LOCUS       sequence-87510-e        8937 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Focal Adhesions, Excitation: 515, Emission: 528.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 8937)
  TITLE     Davidson Yellow fluorescent proteins
REFERENCE   2  (bases 1 to 8937)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: mVenus-Talin-18
FEATURES             Location/Qualifiers
     source          1..8937
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      30..39
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(36..38,39..41,42..752)
                     /codon_start=1
                     /product="Venus YFP with monomerizing A206K mutation (Nagai
                     et al., 2002; Kremers et al., 2006)"
                     /label=mVenus
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KLICTTGKLPVPWPTLVTTLGYGLQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYITADKQKNGIK
                     ANFKIRHNIEDGGVQLADHYQQNTPIGDGPVLLPDNHYLSYQSKLSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(81..102)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(342..361)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     689..710
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     regulatory      801..810
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 tgacgtcaga tccgctagcg ctaccggtcg ccaccatggt gagcaagggc gaggagctgt
       61 tcaccggggt ggtgcccatc ctggtcgagc tggacggcga cgtaaacggc cacaagttca
      121 gcgtgtccgg cgagggcgag ggcgatgcca cctacggcaa gctgaccctg aagctgatct
      181 gcaccaccgg caagctgccc gtgccctggc ccaccctcgt gaccaccctg ggctacggcc
      241 tgcagtgctt cgcccgctac cccgaccaca tgaagcagca cgacttcttc aagtccgcca
      301 tgcccgaagg ctacgtccag gagcgcacca tcttcttcaa ggacgacggc aactacaaga
      361 cccgcgccga ggtgaagttc gagggcgaca ccctggtgaa ccgcatcgag ctgaagggca
      421 tcgacttcaa ggaggacggc aacatcctgg ggcacaagct ggagtacaac tacaacagcc
      481 acaacgtcta tatcaccgcc gacaagcaga agaacggcat caaggccaac ttcaagatcc
      541 gccacaacat cgaggacggc ggcgtgcagc tcgccgacca ctaccagcag aacaccccca
      601 tcggcgacgg ccccgtgctg ctgcccgaca accactacct gagctaccag tccaaactga
      661 gcaaagaccc caacgagaag cgcgatcaca tggtcctgct ggagttcgtg accgccgccg
      721 ggatcactct cggcatggac gagctgtaca agtccggact cagatctcga gctcaagcta
      781 gcttcgaatt cgcagaagct gccaccatgg ttgcgctttc gctgaagatt agcattggga
      841 atgtggtgaa gacgatgcaa tttgagccat ctaccatggt gtacgacgcc tgccgcatga
      901 ttcgtgagcg gatcccagag gccctggctg gccctcccaa cgactttggg ctctttctgt
      961 cagatgatga ccccaaaaaa ggcatctggc tagaggctgg gaaagctttg gactactaca
     1021 tgctccgaaa tggggacacc atggagtaca gaaaaaagca gagacccctg aagatacgga
     1081 tgttagacgg aacggtgaag actatcatgg tggatgactc caagaccgtc acagacatgc
     1141 tcatgaccat ttgtgcccga attggtatca ccaaccatga tgagtattca ctggttcgag
     1201 agctgatgga agaaaagaaa gatgagggga cagggaccct gagaaaagac aagaccctac
     1261 tgcgagatga aaagaagatg gagaagctaa agcagaagtt gcacacagac gatgagttga
     1321 actggctgga ccatgggcgg acactgaggg aacagggagt ggaagagcat gagacgttgc
     1381 tgctgcggag aaagttcttc tactcagacc agaatgtgga ttcccgagac cctgtacagc
