LOCUS       sequence-87497-e        1749 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Gap Junctions, Excitation: 515, Emission: 528.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1749)
  TITLE     Davidson Yellow fluorescent proteins
REFERENCE   2  (bases 1 to 1749)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: mVenus-Cx32-7
FEATURES             Location/Qualifiers
     source          1..1749
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      983..992
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(989..991,992..994,995..1708)
                     /codon_start=1
                     /product="Venus YFP with monomerizing A206K mutation (Nagai
                     et al., 2002; Kremers et al., 2006)"
                     /label=mVenus
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KLICTTGKLPVPWPTLVTTLGYGLQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYITADKQKNGIK
                     ANFKIRHNIEDGGVQLADHYQQNTPIGDGPVLLPDNHYLSYQSKLSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(1034..1055)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(1295..1314)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     1642..1663
                     /label=EGFP-C
                     /note="EGFP, forward primer"
ORIGIN
        1 gacgtcagat ccgctagcgc taccggactc agatctcgag ctcaagcttc gaattccctc
       61 tgggaaaggg cagcagcagc caggtgtggc agtgacaggg aggtgtgaat gaggcaggat
      121 gaactggaca ggtttgtaca ccttgctcag tggcgtgaac cggcattcta ctgccattgg
      181 ccgagtatgg ctctcggtca tcttcatctt cagaatcatg gtgctggtgg tggctgcaga
      241 gagtgtgtgg ggtgatgaga aatcttcctt catctgcaac acactccagc ctggctgcaa
      301 cagcgtttgc tatgaccaat tcttccccat ctcccatgtg cggctgtggt ccctgcagct
      361 catcctagtt tccaccccag ctctcctcgt ggccatgcac gtggctcacc agcaacacat
      421 agagaagaaa atgctacggc ttgagggcca tggggacccc ctacacctgg aggaggtgaa
      481 gaggcacaag gtccacatct cagggacact gtggtggacc tatgtcatca gcgtggtgtt
      541 ccggctgttg tttgaggccg tcttcatgta tgtcttttat ctgctctacc ctggctatgc
      601 catggtgcgg ctggtcaagt gcgacgtcta cccctgcccc aacacagtgg actgcttcgt
      661 gtcccgcccc accgagaaaa ccgtcttcac cgtcttcatg ctagctgcct ctggcatctg
      721 catcatcctc aatgtggccg aggtggtgta cctcatcatc cgggcctgtg cccgccgagc
      781 ccagcgccgc tccaatccac cttcccgcaa gggctcgggc ttcggccacc gcctctcacc
      841 tgaatacaag cagaatgaga tcaacaagct gctgagtgag caggatggct ccctgaaaga
      901 catactgcgc cgcagccctg gcaccggggc tgggctggct gaaaagagcg accgctgctc
      961 ggcctgcgcg gatccaccgg tcgccaccat ggtgagcaag ggcgaggagc tgttcaccgg
     1021 ggtggtgccc atcctggtcg agctggacgg cgacgtaaac ggccacaagt tcagcgtgtc
     1081 cggcgagggc gagggcgatg ccacctacgg caagctgacc ctgaagctga tctgcaccac
     1141 cggcaagctg cccgtgccct ggcccaccct cgtgaccacc ctgggctacg gcctgcagtg
     1201 cttcgcccgc taccccgacc acatgaagca gcacgacttc ttcaagtccg ccatgcccga
     1261 aggctacgtc caggagcgca ccatcttctt caaggacgac ggcaactaca agacccgcgc
     1321 cgaggtgaag ttcgagggcg acaccctggt gaaccgcatc gagctgaagg gcatcgactt
     1381 caaggaggac ggcaacatcc tggggcacaa gctggagtac aactacaaca gccacaacgt
     1441 ctatatcacc gccgacaagc agaagaacgg catcaaggcc aacttcaaga tccgccacaa
     1501 catcgaggac ggcggcgtgc agctcgccga ccactaccag cagaacaccc ccatcggcga
     1561 cggccccgtg ctgctgcccg acaaccacta cctgagctac cagtccaagc tgagcaaaga
     1621 ccccaacgag aagcgcgatc acatggtcct gctggagttc gtgaccgccg ccgggatcac
     1681 tctcggcatg gacgagctgt acaagtaaag cggccgcgac tctagatcat aatcagccat
     1741 accacattg
//
