LOCUS       sequence-87484-e        1043 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Mitochondria, Excitation: 513, Emission: 527.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1043)
  AUTHORS   Rizzo MA, Davidson MW, Piston DW
  TITLE     Fluorescent protein tracking and detection: fluorescent protein 
            structure and color variants.
  JOURNAL   Cold Spring Harb Protoc. 2009 Dec;2009(12):pdb.top63. doi: 
            10.1101/pdb.top63.
  PUBMED    20150100
REFERENCE   2  (bases 1 to 1043)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cold Spring
            Harb Protoc."; date: "2009-12"; volume: "2009(12):pdb.top63. doi"; 
            pages: " 10.1101/pdb.top63"
COMMENT     Sequence Label: EYFP-Mito-7
FEATURES             Location/Qualifiers
     source          1..1043
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        20..71
                     /label=CMVd2 promoter
                     /note="truncated human cytomegalovirus (CMV) immediate 
                     early promoter that yields moderately reduced expression 
                     relative to the full-length promoter"
     primer_bind     22..42
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     CDS             100..174
                     /codon_start=1
                     /product="mitochondrial presequence of human cytochrome c 
                     oxidase subunit VIII (Rizutto et al., 1989)"
                     /label=COX8 presequence
                     /translation="MSVLTPLLLRGLTGSARRLPVPRAK"
     regulatory      202..211
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(208..210,211..213,214..927)
                     /codon_start=1
                     /product="enhanced YFP"
                     /label=EYFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTFGYGLQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSYQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(253..274)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(514..533)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     861..882
                     /label=EGFP-C
                     /note="EGFP, forward primer"
ORIGIN
        1 gtaacaactc cgccccattg acgcaaatgg gcggtaggcg tgtacggtgg gaggtctata
       61 taagcagagc tggtttagtg aaccgtcaga tccgctagca tgtccgtcct gacgccgctg
      121 ctgctgcggg gcttgacagg ctcggcccgg cggctcccag tgccgcgcgc caagatccat
      181 tcgttggggg atccaccggt cgccaccatg gtgagcaagg gcgaggagct gttcaccggg
      241 gtggtgccca tcctggtcga gctggacggc gacgtaaacg gccacaagtt cagcgtgtcc
      301 ggcgagggcg agggcgatgc cacctacggc aagctgaccc tgaagttcat ctgcaccacc
      361 ggcaagctgc ccgtgccctg gcccaccctc gtgaccacct tcggctacgg cctgcagtgc
      421 ttcgcccgct accccgacca catgaagcag cacgacttct tcaagtccgc catgcccgaa
      481 ggctacgtcc aggagcgcac catcttcttc aaggacgacg gcaactacaa gacccgcgcc
      541 gaggtgaagt tcgagggcga caccctggtg aaccgcatcg agctgaaggg catcgacttc
      601 aaggaggacg gcaacatcct ggggcacaag ctggagtaca actacaacag ccacaacgtc
      661 tatatcatgg ccgacaagca gaagaacggc atcaaggtga acttcaagat ccgccacaac
      721 atcgaggacg gcagcgtgca gctcgccgac cactaccagc agaacacccc catcggcgac
      781 ggccccgtgc tgctgcccga caaccactac ctgagctacc agtccgccct gagcaaagac
      841 cccaacgaga agcgcgatca catggtcctg ctggagttcg tgaccgccgc cgggatcact
      901 ctcggcatgg acgagctgta caagtaaagc ggccgcgact ctagatcata atcagccata
      961 ccacatttgt agaggtttta cttgctttaa aaaacctccc acacctcccc ctgaacctga
     1021 aacataaaat gaatgcaatt gtt
//
