LOCUS       sequence-8748-ex        9795 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9795)
  AUTHORS   Li QJ, Chau J, Ebert PJ, Sylvester G, Min H, Liu G, Braich R, 
            Manoharan M, Soutschek J, Skare P, Klein LO, Davis MM, Chen CZ
  TITLE     miR-181a is an intrinsic modulator of T cell sensitivity and 
            selection.
  JOURNAL   Cell. 2007 Apr 6. 129(1):147-61.
  PUBMED    17382377
REFERENCE   2  (bases 1 to 9795)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell. 2007 
            Apr 6. 129(1):147-61."
COMMENT     Sequence Label: MDH1-miR-181a1-SHP2
FEATURES             Location/Qualifiers
     source          1..9795
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    580..921
                     /label=MESV Psi
                     /note="packaging signal of murine embryonic stem cell 
                     virus"
     CDS             988..1404
                     /label=gag (truncated)
                     /note="truncated Moloney murine leukemia virus (MMLV) gag 
                     gene lacking the start codon"
     primer_bind     1334..1356
                     /label=pLXSN 5'
                     /note="Murine stem cell virus, forward primer. Also called 
                     MSCV"
     primer_bind     1372..1388
                     /label=pBABE 5'
                     /note="Psi packaging signal, forward primer for pBABE 
                     vectors"
     promoter        1422..1922
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     1855..1874
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     regulatory      3813..3822
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(3819..3821,3822..3824,3825..4538)
                     /codon_start=1
                     /product="enhanced GFP with monomerizing A206K mutation 
                     (Zacharias et al., 2002)"
                     /label=mEGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(3864..3885)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(4125..4144)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     4472..4493
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     misc_feature    4617..5078
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(4673..4690)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     4900..4919
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     promoter        complement(6000..6090)
                     /label=H1 promoter
                     /note="human H1 RNA promoter"
     primer_bind     complement(6060..6079)
                     /label=H1
                     /note="Human H1 promoter, forward primer"
     primer_bind     complement(6754..6770)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6754..6770)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(6767..6789)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    6778..6794
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(6802..6808,6809..6826,6827..6832))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6847..6868
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(6985..7002)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7156..7744)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7236..7255)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(7915..8706,8707..8775))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8538..8557
