LOCUS       sequence-87515-e        1932 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Actin, Excitation: 513, Emission: 527.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1932)
  TITLE     Davidson Yellow fluorescent proteins
REFERENCE   2  (bases 1 to 1932)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: YPet-Actin-3
FEATURES             Location/Qualifiers
     source          1..1932
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             join(31..33,34..36,37..747)
                     /codon_start=1
                     /product="yellow fluorescent protein optimized for FRET 
                     with CyPet (Nguyen and Daugherty, 2005)"
                     /label=YPet
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KLLCTTGKLPVPWPTLVTTLGYGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYITADKQKNGIK
                     ANFKIRHNIEDGGVQLADHYQQNTPIGDGPVLLPDNHYLSYQSALFKDPNEKRDHMVLL
                     EFLTAAGITEGMNELYK"
     primer_bind     complement(337..356)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
ORIGIN
        1 gacgtcagat ccgctagcgc taccggtacc atggtgagca aaggcgaaga gctgttcacc
       61 ggcgtggtgc ccatcctggt ggagctggac ggcgacgtga acggccacaa gttcagcgtg
      121 agcggcgagg gcgagggcga cgccacctac ggcaagctga ccctgaagct gctgtgcacc
      181 accggcaagc tgcccgtgcc ctggcccacc ctggtgacca ccctgggcta cggcgtgcag
      241 tgcttcgccc ggtaccccga ccacatgaag cagcacgact tcttcaagag cgccatgccc
      301 gagggctacg tgcaggagcg gaccatcttc ttcaaggacg acggcaacta caagacccgg
      361 gccgaggtga agttcgaggg cgacaccctg gtgaaccgga tcgagctgaa gggcatcgac
      421 ttcaaggagg acggcaacat cctgggccac aagctggagt acaactacaa cagccacaac
      481 gtgtacatca ccgccgacaa gcagaagaac ggcatcaagg ccaacttcaa gatccggcac
      541 aacatcgagg acggcggcgt gcagctggcc gaccactacc agcagaacac ccccatcggc
      601 gacggccccg tgctgctgcc cgacaaccac tacctgagct accagagcgc cctgttcaag
      661 gaccccaacg agaagcggga ccacatggtg ctgctggagt tcctgaccgc cgccggcatc
      721 accgagggca tgaacgagct ctataagtct cgagctatgg atgatgatat cgccgcgctc
      781 gtcgtcgaca acggctccgg catgtgcaag gccggcttcg cgggcgacga tgccccccgg
      841 gccgtcttcc cctccatcgt ggggcgcccc aggcaccagg gcgtgatggt gggcatgggt
      901 cagaaggatt cctatgtggg cgacgaggcc cagagcaaga gaggcatcct caccctgaag
      961 taccccatcg agcacggcat cgtcaccaac tgggacgaca tggagaaaat ctggcaccac
     1021 accttctaca atgagctgcg tgtggctccc gaggagcacc ccgtgctgct gaccgaggcc
     1081 cccctgaacc ccaaggccaa ccgcgagaag atgacccaga tcatgtttga gaccttcaac
     1141 accccagcca tgtacgttgc tatccaggct gtgctatccc tgtacgcctc tggccgtacc
     1201 actggcatcg tgatggactc cggtgacggg gtcacccaca ctgtgcccat ctacgagggg
     1261 tatgccctcc cccatgccat cctgcgtctg gacctggctg gccgggacct gactgactac
     1321 ctcatgaaga tcctcaccga gcgcggctac agcttcacca ccacggccga gcgggaaatc
     1381 gtgcgtgaca ttaaggagaa gctgtgctac gtcgccctgg acttcgagca agagatggcc
     1441 acggctgctt ccagctcctc cctggagaag agctacgagc tgcctgacgg ccaggtcatc
     1501 accattggca atgagcggtt ccgctgccct gaggcactct tccagccttc cttcctgggc
     1561 atggagtcct gtggcatcca cgaaactacc ttcaactcca tcatgaagtg tgacgtggac
     1621 atccgcaaag acctgtacgc caacacagtg ctgtctggcg gcaccaccat gtaccctggc
     1681 attgccgaca ggatgcagaa ggagatcact gccctggcac ccagcacaat gaagatcaag
     1741 atcattgctc ctcctgagcg caagtactcc gtgtggatcg gcggctccat cctggcctcg
     1801 ctgtccacct tccagcagat gtggatcagc aagcaggagt atgacgagtc cggcccctcc
     1861 atcgtccacc gcaaatgctt ctagggatcc accggatcta gataactgac aaatcaccat
     1921 accacatgta ga
//
