LOCUS       sequence-87491-e        4016 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Focal Adhesions, Excitation: 515, Emission: 528.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 4016)
  TITLE     Davidson Yellow fluorescent proteins
REFERENCE   2  (bases 1 to 4016)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: mVenus-Alpha-5-Integrin-12
FEATURES             Location/Qualifiers
     source          1..4016
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      3250..3259
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(3256..3258,3259..3261,3262..3975)
                     /codon_start=1
                     /product="Venus YFP with monomerizing A206K mutation (Nagai
                     et al., 2002; Kremers et al., 2006)"
                     /label=mVenus
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KLICTTGKLPVPWPTLVTTLGYGLQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYITADKQKNGIK
                     ANFKIRHNIEDGGVQLADHYQQNTPIGDGPVLLPDNHYLSYQSKLSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(3301..3322)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(3562..3581)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     3909..3930
                     /label=EGFP-C
                     /note="EGFP, forward primer"
ORIGIN
        1 gtcagatccg ctagcgctac cggactcaga tctcgagctc aagcttcgaa ttctgcagtc
       61 gacggtacca ccatggggag ccggacgcca gagtcccctc tccacgccgt gcagctgcgc
      121 tggggccccc ggcgccgacc cccgctgctg ccgctgctgt tgctgctgct gccgccgcca
      181 cccagggtcg ggggcttcaa cttagacgcg gaggccccag cagtactctc ggggcccccg
      241 ggctccttct tcggattctc agtggagttt taccggccgg gaacagacgg ggtcagtgtg
      301 ctggtgggag cacccaaggc taataccagc cagccaggag tgctgcaggg tggtgctgtc
      361 tacctctgtc cttggggtgc cagccccaca cagtgcaccc ccattgaatt tgacagcaaa
      421 ggctctcggc tcctggagtc ctcactgtcc agctcagagg gagaggagcc tgtggagtac
      481 aagtccttgc agtggttcgg ggcaacagtt cgagcccatg gctcctccat cttggcatgc
      541 gctccactgt acagctggcg cacagagaag gagccactga gcgaccccgt gggcacctgc
      601 tacctctcca cagataactt cacccgaatt ctggagtatg caccctgccg ctcagatttc
      661 agctgggcag caggacaggg ttactgccaa ggaggcttca gtgccgagtt caccaagact
      721 ggccgtgtgg ttttaggtgg accaggaagc tatttctggc aaggccagat cctgtctgcc
      781 actcaggagc agattgcaga atcttattac cccgagtacc tgatcaacct ggttcagggg
      841 cagctgcaga ctcgccaggc cagttccatc tatgatgaca gctacctagg atactctgtg
      901 gctgttggtg aattcagtgg tgatgacaca gaagactttg ttgctggtgt gcccaaaggg
      961 aacctcactt acggctatgt caccatcctt aatggctcag acattcgatc cctctacaac
     1021 ttctcagggg aacagatggc ctcctacttt ggctatgcag tggccgccac agacgtcaat
     1081 ggggacgggc tggatgactt gctggtgggg gcacccctgc tcatggatcg gacccctgac
     1141 gggcggcctc aggaggtggg cagggtctac gtctacctgc agcacccagc cggcatagag
     1201 cccacgccca cccttaccct cactggccat gatgagtttg gccgatttgg cagctccttg
     1261 acccccctgg gggacctgga ccaggatggc tacaatgatg tggccatcgg ggctcccttt
     1321 ggtggggaga cccagcaggg agtagtgttt gtatttcctg ggggcccagg agggctgggc
     1381 tctaagcctt cccaggttct gcagcccctg tgggcagcca gccacacccc agacttcttt
     1441 ggctctgccc ttcgaggagg ccgagacctg gatggcaatg gatatcctga tctgattgtg