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8776..8880)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     8948..8966
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(9004..9026)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     9126..9145
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     9339..9361
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     9353..9370
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     9354..9370
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     9573..9592
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     LTR             join(9794..9795,1..516)
                     /label=5' LTR
                     /note="5' long terminal repeat from murine embryonic stem 
                     cell virus"
ORIGIN
        1 tgaaagaccc cacctgtagg tttggcaagc tagcttaagt aacgccattt tgcaaggcat
       61 ggaaaataca taactgagaa tagagaagtt cagatcaagg ttaggaacag agagacagca
      121 gaatatgggc caaacaggat atctgtggta agcagttcct gccccggctc agggccaaga
      181 acagatggtc cccagatgcg gtcccgccct cagcagtttc tagagaacca tcagatgttt
      241 ccagggtgcc ccaaggacct gaaaatgacc ctgtgcctta tttgaactaa ccaatcagtt
      301 cgcttctcgc ttctgttcgc gcgcttctgc tccccgagct caataaaaga gcccacaacc
      361 cctcactcgg cgcgccagtc ctccgataga ctgcgtcgcc cgggtacccg tattcccaat
      421 aaagcctctt gctgtttgca tccgaatcgt ggactcgctg atccttggga gggtctcctc
      481 agattgattg actgcccacc tcgggggtct ttcatttgga ggttccaccg agatttggag
      541 acccctgcct agggaccacc gacccccccg ccgggaggta agctggccag cggtcgtttc
      601 gtgtctgtct ctgtctttgt gcgtgtttgt gccggcatct aatgtttgcg cctgcgtctg
      661 tactagttag ctaactagct ctgtatctgg cggacccgtg gtggaactga cgagttctga
      721 acacccggcc gcaaccctgg gagacgtccc agggactttg ggggccgttt ttgtggcccg
      781 acctgaggaa gggagtcgat gtggaatccg accccgtcag gatatgtggt tctggtagga
      841 gacgagaacc taaaacagtt cccgcctccg tctgaatttt tgctttcggt ttggaaccga
      901 agccgcgcgt cttgtctgct gcagcgctgc agcatcgttc tgtgttgtct ctgtctgact
      961 gtgtttctgt atttgtctga aaattagggc cagactgtta ccactccctt aagtttgacc
     1021 ttaggtcact ggaaagatgt cgagcggatc gctcacaacc agtcggtaga tgtcaagaag
     1081 agacgttggg ttaccttctg ctctgcagaa tggccaacct ttaacgtcgg atggccgcga
     1141 gacggcacct ttaaccgaga cctcatcacc caggttaaga tcaaggtctt ttcacctggc
     1201 ccgcatggac acccagacca ggtcccctac atcgtgacct gggaagcctt ggcttttgac
     1261 ccccctccct gggtcaagcc ctttgtacac cctaagcctc cgcctcctct tcctccatcc
     1321 gccccgtctc tcccccttga acctcctcgt tcgaccccgc ctcgatcctc cctttatcca
     1381 gccctcactc cttctctagg cgccggaatt agatccctac cgggtagggg aggcgctttt
     1441 cccaaggcag tctggagcat gcgctttagc ggccccgctg ggcacttggc gctacacaag
     1501 tggcctctgg cctcgcacac attccacatc caccggtagg cgccaaccgg ctccgttctt
     1561 tggtggcccc ttcgcgccac cttctactcc tcccctagtc aggaagttcc cccccgcccc
     1621 gcagctcgcg tcgtgcagga cgtgacaaat ggaagtagca cgtctcacta gtctcgtgca
     1681 gatggacagc accgctgagc aatggaagcg ggtaggcctt tggggcagcg gccaatagca
     1741 gctttggctc cttcgctttc tgggctcaga ggctgggaag gggtgggtcc gggggcgggc
     1801 tcaggggcgg gctcaggggc ggggcgggcg cccgaaggtc ctccggaggc ccggcattct
     1861 gcacgcttca aaagcgcacg tctgccgcgc tgttctcctc ttcctcatct ccgggccttt
     1921 cgacctgcac tcgaagcggc cgcgttaacg tcgacagatc tacgcgtgcc accatgacat
