LOCUS       sequence-87477-e        1680 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Endosomes, Excitation: 513, Emission: 527.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1680)
  TITLE     Davidson Yellow fluorescent proteins
REFERENCE   2  (bases 1 to 1680)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: EYFP-Endosomes-13
FEATURES             Location/Qualifiers
     source          1..1680
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        27..78
                     /label=CMVd2 promoter
                     /note="truncated human cytomegalovirus (CMV) immediate 
                     early promoter that yields moderately reduced expression 
                     relative to the full-length promoter"
     primer_bind     29..49
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     regulatory      117..126
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(123..125,126..128,129..839)
                     /codon_start=1
                     /product="enhanced YFP"
                     /label=EYFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTFGYGLQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSYQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(168..189)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(429..448)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     776..797
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     CDS             882..911
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
ORIGIN
        1 aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt acggtgggag
       61 gtctatataa gcagagctgg tttagtgaac cgtcagatcc gctagcgcta ccggtcgcca
      121 ccatggtgag caagggcgag gagctgttca ccggggtggt gcccatcctg gtcgagctgg
      181 acggcgacgt aaacggccac aagttcagcg tgtccggcga gggcgagggc gatgccacct
      241 acggcaagct gaccctgaag ttcatctgca ccaccggcaa gctgcccgtg ccctggccca
      301 ccctcgtgac caccttcggc tacggcctgc agtgcttcgc ccgctacccc gaccacatga
      361 agcagcacga cttcttcaag tccgccatgc ccgaaggcta cgtccaggag cgcaccatct
      421 tcttcaagga cgacggcaac tacaagaccc gcgccgaggt gaagttcgag ggcgacaccc
      481 tggtgaaccg catcgagctg aagggcatcg acttcaagga ggacggcaac atcctggggc
      541 acaagctgga gtacaactac aacagccaca acgtctatat catggccgac aagcagaaga
      601 acggcatcaa ggtgaacttc aagatccgcc acaacatcga ggacggcagc gtgcagctcg
      661 ccgaccacta ccagcagaac acccccatcg gcgacggccc cgtgctgctg cccgacaacc
      721 actacctgag ctaccagtcc gccctgagca aagaccccaa cgagaagcgc gatcacatgg
      781 tcctgctgga gttcgtgacc gccgccggga tcactctcgg catggacgag ctgtacaagt
      841 actcagatct cgagctcaag cttgatatcg aattccccat ggagcagaag ctgatctccg
      901 aggaggacct gaaccggaag aaactggtgg ttgttggtga tggagcctgt ggaaagacat
      961 gcttgctcat agtcttcagc aaggacgagt tcccagaggt gtatgtgccc acagtgtttg
     1021 agaactatgt ggcagatatc gaggtggatg gaaagcaggt agagttggct ttgtgggaca
     1081 cagctggcca ggaggactac gaccgcctgc ggccgctctc ctacccggac accgacgtca
     1141 ttctcatgtg cttctcggtg gacagcccgg actcgctgga gaacatcccc gagaagtggg
     1201 tccccgaggt gaagcacttc tgtcccaatg tgcccatcat cctggtggcc aacaaaaaag
     1261 acctgcgcag cgacgagcat gtccgcacag agctggcccg catgaagcag gaacccgttc
     1321 gcacggatga cggccgcgcc atggccgtgc gcatccaagc ctacgactac ctcgagtgct
     1381 ctgccaagac caaggaaggc gtgcgcgagg tcttcgagac ggccacgcgc gccgcgctgc
     1441 agaagcgcta cggctcccag aacggctgca tcaactgctg caaggtgcta tgagggccgc
     1501 gcccgtcgcg cctgccccgg cccgggatcc accggatcta gataactgat cataatcagc
     1561 cataccacat ttgtagaggt tttacttgct ttaaaaaacc tcccacacct ccccctgaac
     1621 ctgaaacata aaatgaatgc aattgttgtt gttaacttgt ttattgcagc ttataatggt
//