     1501 gggtcctttg gtgtggacaa ggctgtggta tacaggggcc gccccatcgt gtccgctagt
     1561 gcctccctca ccatcttccc cgccatgttc aacccagagg agcggagctg cagcttagag
     1621 gggaaccctg tggcctgcat caaccttagc ttctgcctca atgcttctgg aaaacacgtt
     1681 gctgactcca ttggtttcac agtggaactt cagctggact ggcagaagca gaagggaggg
     1741 gtacggcggg cactgttcct ggcctccagg caggcaaccc tgacccagac cctgctcatc
     1801 cagaatgggg ctcgagagga ttgcagagag atgaagatct acctcaggaa cgagtcagaa
     1861 tttcgagaca aactctcgcc gattcacatc gctctcaact tctccttgga cccccaagcc
     1921 ccagtggaca gccacggcct caggccagcc ctacattatc agagcaagag ccggatagag
     1981 gacaaggctc agatcttgct ggactgtgga gaagacaaca tctgtgtgcc tgacctgcag
     2041 ctggaagtgt ttggggagca gaaccatgtg tacctgggtg acaagaatgc cctgaacctc
     2101 actttccatg cccagaatgt gggtgagggt ggcgcctatg aggctgagct tcgggtcacc
     2161 gcccctccag aggctgagta ctcaggactc gtcagacacc cagggaactt ctccagcctg
     2221 agctgtgact actttgccgt gaaccagagc cgcctgctgg tgtgtgacct gggcaacccc
     2281 atgaaggcag gagccagtct gtggggtggc cttcggttta cagtccctca tctccgggac
     2341 actaagaaaa ccatccagtt tgacttccag atcctcagca agaatctcaa caactcgcaa
     2401 agcgacgtgg tttcctttcg gctctccgtg gaggctcagg cccaggtcac cctgaacggt
     2461 gtctccaagc ctgaggcagt gctattccca gtaagcgact ggcatccccg agaccagcct
     2521 cagaaggagg aggacctggg acctgctgtc caccatgtct atgagctcat caaccaaggc
     2581 cccagctcca ttagccaggg tgtgctggaa ctcagctgtc cccaggctct ggaaggtcag
     2641 cagctcctat atgtgaccag agttacggga ctcaactgca ccaccaatca ccccattaac
     2701 ccaaagggcc tggagttgga tcccgagggt tccctgcacc accagcaaaa acgggaagct
     2761 ccaagccgca gctctgcttc ctcgggacct cagatcctga aatgcccgga ggctgagtgt
     2821 ttcaggctgc gctgtgagct cgggcccctg caccaacaag agagccaaag tctgcagttg
     2881 catttccgag tctgggccaa gactttcttg cagcgggagc accagccatt tagcctgcag
     2941 tgtgaggctg tgtacaaagc cctgaagatg ccctaccgaa tcctgcctcg gcagctgccc
     3001 caaaaagagc gtcaggtggc cacagctgtg caatggacca aggcagaagg cagctatggc
     3061 gtcccactgt ggatcatcat cctagccatc ctgtttggcc tcctgctcct aggtctactc
     3121 atctacatcc tctacaagct tggattcttc aaacgctccc tcccatatgg caccgccatg
     3181 gaaaaagctc agctcaagcc tccagccacc tctgatgcca cggtaccgcg ggcccgggat
     3241 ccaccggtcg ccaccatggt gagcaagggc gaggagctgt tcaccggggt ggtgcccatc
     3301 ctggtcgagc tggacggcga cgtaaacggc cacaagttca gcgtgtccgg cgagggcgag
     3361 ggcgatgcca cctacggcaa gctgaccctg aagctgatct gcaccaccgg caagctgccc
     3421 gtgccctggc ccaccctcgt gaccaccctg ggctacggcc tgcagtgctt cgcccgctac
     3481 cccgaccaca tgaagcagca cgacttcttc aagtccgcca tgcccgaagg ctacgtccag
     3541 gagcgcacca tcttcttcaa ggacgacggc aactacaaga cccgcgccga ggtgaagttc
     3601 gagggcgaca ccctggtgaa ccgcatcgag ctgaagggca tcgacttcaa ggaggacggc
     3661 aacatcctgg ggcacaagct ggagtacaac tacaacagcc acaacgtcta tatcaccgcc
     3721 gacaagcaga agaacggcat caaggccaac ttcaagatcc gccacaacat cgaggacggc
     3781 ggcgtgcagc tcgccgacca ctaccagcag aacaccccca tcggcgacgg ccccgtgctg
     3841 ctgcccgaca accactacct gagctaccag tccaagctga gcaaagaccc caacgagaag
     3901 cgcgatcaca tggtcctgct ggagttcgtg accgccgccg ggatcactct cggcatggac
     3961 gagctgtaca agtaaagcgg ccgcgactct agatcataat cagccatacc acattt
//