     1981 cgcggagatg gtttcacccc aacatcactg gtgtggaggc agagaatctc ctgctgacca
     2041 gaggagtcga tggcagtttt ttagcaaggc ccagtaagag taaccctgga gacttcactc
     2101 tgtctgttag aagaaatgga gctgttaccc acatcaagat tcagaacact ggggactact
     2161 atgacctcta tggtggggag aagtttgcca ctttggctga actggttcag tattacatgg
     2221 aacaccatgg gcagctgaaa gagaagaatg gagatgttat cgagctcaag tacccgctga
     2281 actgtgcaga ccctacctct gaaaggtggt tccatggtca cttgtctgga aaagaagcag
     2341 agaagctgct gacggagaag ggcaagcatg gcagcttcct cgttcgagag agccagagcc
     2401 accccggaga cttcgttctc tccgtgcgca ctggtgacga caaaggggag agcaacgacg
     2461 gcaagtccaa agtgacccac gtcatgatcc gctgtcagga gctgaaatac gacgttggtg
     2521 ggggagagcg ctttgactct ctgacagacc tggtggagca ttacaagaag aaccccatgg
     2581 tggagacgct gggcacagtc ctgcagctca agcagcccct caacacaact cgtatcaatg
     2641 ctgctgaaat tgaaagccgg gttcgagagt taagcaagct ggctgagacc acagataaag
     2701 tcaagcaggg cttttgggaa gagtttgaga cgctccagca acaggaatgc aaacttctct
     2761 atagccgaaa agaaggacag agacaagaaa ataaaaacaa aaacagatac aaaaacatcc
     2821 tgccctttga tcataccagg gtcgttctgc atgatgggga tcccaatgag cctgtttctg
     2881 attacattaa tgcaaacatc atcatgcctg agtttgagac caagtgcaac aattccaaac
     2941 ccaaaaagag ttacattgcc actcaaggct gcctgcagaa cacggtgaat gacttctggc
     3001 ggatggtgtt ccaggagaac tctcgagtca ttgtcatgac cacaaaggaa gtggagagag
     3061 ggaagagcaa atgtgtcaag tactggcctg atgagtatgc gctcaaagaa tacggggtca
     3121 tgcgtgttag gaacgtcaaa gaaagtgccg cccatgacta cactttacga gagctcaaac
     3181 tctctaaggt cggacaagct ctactccagg gaaacacaga gagaaccgtc tggcagtacc
     3241 actttcggac ctggccagac catggcgtgc ctagtgaccc tggaggtgtg ctggacttcc
     3301 tggaggaggt ccaccacaag caggagagca tcgtggatgc aggccctgtc gtggttcact
     3361 gcagcgctgg gattggccgg acaggaacct tcattgtgat tgacatcctt attgacatca
     3421 ttcgagagaa aggtgtggac tgtgacatcg acgttcctaa aaccattcag atggtgcggt
     3481 cccagaggtc ggggatggtc cagacagaag cacagtaccg gtttatctac atggctgtcc
     3541 agcactacat agagacgctg cagcgccgga tcgaggagga gcagaaaagc aaaagaaaag
     3601 gacatgaata taccaatatt aagtattcct tggtggacca gacaagtggt gatcagagtc
     3661 ccctgccacc ctgcacccca acgccaccct gtgcagaaat gagggaggac agcgcccgag
     3721 tctatgagaa cgtgggcctc atgcagcagc agaggagttt cagaggggcc gcaggtggag
     3781 gcggatcagg tggcggtgga agtggaccgg tcgccaccat ggtgagcaag ggcgaggagc
     3841 tgttcaccgg ggtggtgccc atcctggtcg agctggacgg cgacgtaaac ggccacaagt
     3901 tcagcgtgtc cggcgagggc gagggcgatg ccacctacgg caagctgacc ctgaagttca
     3961 tctgcaccac cggcaagctg cccgtgccct ggcccaccct cgtgaccacc ctgacctacg
     4021 gcgtgcagtg cttcagccgc taccccgacc acatgaagca gcacgacttc ttcaagtccg
     4081 ccatgcccga aggctacgtc caggagcgca ccatcttctt caaggacgac ggcaactaca
     4141 agacccgcgc cgaggtgaag ttcgagggcg acaccctggt gaaccgcatc gagctgaagg
     4201 gcatcgactt caaggaggac ggcaacatcc tggggcacaa gctggagtac aactacaaca
     4261 gccacaacgt ctatatcatg gccgacaagc agaagaacgg catcaaggtg aacttcaaga
     4321 tccgccacaa catcgaggac ggcagcgtgc agctcgccga ccactaccag cagaacaccc
     4381 ccatcggcga cggccccgtg ctgctgcccg acaaccacta cctgagcacc cagtccaagc
     4441 tgagcaaaga ccccaacgag aagcgcgatc acatggtcct gctggagttc gtgaccgccg
     4501 ccgggatcac tctcggcatg gacgagctgt acaagtaatg aggatcaccg ggcaattgaa
