LOCUS       sequence-87473-e        1515 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Clathrin Vesicles, Excitation: 513, Emission: 527.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1515)
  AUTHORS   Rizzo MA, Davidson MW, Piston DW
  TITLE     Fluorescent protein tracking and detection: fluorescent protein 
            structure and color variants.
  JOURNAL   Cold Spring Harb Protoc. 2009 Dec;2009(12):pdb.top63. doi: 
            10.1101/pdb.top63.
  PUBMED    20150100
REFERENCE   2  (bases 1 to 1515)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cold Spring
            Harb Protoc."; date: "2009-12"; volume: "2009(12):pdb.top63. doi"; 
            pages: " 10.1101/pdb.top63"
COMMENT     Sequence Label: EYFP-Clathrin-19
FEATURES             Location/Qualifiers
     source          1..1515
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             join(35..37,38..40,41..751)
                     /codon_start=1
                     /product="enhanced YFP"
                     /label=EYFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTFGYGLQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSYQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(80..101)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(341..360)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     688..709
                     /label=EGFP-C
                     /note="EGFP, forward primer"
ORIGIN
        1 gacgtcagat ccgctagcgc taccggtcgc ccatatggtg agcaagggcg aggagctgtt
       61 caccggggtg gtgcccatcc tggtcgagct ggacggcgac gtaaacggcc acaagttcag
      121 cgtgtccggc gagggcgagg gcgatgccac ctacggcaag ctgaccctga agttcatctg
      181 caccaccggc aagctgcccg tgccctggcc caccctcgtg accaccttcg gctacggcct
      241 gcagtgcttc gcccgctacc ccgaccacat gaagcagcac gacttcttca agtccgccat
      301 gcccgaaggc tacgtccagg agcgcaccat cttcttcaag gacgacggca actacaagac
      361 ccgcgccgag gtgaagttcg agggcgacac cctggtgaac cgcatcgagc tgaagggcat
      421 cgacttcaag gaggacggca acatcctggg gcacaagctg gagtacaact acaacagcca
      481 caacgtctat atcatggccg acaagcagaa gaacggcatc aaggtgaact tcaagatccg
      541 ccacaacatc gaggacggca gcgtgcagct cgccgaccac taccagcaga acacccccat
      601 cggcgacggc cccgtgctgc tgcccgacaa ccactacctg agctaccagt ccgccctgag
      661 caaagacccc aacgagaagc gcgatcacat ggtcctgctg gagttcgtga ccgccgccgg
      721 gatcactctc ggcatggacg agctgtacaa gtactcagat ctcgagctca agcttcgaat
      781 tctgcagtcg agctcaagct tcgaattcat ggctgatgac tttggcttct tctcgtcgtc
      841 ggagagtggt gccccggagg cggcggagga ggacccggcg gccgccttcc tggcccagca
      901 ggagagcgag attgcaggca tagagaacga cgagggcttc ggggcacctg ccggcagcca
      961 tgcggccccc gcacagccgg gccccacgag tggggctggt tctgaggaca tggggaccac
     1021 agtcaatgga gatgtgtttc aggaggccaa cggtcctgct gatggctacg cagccattgc
     1081 ccaggctgac aggctgaccc aggagcctga gagcatccgc aagtggcgag aggagcagag
     1141 gaaacggctg caagagctgg atgctgcatc taaggtcacg gaacaggaat ggcgggagaa
     1201 ggccaagaag gacctggagg agtggaacca gcgccagagt gaacaagtag agaagaacaa
     1261 gatcaacaac cgggcatccg aggaggcttt cgtgaaggaa tccaaggagg agaccccagg
     1321 cacagagtgg gagaaggtgg cccagctatg tgacttcaac cccaagagca gcaagcagtg
     1381 caaagatgtg tcccgcctgc gctcggtgct catgtccctg aagcagacgc cactgtcccg
     1441 ctaggtcgac ggtaccgcgg gcccgggatc caccggatct agataactga tcataatcag
     1501 ccataccaca ttgag
//