     1441 tgaaccttct ctatgtgcag gcacgagatg acatcctgaa tggctcccat cctgtctcct
     1501 ttgacaaggc ctgtgaattt gcaggcttcc agtgccagat ccagtttgga cctcacaatg
     1561 aacagaagca caaggctggc ttccttgacc tgaaggactt cctgcccaag gagtatgtga
     1621 agcagaaggg agagcgtaag atctttcagg cacacaagaa ttgtgggcag atgagtgaaa
     1681 ttgaggccaa ggtacgctac gtgaagctgg cccgttccct caagacttac ggtgtctcct
     1741 tcttcctagt caaggaaaag atgaagggga agaataaact ggtgcccagg ctgttgggca
     1801 tcactaagga gtgtgtgatg cgtgtggatg agaagaccaa ggaggtgatt caggaatgga
     1861 gcctcaccaa catcaaacgc tgggctgcct ctcccaagag ctttactctg gactttggag
     1921 actatcagga tggctactac tcagtacaga cgacagaagg cgagcagatc gcacagctca
     1981 ttgctggcta catagatatc atccttaaga agaaaaaaag caaggaccat tttgggctgg
     2041 agggagatga agagtctact atgttggagg actcagtgtc tcccaaaaag tcaacagtcc
     2101 ttcagcagca gtacaaccga gtggggaaag tggagcacgg ctctgtggct ctgccagcca
     2161 tcatgcgctc tggagcctct ggtcctgaga atttccaggt ggggagcatg ccacctgccc
     2221 agcagcagat caccagtggc cagatgcacc gaggacacat gccacctctg acttcagccc
     2281 agcaggcgct cactggaacc attaactcca gcatgcaggc tgtgcaggct gcccaggcca
     2341 ctctggatga ctttgagact ctaccccctc ttggccagga tgctgcctcc aaagcttggc
     2401 gtaagaacaa gatggatgaa tcaaagcatg agatccactc ccaggtagat gccatcacag
     2461 ctggcactgc ctctgtggta aacctgacag caggagatcc tgcagagaca gactatacag
     2521 cagtgggctg tgcagtcacc accatctctt ccaacctgac ggagatgtcc cgtggggtga
     2581 agctattggc tgccctgctg gaggatgaag gcggcaatgg ccggcccctt ctgcaagcag
     2641 caaagggcct tgcaggggct gtgtcggaac tgcttcgcag tgctcagccc gccagcgctg
     2701 agcctcgtca gaacctgctg caggcagccg ggaacgtggg ccaggccagt ggggagctgt
     2761 tgcagcaaat tggggaaagt gacactgacc cccacttcca ggatgttcta atgcagctag
     2821 caaacgcagt ggcaagtgct gcagccgccc tggtcctcaa ggccaagagt gtggcccagc
     2881 gcacagagga ttccgggctt cagacccaag tgattgctgc agctacgcaa tgtgctctgt
     2941 ccacttccca gctggtggcc tgtaccaagg tggtagcacc tacaatcagc tcacctgtgt
     3001 gccaagaaca gctagtcgag gctgggcgac tggtggccaa agctgtggag ggctgtgtgt
     3061 ctgcctcgca agcagctaca gaagatggac agctgcttcg aggggtagga gcagcagcca
     3121 cggctgtcac ccaggccctc aatgagctgc tgcagcacgt gaaggcccac gccaccggag
     3181 ctgggcctgc cggtcggtat gaccaagcca ctgataccat cctcaccgtc actgaaaaca
     3241 tcttcagctc catgggtgat gctggggaga tggtgcgcca ggcccgcatc ctggcccaag
     3301 ccacctcaga cctggtcaat gctatcaagg ccgatgctga gggggagagt gatctggaga
     3361 actctagaaa gctcctgagt gctgccaaga tcctcgctga tgccaccgcc aagatggtgg
     3421 aggcggccaa gggagcagcc gcccaccctg acagtgagga acagcagcag cgactgcgtg
     3481 aagcagctga ggggcttcgc atggccacca atgcagctgc gcagaacgcc atcaagaaga
     3541 agttggtgca gcgcctggag catgcagcca agcaagctgc agcctctgca acacagacca
     3601 ttgctgcagc ccaacatgca gcctctgccc ccaaggcctc tgcgggcccc cagcccctac
     3661 tggtgcagag ctgtaaggcc gtggcagagc agattccgtt gctggtgcag ggtgtccgag
     3721 gaagtcaagc tcagcctgac agccctagtg ctcagctcgc cctcattgct gccagccaga
     3781 gcttcctgca gccaggtggg aagatggtgg cagcggcaaa agcctcagtg ccaacaattc
     3841 aggaccaggc ttcagcgatg cagctgagtc agtgcgcaaa gaacctaggc actgccctgg
     3901 ccgagctgcg cactgctgct cagaaggctc aggaggcatg tggacctttg gagatggatt