     4561 gatctagtcg accaattccg gttattttcc accatattgc cgtcttttgg caatgtgagg
     4621 gcccggaaac ctggccctgt cttcttgacg agcattccta ggggtctttc ccctctcgcc
     4681 aaaggaatgc aaggtctgtt gaatgtcgtg aaggaagcag ttcctctgga agcttcttga
     4741 agacaaacaa cgtctgtagc gaccctttgc aggcagcgga accccccacc tggcgacagg
     4801 tgcctctgcg gccaaaagcc acgtgtataa gatacacctg caaaggcggc acaaccccag
     4861 tgccacgttg tgagttggat agttgtggaa agagtcaaat ggctctcctc aagcgtattc
     4921 aacaaggggc tgaaggatgc ccagaaggta ccccattgta tgggatctga tctggggcct
     4981 cggtgcacat gctttacatg tgtttagtcg aggttaaaaa acgtctaggc cccccgaacc
     5041 acggggacgt ggttttcctt tgaaaaacac gatgataata ccatgaaaac atttaacatt
     5101 tctcaacagg atctagaatt agtagaagta gcgacagaga agattacaat gctttatgag
     5161 gataataaac atcatgtggg agcggcaatt cgtacgaaaa caggagaaat catttcggca
     5221 gtacatattg aagcgtatat aggacgagta actgtttgtg cagaagccat tgcgattggt
     5281 agtgcagttt cgaatggaca aaaggatttt gacacgattg tagctgttag acacccttat
     5341 tctgacgaag tagatagaag tattcgagtg gtaagtcctt gtggtatgtg tagggagttg
     5401 atttcagact atgcaccaga ttgttttgtg ttaatagaaa tgaatggcaa gttagtcaaa
     5461 actacgattg aagaactcat tccactcaaa tatacccgaa attaatgagt taacttaatt
     5521 aagaattgcg gccgcgtcga cctgcagcca agcttatcga taaaataaaa gattttattt
     5581 agtctccaga aaaagggggg aatgaaagac cccacctgta ggtttggcaa gaattcgttt
     5641 tatcgataaa ataaaagatt ttatttagtc tccagaaaaa ggggggaatg aaagacccca
     5701 cctgtaggtt tggcaagaat tctaggaatt cttaattaaa aaagaatcct gtcgggtcct
     5761 gctttgcggt tctgtcttca gcaaacgcag agggccatgg agtagatgat ggttagctat
     5821 agggtacaat caacggtcga tggtttttat ttgaattcca aactcaccga cagcgttgaa
     5881 tgttcactga agcaacctgt gaggcagaga tgtgctgatt taaacagtat ctgtgggagt
     5941 ctggagcagg agggaggcag aggaaagccc aatcctttaa ccaaacctgt cacactcgag
     6001 tggtctcata cagaacttat aagattccca aatccaaaga catttcacgt ttatggtgat
     6061 ttcccagaac acatagcgac atgcaaatat tgggatccgc tagcttaagt aacgccattt
     6121 tgcaaggcat ggaaaataca taactgagaa tagagaagtt cagatcaagg ttaggaacag
     6181 agagacagca gaatatgggc caaacaggat atctgtggta agcagttcct gccccggctc
     6241 agggccaaga acagatggtc cccagatgcg gtcccgccct cagcagtttc tagagaacca
     6301 tcagatgttt ccagggtgcc ccaaggacct gaaaatgacc ctgtgcctta tttgaactaa
     6361 ccaatcagtt cgcttctcgc ttctgttcgc gcgcttctgc tccccgagct caataaaaga
     6421 gcccacaacc cctcactcgg cgcgccagtc ctccgataga ctgcgtcgcc cgggtacccg
     6481 tgtatccaat aaaccctctt gcagttgcat ccgacttgtg gtctcgctgt tccttgggag
     6541 ggtctcctct gagtgattga ctacccgtca gcgggggtct ttcatgggta acagtttctt
     6601 gaagttggag aacaacattc tgagggtagg agtcgaatat taagtaatcc tgactcaatt
     6661 agccactgtt ttgaatccac atactccaat actcctgaaa tagttcatta tggacagcgc
     6721 agaagagctg gggagaatta attcgtaatc atggtcatag ctgtttcctg tgtgaaattg
     6781 ttatccgctc acaattccac acaacatacg agccggaagc ataaagtgta aagcctgggg
     6841 tgcctaatga gtgagctaac tcacattaat tgcgttgcgc tcactgcccg ctttccagtc
     6901 gggaaacctg tcgtgccagc tgcattaatg aatcggccaa cgcgcgggga gaggcggttt
     6961 gcgtattggg cgctcttccg cttcctcgct cactgactcg ctgcgctcgg tcgttcggct
     7021 gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg ttatccacag aatcagggga
     7081 taacgcagga aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc
     7141 cgcgttgctg gcgtttttcc ataggctccg cccccctgac gagcatcaca aaaatcgacg
