LOCUS       sequence-87479-e        1167 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Golgi, Excitation: 513, Emission: 527.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1167)
  AUTHORS   Olenych SG, Claxton NS, Ottenberg GK, Davidson MW
  TITLE     The fluorescent protein color palette.
  JOURNAL   Curr Protoc Cell Biol. 2007 Sep;Chapter 21:Unit 21.5. doi: 
            10.1002/0471143030.cb2105s36.
  PUBMED    18228502
REFERENCE   2  (bases 1 to 1167)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Curr Protoc
            Cell Biol."; date: "2007-09"; volume: "Chapter 21:Unit 21.5. doi"; 
            pages: " 10.1002/0471143030.cb2105s36"
COMMENT     Sequence Label: EYFP-Golgi-7
FEATURES             Location/Qualifiers
     source          1..1167
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        4..55
                     /label=CMVd2 promoter
                     /note="truncated human cytomegalovirus (CMV) immediate 
                     early promoter that yields moderately reduced expression 
                     relative to the full-length promoter"
     primer_bind     6..26
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     regulatory      345..354
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(351..353,354..356,357..1070)
                     /codon_start=1
                     /product="enhanced YFP"
                     /label=EYFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTFGYGLQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSYQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(396..417)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(657..676)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     1004..1025
                     /label=EGFP-C
                     /note="EGFP, forward primer"
ORIGIN
        1 attgacgcaa atgggcggta ggcgtgtacg gtgggaggtc tatataagca gagctggttt
       61 agtgaaccgt cagatccgct agcatgaggc ttcgggagcc gctcctgagc ggcagcgccg
      121 cgatgccagg cgcgtcccta cagcgggcct gccgcctgct cgtggccgtc tgcgctctgc
      181 accttggcgt caccctcgtt tactacctgg ctggccgcga cctgagccgc ctgccccaac
      241 tggtcggagt ctccacaccg ctgcagggcg gctcgaacag tgccgccgcc atcgggcagt
      301 cctccgggga gctccggacc ggaggggcca aggatccacc ggtcgccacc atggtgagca
      361 agggcgagga gctgttcacc ggggtggtgc ccatcctggt cgagctggac ggcgacgtaa
      421 acggccacaa gttcagcgtg tccggcgagg gcgagggcga tgccacctac ggcaagctga
      481 ccctgaagtt catctgcacc accggcaagc tgcccgtgcc ctggcccacc ctcgtgacca
      541 ccttcggcta cggcctgcag tgcttcgccc gctaccccga ccacatgaag cagcacgact
      601 tcttcaagtc cgccatgccc gaaggctacg tccaggagcg caccatcttc ttcaaggacg
      661 acggcaacta caagacccgc gccgaggtga agttcgaggg cgacaccctg gtgaaccgca
      721 tcgagctgaa gggcatcgac ttcaaggagg acggcaacat cctggggcac aagctggagt
      781 acaactacaa cagccacaac gtctatatca tggccgacaa gcagaagaac ggcatcaagg
      841 tgaacttcaa gatccgccac aacatcgagg acggcagcgt gcagctcgcc gaccactacc
      901 agcagaacac ccccatcggc gacggccccg tgctgctgcc cgacaaccac tacctgagct
      961 accagtccgc cctgagcaaa gaccccaacg agaagcgcga tcacatggtc ctgctggagt
     1021 tcgtgaccgc cgccgggatc actctcggca tggacgagct gtacaagtaa agcggccgcg
     1081 actctagatc ataatcagcc ataccacatt tgtagaggtt ttacttgctt taaaaaacct
     1141 cccacacctc cccctgaacc tgaaaca
//
