LOCUS       sequence-87514-e        2554 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Focal Adhesions, Excitation: 515, Emission: 528.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2554)
  TITLE     Davidson Yellow fluorescent proteins
REFERENCE   2  (bases 1 to 2554)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: mVenus-Zyxin-6
FEATURES             Location/Qualifiers
     source          1..2554
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      58..67
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     regulatory      1788..1797
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(1794..1796,1797..1799,1800..2513)
                     /codon_start=1
                     /product="Venus YFP with monomerizing A206K mutation (Nagai
                     et al., 2002; Kremers et al., 2006)"
                     /label=mVenus
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KLICTTGKLPVPWPTLVTTLGYGLQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYITADKQKNGIK
                     ANFKIRHNIEDGGVQLADHYQQNTPIGDGPVLLPDNHYLSYQSKLSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(1839..1860)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(2100..2119)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     2447..2468
                     /label=EGFP-C
                     /note="EGFP, forward primer"
ORIGIN
        1 tgaccgtcag atccgctagc gctaccggac tcagatctcg agctcaagct tcgaattcca
       61 tggcggcccc ccgcccgtct cccgcgatcc ccgtttcggt ctcggctccg gctttttacg
      121 ccccgcagaa gaagttcggc cctgtggtgg ccccaaagcc caaagtgaat cccttccggc
      181 ccagggacag cgagcctccc ccggcacccg gggcccagcg cgcacagatg ggccgggtgg
      241 gcgatattcc cccgccgccc ccggaagact ttcccctgcc tccacctccc cttgctgggg
      301 atggcgacga tgcagagggt gctctgggag gtgccttccc gccgccccct cccccgatcg
      361 aggaatcatt tccccctgcg cctctggagg aggagatctt cccttccccg ccgcctcctc
      421 cggaggagga gggagggcct gaggccccca taccgccccc accacagccc agggagaagg
      481 tgagcagtat tgatttggag atcgactctc tgtcctcact gctggatgac atgaccaaga
      541 atgatccttt caaagcccgg gtgtcatctg gatatgtgcc cccaccagtg gccactccat
      601 tcagttccaa gtccagtacc aagcctgcag ccgggggcac agcacccctg cctccttgga
      661 agtccccttc cagctcccag cctctgcccc aggttccggc tccggctcag agccagacac
      721 agttccatgt tcagccccag ccccagccca agcctcaggt ccaactccat gtccagtccc
      781 agacccagcc tgtgtctttg gctaacaccc agccccgagg gcccccagcc tcatctccgg
      841 ctccagcccc taagttttct ccagtgactc ctaagtttac tcctgtggct tccaagttca
      901 gtcctggagc cccaggtgga tctgggtcac aaccaaatca aaaattgggg caccccgaag
      961 ctctttctgc tggcacaggc tcccctcaac ctcccagctt cacctatgcc cagcagaggg
     1021 agaagccccg agtgcaggag aagcagcacc ccgtgccccc accggctcag aaccaaaacc
     1081 aggtgcgctc ccctggggcc ccagggcccc tgactctgaa ggaggtggag gagctggagc
     1141 agctgaccca gcagctaatg caggacatgg agcatcctca gaggcagaat gtggctgtca
     1201 acgaactctg cggccgatgc catcaacccc tggcccgggc gcagccagcc gtccgcgctc
     1261 tagggcagct gttccacatc gcctgcttca cctgccacca gtgtgcgcag cagctccagg
     1321 gccagcagtt ctacagtctg gagggggcgc cgtactgcga gggctgttac actgacaccc
     1381 tggagaagtg taacacctgc ggggagccca tcactgaccg catgctgagg gccacgggca
     1441 aggcctatca cccgcactgc ttcacctgtg tggtctgcgc ccgccccctg gagggcacct
     1501 ccttcatcgt ggaccaggcc aaccggcccc actgtgtccc cgactaccac aagcagtacg
     1561 ccccgaggtg ctccgtctgc tctgagccca tcatgcctga gcctggccga gatgagactg
     1621 tgcgagtggt cgccctggac aagaacttcc acatgaagtg ttacaagtgt gaggactgcg
     1681 ggaagcccct gtcgattgag gcagatgaca atggctgctt ccccctggac ggtcacgtgc
     1741 tctgtcggaa gtgccacact gctagagccc agacggatcc accggtcgcc accatggtga
     1801 gcaagggcga ggagctgttc accggggtgg tgcccatcct ggtcgagctg gacggcgacg
     1861 taaacggcca caagttcagc gtgtccggcg agggcgaggg cgatgccacc tacggcaagc
     1921 tgaccctgaa gctgatctgc accaccggca agctgcccgt gccctggccc accctcgtga
     1981 ccaccctggg ctacggcctg cagtgcttcg cccgctaccc cgaccacatg aagcagcacg
     2041 acttcttcaa gtccgccatg cccgaaggct acgtccagga gcgcaccatc ttcttcaagg
     2101 acgacggcaa ctacaagacc cgcgccgagg tgaagttcga gggcgacacc ctggtgaacc
     2161 gcatcgagct gaagggcatc gacttcaagg aggacggcaa catcctgggg cacaagctgg
     2221 agtacaacta caacagccac aacgtctata tcaccgccga caagcagaag aacggcatca
     2281 aggccaactt caagatccgc cacaacatcg aggacggcgg cgtgcagctc gccgaccact
     2341 accagcagaa cacccccatc ggcgacggcc ccgtgctgct gcccgacaac cactacctga
     2401 gctaccagtc caagctgagc aaagacccca acgagaagcg cgatcacatg gtcctgctgg
     2461 agttcgtgac cgccgccggg atcactctcg gcatggacga gctgtacaag taaagcggcc
     2521 gcgactctag atcataatca gccataccac attt
//