     3961 ctgcactgag tgttgtacaa aatctagaga aagacctaca ggaaataaaa gcagcagctc
     4021 gagatggcaa gcttaagccc ttacctgggg aaacaatgga gaagtgtacc caagatcttg
     4081 gcaacagcac caaagcggtg agctctgcca tcgccaagct gctgggggag attgcccagg
     4141 gcaacgagaa ctatgcaggt attgcagctc gggatgtagc tggtggacta aggtcactag
     4201 cccaggctgc gcggggtgtg gctgcgctga catcagatcc tgcagtgcag gccattgtgc
     4261 ttgacacagc cagcgacgtt ctggacaagg ccagcagcct cattgaagag gcaaaaaaag
     4321 catctggaca cccaggggac ccagaaagcc agcaaaggct tgctcaggta gctaaagcag
     4381 tgacccaagc cctgaaccgc tgtgtcagct gcctgcctgg ccaaagagac gtggataatg
     4441 ccctacgagc cgttggagat gccagcaagc gactcctgag tgacttgctt cctccaagca
     4501 cggggacatt tcaagaagca cagagccgat tgaatgaagc tgcagctggg ttaaatcagg
     4561 ccgccacaga actggtgcaa gcctcccgag gaactcctca ggacctggct cgggcctccg
     4621 gccgatttgg acaggacttc agcaccttcc tggaagctgg tgtggagatg gccggacagg
     4681 ccccgagcca ggaggaccga gcccaggtgg tgtccaacct gaagggcata tccatgtctt
     4741 caagcaaact tctccttgcc gctaaggcct tgtccacaga ccctgcttct cccaacctca
     4801 agagtcagct ggctgcggct gcccgggcag tgacggacag catcaaccag ctcatcacca
     4861 tgtgcaccca gcaggcacct ggccagaagg agtgtgacaa tgcacttcgg cagctagaga
     4921 cagtccgaga actcctggag aatccagtcc agcccatcaa cgacatgtcc tacttcggtt
     4981 gcttggacag tgtcatggag aactccaagg tcctaggtga ggccatgact ggcatctccc
     5041 aaaatgccaa gaatggaaat ctgccggagt ttggagatgc cattgccaca gcctccaagg
     5101 ctctctgtgg cttcacggag gcagccgcac aggcagcata tctagttggt gtctctgacc
     5161 ccaacagcca agctggacag caaggactgg tggaacccac acagtttgcc cgtgcaaacc
     5221 aggcaattca gatggcctgt cagagtctgg gggagcctgg ctgtacccag gcccaggtgt
     5281 tatctgcagc cactattgta gccaaacaca catctgcatt gtgtaacagc tgtcgcctgg
     5341 cttccgctag gactgccaat cccactgcca agcgccagtt tgtacagtca gccaaggagg
     5401 tggccaacag tacagccaat cttgtcaaga ccatcaaggc actagatggg gacttcacag
     5461 aagagaaccg tgcccagtgc cgagcagcca cagcccctct gctggaagct gtggataacc
     5521 tgagtgcctt tgcctccaac cctgagttct ccagcgtccc tgcccagatc agccctgagg
     5581 gcagagcagc catggagccc attgtaatct ctgctaagac aatgttggag agtgctggag
     5641 ggctcatcca gacagctcgg gcgttagcag tcaatccccg agacccccca cgttggtctg
     5701 tgctagctgg ccactctcgg actgtctcag attccatcaa gaaacttatt acaagcatga
     5761 gggacaaagc cccagggcag ctggagtgtg agacagccat tgcggctctg aacagctgct
     5821 tgcgggacct agatcaggct tcgcttgccg ctgtcagcca gcagcttgct ccccgtgaag
     5881 gaatctctca agaggctttg cacacccaga tgctcactgc agtgcaagaa atttctcatc
     5941 tcattgagcc gctggccagc gctgctcggg ctgaagcctc ccagctggga cacaaggtgt
     6001 cccaaatggc ccagtacttt gaaccactca ccctggctgc agtgggtgct gcgtctaaga
     6061 ccctgagcca cccgcagcag atggcacttc tggaccagac taaaacgttg gcagagtctg
     6121 ccttgcagtt gctatatact gccaaggagg ctggtggtaa ccccaagcaa gcagcacaca
     6181 cccaggaagc cctggaggag gctgtgcaga tgatgacaga ggccgtagaa gacctgacga
     6241 cgaccctcaa cgaagcagcc agtgctgcag gagtcgttgg cggcatggtg gactctatca
     6301 ctcaggccat caaccagcta gatgaaggac ctatgggtga cccagaaggc tcattcgtag
     6361 attaccagac aaccatggtg aggacagcca aggccattgc tgtcactgtt caggagatgg
