LOCUS       sequence-87501-e        1775 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Nucleoli, Excitation: 515, Emission: 528.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1775)
  TITLE     Davidson Yellow fluorescent proteins
REFERENCE   2  (bases 1 to 1775)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: mVenus-Fibrillarin-7
FEATURES             Location/Qualifiers
     source          1..1775
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      28..37
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(34..36,37..39,40..750)
                     /codon_start=1
                     /product="Venus YFP with monomerizing A206K mutation (Nagai
                     et al., 2002; Kremers et al., 2006)"
                     /label=mVenus
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KLICTTGKLPVPWPTLVTTLGYGLQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYITADKQKNGIK
                     ANFKIRHNIEDGGVQLADHYQQNTPIGDGPVLLPDNHYLSYQSKLSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(79..100)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(340..359)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     687..708
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     CDS             complement(1546..1557)
                     /codon_start=1
                     /gene="cspA"
                     /product="translation enhancing element for E. coli (Qing 
                     et al., 2004)"
                     /label=TEE
                     /translation="NHKV"
ORIGIN
        1 acgtcagatc cgctagcgct accggtcgcc accatggtga gcaagggcga ggagctgttc
       61 accggggtgg tgcccatcct ggtcgagctg gacggcgacg taaacggcca caagttcagc
      121 gtgtccggcg agggcgaggg cgatgccacc tacggcaagc tgaccctgaa gctgatctgc
      181 accaccggca agctgcccgt gccctggccc accctcgtga ccaccctggg ctacggcctg
      241 cagtgcttcg cccgctaccc cgaccacatg aagcagcacg acttcttcaa gtccgccatg
      301 cccgaaggct acgtccagga gcgcaccatc ttcttcaagg acgacggcaa ctacaagacc
      361 cgcgccgagg tgaagttcga gggcgacacc ctggtgaacc gcatcgagct gaagggcatc
      421 gacttcaagg aggacggcaa catcctgggg cacaagctgg agtacaacta caacagccac
      481 aacgtctata tcaccgccga caagcagaag aacggcatca aggccaactt caagatccgc
      541 cacaacatcg aggacggcgg cgtgcagctc gccgaccact accagcagaa cacccccatc
      601 ggcgacggcc ccgtgctgct gcccgacaac cactacctga gctaccagtc caagctgagc
      661 aaagacccca acgagaagcg cgatcacatg gtcctgctgg agttcgtgac cgccgccggg
      721 atcactctcg gcatggacga gctgtacaag tccggactca gatctcgagc catgaagcca
      781 ggattcagtc cccgtggggg tggctttggc ggccgagggg gctttggtga ccgtggtggt
      841 cgtggaggcc gagggggctt tggcgggggc cgaggccaag gcggaggctt tagaggtcgt
      901 ggacgaggag gaggtggagg cggcggcggc ggtggaggag gaggaagagg tggtggaggc
      961 ttccattttg gtggcaaccg gggtcgtggt cggggaggaa aaagaggaaa ccagtcgggg
     1021 aagaatgtga tggtggagcc gcatcggcat gagggtgtct tcatttgtcg gggaaaggaa
     1081 gatgcactgg tcaccaagaa cctggtccct ggggaatcag tttatggaga gaagagagtc
     1141 tcgatttcgg aaggagatga caaaattgag taccgagcct ggaacccctt ccgctccaag
     1201 cttgcagcag caatcctggg tggtgtggac cagatccaca tcaaaccggg ggctaaggtt
     1261 ctctacctcg gggctgcctc gggcaccacg gtctcccatg tctctgacat cgttggtccg
     1321 gatggtctag tctatgcagt cgagttctcc caccgctctg gccgtgacct cattaacttg
     1381 gccaagaaga ggaccaacat cattcctgtg atcgaggatg ctcgacaccc acacaaatac
     1441 cgcatgctca tcgcaatggt ggatgtgatc tttgctgatg tggcccagcc agaccagacc
     1501 cggattgtgg ccctgaatgc ccacaccttc ctgcgtaatg gaggacactt tgtgatttcc
     1561 attaaggcca actgcattga ctccacagcc tcagccgagg ccgtgtttgc ctccgaagtg
     1621 aaaaagatgc aacaggagaa catgaagccg caggagcagt tgacccttga gccatatgaa
     1681 agagaccatg ccgtggtcgt gggagtgtac aggccacccc ccaaggtgaa gaactgagga
     1741 tccaccggat ctagataact gatcataatc agcca
//
