LOCUS       sequence-87483-e        2089 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Lysosome Membrane, Excitation: 513, Emission: 527.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2089)
  TITLE     Davidson Yellow fluorescent proteins
REFERENCE   2  (bases 1 to 2089)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: EYFP-Lysosomes-20
FEATURES             Location/Qualifiers
     source          1..2089
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      42..51
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     regulatory      46..55
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(48..110,111..1268)
                     /codon_start=1
                     /product="rat lysosome-associated membrane glycoprotein 1"
                     /label=LAMP1
                     /translation="MAAPGARRPLLLLLLAGLAHSAPALFEVKDNNGTACIMASFSASF
                     LTTYEAGHVSKVSNMTLPASAEVLKNSSSCGEKNASEPTLAITFGEGYLLKLTFTKNTT
                     RYSVQHMYFTYNLSDTQFFPNASSKGPDTVDSTTDIKADINKTYRCVSDIRVYMKNVTI
                     VLWDATIQAYLPSSNFSKEETRCPQDQPSPTTGPPSPSPPLVPTNPSVSKYNVTGDNGT
                     CLLASMALQLNITYMKKDNTTVTRAFNINPSDKYSGTCGAQLVTLKVGNKSRVLELQFG
                     MNATSSLFFLQGVQLNMTLPDAIEPTFSTSNYSLKALQASVGNSYKCNSEEHIFVSKAL
                     ALNVFSVQVQAFRVESDRFGSVEECVQDGNNMLIPIAVGGALAGLVLIVLIAYLIGRKR
                     SHAGYQTI"
     CDS             join(1329..1331,1332..1334,1335..2048)
                     /codon_start=1
                     /product="enhanced YFP"
                     /label=EYFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTFGYGLQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSYQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(1374..1395)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(1635..1654)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     1982..2003
                     /label=EGFP-C
                     /note="EGFP, forward primer"
ORIGIN
        1 gacgtcagat ccgctagcgc taccggactc agatctcgag cgccaccatg gcggccccgg
       61 gcgcccggcg gccgctgctc ctgttgctgc tggcaggcct tgcacacagc gccccagcac
      121 tgttcgaggt gaaagacaac aacggcacag cgtgtataat ggccagcttc tctgcctcct
      181 ttctgaccac ctatgaggct ggacatgttt ctaaggtctc gaatatgacc ctgccagcct
      241 ctgcagaagt cctgaagaat agcagctctt gtggtgaaaa gaatgcttct gagcccaccc
      301 tcgcaatcac ctttggagaa ggatatttac tgaaactcac cttcacaaaa aacacaacac
      361 gttacagtgt ccagcacatg tatttcacat ataacctgtc agacacacaa ttctttccca
      421 atgccagctc caaagggccc gacactgtgg attccacaac tgacatcaag gcagacatca
      481 acaaaacata ccgatgtgtc agcgacatca gggtctacat gaagaatgtg accattgtgc
      541 tctgggacgc tactatccag gcctacctgc cgagtagcaa cttcagcaag gaagagacac
      601 gctgcccaca ggatcaacct tccccaacta ctgggccacc cagcccctca ccaccacttg
      661 tgcccacaaa ccccagtgtg tccaagtaca atgtgactgg tgacaatgga acctgcctgc
      721 tggcctctat ggcactgcaa ctcaacatca cctacatgaa gaaggacaac acgactgtga
      781 ccagagcatt caacatcaac ccaagtgaca aatatagtgg gacttgcggt gcccagttgg
      841 tgaccctgaa ggtggggaac aagagcagag tcctggagct gcagtttggg atgaatgcca
      901 cttctagcct gtttttcctg caaggagttc agttgaacat gactcttcct gatgccatag
      961 agcccacgtt cagcacctcc aactattccc tgaaagctct tcaggccagt gtcggcaact
     1021 catacaagtg caacagtgag gagcacatct ttgtcagcaa ggcgctcgcc ctcaatgtct
     1081 tcagcgtgca agtccaggct ttcagggtag aaagtgacag gtttgggtct gtggaagagt
     1141 gtgtacagga cggtaacaac atgctgatcc ccattgctgt gggcggggcc ctggcagggc
     1201 tggtcctcat cgtcctcatc gcctacctca tcggcaggaa gaggagtcac gcgggctatc
     1261 agaccatctc ggaattcggc tccaccggct ccaccggctc caccggcgcg gatccaccgg
     1321 tcgcccatat ggtgagcaag ggcgaggagc tgttcaccgg ggtggtgccc atcctggtcg
     1381 agctggacgg cgacgtaaac ggccacaagt tcagcgtgtc cggcgagggc gagggcgatg
     1441 ccacctacgg caagctgacc ctgaagttca tctgcaccac cggcaagctg cccgtgccct
     1501 ggcccaccct cgtgaccacc ttcggctacg gcctgcagtg cttcgcccgc taccccgacc
     1561 acatgaagca gcacgacttc ttcaagtccg ccatgcccga aggctacgtc caggagcgca
     1621 ccatcttctt caaggacgac ggcaactaca agacccgcgc cgaggtgaag ttcgagggcg
     1681 acaccctggt gaaccgcatc gagctgaagg gcatcgactt caaggaggac ggcaacatcc
     1741 tggggcacaa gctggagtac aactacaaca gccacaacgt ctatatcatg gccgacaagc
     1801 agaagaacgg catcaaggtg aacttcaaga tccgccacaa catcgaggac ggcagcgtgc
     1861 agctcgccga ccactaccag cagaacaccc ccatcggcga cggccccgtg ctgctgcccg
     1921 acaaccacta cctgagctac cagtccgccc tgagcaaaga ccccaacgag aagcgcgatc
     1981 acatggtcct gctggagttc gtgaccgccg ccgggatcac tctcggcatg gacgagctgt
     2041 acaagtaaag cggccgcgac tctagatcat aatcagccat accacattg
//
