LOCUS       sequence-87512-e        1917 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Actin/Focal Adhesions, Excitation: 515, Emission: 528.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1917)
  TITLE     Davidson Yellow fluorescent proteins
REFERENCE   2  (bases 1 to 1917)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: mVenus-VASP-5
FEATURES             Location/Qualifiers
     source          1..1917
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      26..35
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(32..34,35..37,38..748)
                     /codon_start=1
                     /product="Venus YFP with monomerizing A206K mutation (Nagai
                     et al., 2002; Kremers et al., 2006)"
                     /label=mVenus
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KLICTTGKLPVPWPTLVTTLGYGLQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYITADKQKNGIK
                     ANFKIRHNIEDGGVQLADHYQQNTPIGDGPVLLPDNHYLSYQSKLSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(77..98)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(338..357)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     685..706
                     /label=EGFP-C
                     /note="EGFP, forward primer"
ORIGIN
        1 gtcagatccg ctagcgctac cggtcgccac catggtgagc aagggcgagg agctgttcac
       61 cggggtggtg cccatcctgg tcgagctgga cggcgacgta aacggccaca agttcagcgt
      121 gtccggcgag ggcgagggcg atgccaccta cggcaagctg accctgaagc tgatctgcac
      181 caccggcaag ctgcccgtgc cctggcccac cctcgtgacc accctgggct acggcctgca
      241 gtgcttcgcc cgctaccccg accacatgaa gcagcacgac ttcttcaagt ccgccatgcc
      301 cgaaggctac gtccaggagc gcaccatctt cttcaaggac gacggcaact acaagacccg
      361 cgccgaggtg aagttcgagg gcgacaccct ggtgaaccgc atcgagctga agggcatcga
      421 cttcaaggag gacggcaaca tcctggggca caagctggag tacaactaca acagccacaa
      481 cgtctatatc accgccgaca agcagaagaa cggcatcaag gccaacttca agatccgcca
      541 caacatcgag gacggcggcg tgcagctcgc cgaccactac cagcagaaca cccccatcgg
      601 cgacggcccc gtgctgctgc ccgacaacca ctacctgagc taccagtcca agctgagcaa
      661 agaccccaac gagaagcgcg atcacatggt cctgctggag ttcgtgaccg ccgccgggat
      721 cactctcggc atggacgagc tgtacaagtc cggactcaga tctatgagcg agacggtcat
      781 ctgttccagc cgggctactg tgatgcttta tgatgacagc aacaagcgat ggctccctgc
      841 tggcactggt ccccaggcct tcagccgcgt ccagatatac cacaacccca ctgctaactc
      901 cttccgagtt gttggccgca agatgcagcc ggatcagcag gtggttatca actgtgccat
      961 cattcggggt gtcaagtaca atcaggccac tcccatcttc catcagtggc gagatgcccg
     1021 ccaggtctgg ggcctcaact tcggcagcaa ggaggacgcc atacagtttg caacaggcat
     1081 ggccaacgcc ctagaggcct tggaaggagg tgggcctccc ccagccccag caccccctgc
     1141 ctggtctgcc cagaatggtc cctccccaga ggagctggaa caacagaaaa ggcagccgga
     1201 gcatatggag cgccgggtct ccaatgcagg aggcccacct gctcccccag ctgggggccc
     1261 tcctccacct ccaggacctc cccctcctcc aggtcccccc ccacccccag gtctgccctc
     1321 ctcaggggta tctggggcag gtcatggagc aggggcagcc ccaccccctg cacccccact
     1381 ccctacaaca cagggcccca atagtggggg ttccggggcc ccaggcctgg ctgctgccat
     1441 tgctggagcc aaactcagga aagtgagcaa gcaggaggag gcctctgggg ggcccctggc
     1501 ccccaaagct gagaacagtc gaagcactgg tggggggctt atggaagaga tgaacgccat
     1561 gctggcccgg agaagaaaag ccacacaggt tggggagaag ccccccaaag acgagtcagc
     1621 cagtgaggag tcagaggccc gactccctgc ccagagtgaa cctgtgagaa gaccctggga
     1681 gaagaacagc acaaccttgc caaggatgaa gtcgtcctct tctgtgacta cctccgaggc
     1741 ccacccctcg acgccctgct ccagtgatga ctccgacttg gagagggtga agcaggagct
     1801 tctggaagag gtgcggaagg agctacagaa aatgaaagag gaaatcatcg aagtctttgt
     1861 ccaggagctg aggaagcggg gttctccttg agcggccgcg actctagatc ataatca
//
