LOCUS       sequence-87475-e        1751 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1751)
  TITLE     Davidson Yellow fluorescent proteins
REFERENCE   2  (bases 1 to 1751)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: EYFP-Cx32-7
FEATURES             Location/Qualifiers
     source          1..1751
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      983..992
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(989..991,992..994,995..1708)
                     /codon_start=1
                     /product="enhanced YFP"
                     /label=EYFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTFGYGLQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSYQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(1034..1055)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(1295..1314)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     1642..1663
                     /label=EGFP-C
                     /note="EGFP, forward primer"
ORIGIN
        1 gacgtcagat ccgctagcgc taccggactc agatctcgag ctcaagcttc gaattccctc
       61 tgggaaaggg cagcagcagc caggtgtggc agtgacaggg aggtgtgaat gaggcaggat
      121 gaactggaca ggtttgtaca ccttgctcag tggcgtgaac cggcattcta ctgccattgg
      181 ccgagtatgg ctctcggtca tcttcatctt cagaatcatg gtgctggtgg tggctgcaga
      241 gagtgtgtgg ggtgatgaga aatcttcctt catctgcaac acactccagc ctggctgcaa
      301 cagcgtttgc tatgaccaat tcttccccat ctcccatgtg cggctgtggt ccctgcagct
      361 catcctagtt tccaccccag ctctcctcgt ggccatgcac gtggctcacc agcaacacat
      421 agagaagaaa atgctacggc ttgagggcca tggggacccc ctacacctgg aggaggtgaa
      481 gaggcacaag gtccacatct cagggacact gtggtggacc tatgtcatca gcgtggtgtt
      541 ccggctgttg tttgaggccg tcttcatgta tgtcttttat ctgctctacc ctggctatgc
      601 catggtgcgg ctggtcaagt gcgacgtcta cccctgcccc aacacagtgg actgcttcgt
      661 gtcccgcccc accgagaaaa ccgtcttcac cgtcttcatg ctagctgcct ctggcatctg
      721 catcatcctc aatgtggccg aggtggtgta cctcatcatc cgggcctgtg cccgccgagc
      781 ccagcgccgc tccaatccac cttcccgcaa gggctcgggc ttcggccacc gcctctcacc
      841 tgaatacaag cagaatgaga tcaacaagct gctgagtgag caggatggct ccctgaaaga
      901 catactgcgc cgcagccctg gcaccggggc tgggctggct gaaaagagcg accgctgctc
      961 ggcctgcgcg gatccaccgg tcgccaccat ggtgagcaag ggcgaggagc tgttcaccgg
     1021 ggtggtgccc atcctggtcg agctggacgg cgacgtaaac ggccacaagt tcagcgtgtc
     1081 cggcgagggc gagggcgatg ccacctacgg caagctgacc ctgaagttca tctgcaccac
     1141 cggcaagctg cccgtgccct ggcccaccct cgtgaccacc ttcggctacg gcctgcagtg
     1201 cttcgcccgc taccccgacc acatgaagca gcacgacttc ttcaagtccg ccatgcccga
     1261 aggctacgtc caggagcgca ccatcttctt caaggacgac ggcaactaca agacccgcgc
     1321 cgaggtgaag ttcgagggcg acaccctggt gaaccgcatc gagctgaagg gcatcgactt
     1381 caaggaggac ggcaacatcc tggggcacaa gctggagtac aactacaaca gccacaacgt
     1441 ctatatcatg gccgacaagc agaagaacgg catcaaggtg aacttcaaga tccgccacaa
     1501 catcgaggac ggcagcgtgc agctcgccga ccactaccag cagaacaccc ccatcggcga
     1561 cggccccgtg ctgctgcccg acaaccacta cctgagctac cagtccgccc tgagcaaaga
     1621 ccccaacgag aagcgcgatc acatggtcct gctggagttc gtgaccgccg ccgggatcac
     1681 tctcggcatg gacgagctgt acaagtaaag cggccgcgac tctagatcat aatcagccat
     1741 accacattga g
//
