LOCUS       sequence-87516-e         983 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Actin, Excitation: 513, Emission: 527.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 983)
  TITLE     Davidson Yellow fluorescent proteins
REFERENCE   2  (bases 1 to 983)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: YPet-Actin-7
FEATURES             Location/Qualifiers
     source          1..983
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      27..36
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(33..35,36..38,39..749)
                     /codon_start=1
                     /product="yellow fluorescent protein optimized for FRET 
                     with CyPet (Nguyen and Daugherty, 2005)"
                     /label=YPet
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KLLCTTGKLPVPWPTLVTTLGYGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYITADKQKNGIK
                     ANFKIRHNIEDGGVQLADHYQQNTPIGDGPVLLPDNHYLSYQSALFKDPNEKRDHMVLL
                     EFLTAAGITEGMNELYK"
     primer_bind     complement(339..358)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
ORIGIN
        1 cgtcagatcc gctagcgcta ccggtcgcca ccatggtgag caaaggcgaa gagctgttca
       61 ccggcgtggt gcccatcctg gtggagctgg acggcgacgt gaacggccac aagttcagcg
      121 tgagcggcga gggcgagggc gacgccacct acggcaagct gaccctgaag ctgctgtgca
      181 ccaccggcaa gctgcccgtg ccctggccca ccctggtgac caccctgggc tacggcgtgc
      241 agtgcttcgc ccggtacccc gaccacatga agcagcacga cttcttcaag agcgccatgc
      301 ccgagggcta cgtgcaggag cggaccatct tcttcaagga cgacggcaac tacaagaccc
      361 gggccgaggt gaagttcgag ggcgacaccc tggtgaaccg gatcgagctg aagggcatcg
      421 acttcaagga ggacggcaac atcctgggcc acaagctgga gtacaactac aacagccaca
      481 acgtgtacat caccgccgac aagcagaaga acggcatcaa ggccaacttc aagatccggc
      541 acaacatcga ggacggcggc gtgcagctgg ccgaccacta ccagcagaac acccccatcg
      601 gcgacggccc cgtgctgctg cccgacaacc actacctgag ctaccagagc gccctgttca
      661 aggaccccaa cgagaagcgg gaccacatgg tgctgctgga gttcctgacc gccgccggca
      721 tcaccgaggg catgaacgag ctctataagt ccggactcag atctcgagct atggatgatg
      781 atatcgccgc gctcgtcgtc gacaacggct ccggcatgtg caaggccggc ttcgcgggcg
      841 acgatgcccc ccgggccgtc ttcccctcca tcgtggggcg ccccaggcac cagggcgtga
      901 tggtgggcat gggtcagaag gattcctatg tgggcgacga ggcccagagc aagagaggca
      961 tcctcaccct gaagtacccc atc
//
