVPWPTLVTTLTYGVQCFARYPKHMKMNDFFKSAMPEGYIQERTILFQDD
                     GKYKTRGEVKFEGDTLVNRIELKGKDFKEDGNILGHKLEYSFNSHNVYITPDKANNGLE
                     VNFKTRHNIEGGGVQLADHYQTNVPLGDGPVLIPINHYLSYQTDISKDRNEARDHMVLL
                     ESVSACSHTHGMDELYR"
ORIGIN
        1 aaccgtcaga tccgctagcg ctaccggact cagatctcga gctcaagctt cgaattctgc
       61 agtcgacggt accgccacca tgccagagcc agcgaagtct gctcccgccc cgaaaaaggg
      121 ctccaagaag gcggtgacta aggcgcagaa gaaaggcggc aagaagcgca agcgcagccg
      181 caaggagagc tattccatct atgtgtacaa ggttctgaag caggtccacc ctgacaccgg
      241 catttcgtcc aaggccatgg gcatcatgaa ttcgtttgtg aacgacattt tcgagcgcat
      301 cgcaggtgag gcttcccgcc tggcgcatta caacaagcgc tcgaccatca cctccaggga
      361 gatccagacg gccgtgcgcc tgctgctgcc tggggagttg gccaagcacg ccgtgtccga
      421 gggtactaag gccatcacca agtacaccag cgctaaggat ccaccggtcg ccaccatggt
      481 tagcaaaggc gaggagctgt tcgccggcat cgtgcccgtg ctgatcgagc tggacggcga
      541 cgtgcacggc cacaagttca gcgtgcgcgg cgagggcgag ggcgacgccg actacggcaa
      601 gctggagatc aagttcatct gcaccaccgg caagctgccc gtgccctggc ccaccctggt
      661 gaccaccctc acctacggcg tacagtgctt cgcccgctac cccaagcaca tgaagatgaa
      721 cgacttcttc aagagcgcca tgcccgaggg ctacatccag gagcgcacca tcctcttcca
      781 agacgacggc aagtacaaga cccgcggcga ggtgaagttc gagggcgaca ccctggtgaa
      841 ccgcatcgag ctgaagggca aggacttcaa ggaggacggc aacatcctgg gccacaagct
      901 ggagtacagc ttcaacagcc acaacgtcta catcaccccc gacaaggcca acaacggcct
      961 ggaggtgaac ttcaagaccc gccacaacat cgagggcggc ggcgtgcagc tggccgacca
     1021 ctaccagacc aacgtgcccc tgggcgacgg ccccgtgctg atccccatca accactacct
     1081 gagctaccag accgacatca gcaaggaccg caacgaggcc cgcgaccaca tggtgctcct
     1141 ggagtccgtc agcgcctgca gccacaccca cggcatggac gagctgtacc gctaaagcgg
     1201 ccgcgactct agatcataat cagccatacc acatt
//