     7201 ctcaagtcag aggtggcgaa acccgacagg actataaaga taccaggcgt ttccccctgg
     7261 aagctccctc gtgcgctctc ctgttccgac cctgccgctt accggatacc tgtccgcctt
     7321 tctcccttcg ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc tcagttcggt
     7381 gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg
     7441 cgccttatcc ggtaactatc gtcttgagtc caacccggta agacacgact tatcgccact
     7501 ggcagcagcc actggtaaca ggattagcag agcgaggtat gtaggcggtg ctacagagtt
     7561 cttgaagtgg tggcctaact acggctacac tagaaggaca gtatttggta tctgcgctct
     7621 gctgaagcca gttaccttcg gaaaaagagt tggtagctct tgatccggca aacaaaccac
     7681 cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc
     7741 tcaagaagat cctttgatct tttctacggg gtctgacgct cagtggaacg aaaactcacg
     7801 ttaagggatt ttggtcatga gattatcaaa aaggatcttc acctagatcc ttttaaatta
     7861 aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa acttggtctg acagttacca
     7921 atgcttaatc agtgaggcac ctatctcagc gatctgtcta tttcgttcat ccatagttgc
     7981 ctgactcccc gtcgtgtaga taactacgat acgggagggc ttaccatctg gccccagtgc
     8041 tgcaatgata ccgcgagacc cacgctcacc ggctccagat ttatcagcaa taaaccagcc
     8101 agccggaagg gccgagcgca gaagtggtcc tgcaacttta tccgcctcca tccagtctat
     8161 taattgttgc cgggaagcta gagtaagtag ttcgccagtt aatagtttgc gcaacgttgt
     8221 tgccattgct acaggcatcg tggtgtcacg ctcgtcgttt ggtatggctt cattcagctc
     8281 cggttcccaa cgatcaaggc gagttacatg atcccccatg ttgtgcaaaa aagcggttag
     8341 ctccttcggt cctccgatcg ttgtcagaag taagttggcc gcagtgttat cactcatggt
     8401 tatggcagca ctgcataatt ctcttactgt catgccatcc gtaagatgct tttctgtgac
     8461 tggtgagtac tcaaccaagt cattctgaga atagtgtatg cggcgaccga gttgctcttg
     8521 cccggcgtca atacgggata ataccgcgcc acatagcaga actttaaaag tgctcatcat
     8581 tggaaaacgt tcttcggggc gaaaactctc aaggatctta ccgctgttga gatccagttc
     8641 gatgtaaccc actcgtgcac ccaactgatc ttcagcatct tttactttca ccagcgtttc
     8701 tgggtgagca aaaacaggaa ggcaaaatgc cgcaaaaaag ggaataaggg cgacacggaa
     8761 atgttgaata ctcatactct tcctttttca atattattga agcatttatc agggttattg
     8821 tctcatgagc ggatacatat ttgaatgtat ttagaaaaat aaacaaatag gggttccgcg
     8881 cacatttccc cgaaaagtgc cacctgacgt ctaagaaacc attattatca tgacattaac
     8941 ctataaaaat aggcgtatca cgaggccctt tcgtctcgcg cgtttcggtg atgacggtga
     9001 aaacctctga cacatgcagc tcccggagac ggtcacagct tgtctgtaag cggatgccgg
     9061 gagcagacaa gcccgtcagg gcgcgtcagc gggtgttggc gggtgtcggg gctggcttaa
     9121 ctatgcggca tcagagcaga ttgtactgag agtgcaccat atgcggtgtg aaataccgca
     9181 cagatgcgta aggagaaaat accgcatcag gcgccattcg ccattcaggc tgcgcaactg
     9241 ttgggaaggg cgatcggtgc gggcctcttc gctattacgc cagctggcga aagggggatg
     9301 tgctgcaagg cgattaagtt gggtaacgcc agggttttcc cagtcacgac gttgtaaaac
     9361 gacggccagt gccaagctcg ctctccctta tgcgactcct gcattaggaa gcagcccagt
     9421 agtaggttga ggccgttgag caccgccgcc gcaaggaatg gtgcatgcaa ggagatggcg
     9481 cccaacagtc ccccggccac ggggcctgcc accataccca cgccgaaaca agcgctcatg
     9541 agcccgaagt ggcgagcccg atcttcccca tcggtgatgt cggcgatata ggcgccagca
     9601 accgcacctg tggcgccggt gatgccggcc acgatgcgtc cggcgtagag gatcgattag
     9661 tccaatttgt taaagacagg atatcagtgg tccaggctct agttttgact caacaatatc
     9721 accagctgaa gcctatagag tacgagccat agataaaata aaagatttta tttagtctcc
     9781 agaaaaaggg gggaa
//