     6421 taaccaagtc aaacaccagc cctgaagagc taggccctct tgccaaccag ctgaccagtg
     6481 actatggccg actggcctca caagccaagc ctgcagctgt ggctgctgaa aatgaagaga
     6541 taggcgctca catcaaacac cgagtacagg agctgggcca tggctgctct gctctggtca
     6601 ccaaggcagg tgccttgcag tgtagcccca gtgatgtcta caccaagaag gagctcatag
     6661 agtgtgcccg cagagtgtca gagaaggtct cccatgttct ggctgcactc caggctggga
     6721 atcgtggtac ccaggcctgc attacagcag ccagtgctgt gtctggtatc attgctgacc
     6781 tcgacaccac catcatgttc gctactgctg gcacacttaa ccgtgagggt gctgaaactt
     6841 ttgctgacca ccgggagggt atcttaaaga cagcaaaggt tcttgtggag gacaccaagg
     6901 tcctagtgca gaatgcagct gggagccagg agaagttggc acaagccgcc cagtcctccg
     6961 tggccaccat tacccgcctc gctgatgtgg tcaagctcgg tgcagccagc ctaggagccg
     7021 aagaccctga aactcaggtg gtgctgatca atgcagtaaa ggacgtagcc aaggccctgg
     7081 gtgacctcat cagcgctacg aaggctgcag cgggcaaagt tggggatgac cctgcagtgt
     7141 ggcagctcaa gaactctgcc aaggtgatgg tgaccaatgt gacatcattg ctcaagacag
     7201 tgaaggctgt ggaagatgag gccaccaaag gcacacgggc cctagaggca accacagagc
     7261 acatacgtca ggaactggcg gtcttctgtt ccccagagcc acctgccaag acctctaccc
     7321 ctgaagactt catccgaatg accaagggta ttactatggc aacagccaaa gccgttgctg
     7381 ctggcaattc ctgtcgacag gaagatgtca ttgccacagc caatctgagc cgacgggcta
     7441 ttgcggacat gcttcgggct tgcaaggaag cagctttcca cccagaagtg gcgcctgatg
     7501 tacggctccg agccctgcac tatggccggg agtgtgccaa tggttacctg gaactgctgg
     7561 accacgtgct gctgaccctt cagaagccaa acccagacct gaagcagcag ctaactggac
     7621 actcaaagcg ggttgctggc tctgtgactg agctcatcca ggctgctgaa gccatgaaag
     7681 gaacagagtg ggtggaccca gaggacccta ctgtcattgc tgagaatgag ctcttgggag
     7741 ccgcagccgc catcgaggct gcagccaaga agctggagca gctgaagccc agggccaaac
     7801 ccaaggaggc ggatgagtcc ttgaactttg aggaacaaat cctagaagct gccaagtcca
     7861 tcgctgcagc caccagtgca ctggtaaagg ctgcgtcagc agcccagagg gagctggtgg
     7921 ctcaaggaaa ggtgggcgcc attccagcca atgcactgga tgatgggcag tggtcgcagg
     7981 gcctcatttc cgctgcccgt atggtggctg cggccaccaa caatctgtgt gaagcagcta
     8041 acgcagctgt ccagggccat gctagccagg agaaactcat ttcctcagcc aagcaagtag
     8101 ctgcctccac agctcagctc ctggtagctt gcaaggtcaa agcagatcag gactctgagg
     8161 caatgaaacg gctccaggct gctggcaatg cagtgaagag ggcctcagat aacctggtaa
     8221 aggcggccca gaaagctgca gccttcgaag accaggagaa tgagacggtg gtggtgaagg
     8281 agaaaatggt tgggggcatt gcccagatta tcgcagcaca ggaagagatg cttcggaagg
     8341 aacgagagct ggaagaggct cggaaaaagc tcgcccagat ccggcagcag cagtacaagt
     8401 tcttgccttc agagcttcga gacgagcact aaaaaagccc tgtgtattta atgcagaccc
     8461 agcccagaga ctgtgcctgc cactaccaaa gccttctggg ctgctcagga cccaagctgc
     8521 ccaaccccag cactccccca aagtgcctgc caaaccctgg gcctggccct gcccagtccc
     8581 actgcaagcc gtgtcctctc cctgactccc aagtgccttt gcaccctagg gcccctaagt
     8641 gcctgccccc ctccagagta ttaacgctcc aagagtatta ttaatgctgc cgtacctcag
     8701 tttgaacctg ccagggcccc agctgctcca gcctgccagc agcttccagc cagtccccac
     8761 ggccacgtca gctcaactca tccctttttg atactatatc ccctacccag ctacctatgg
     8821 ggcttgaggg ttgtaaaccc aaacaggtca gactcccgga attctgcagt cgacggtacc
     8881 gcgggcccgg gatccaccgg atctagataa ctgatcataa tcagccatac cacattg
//
