LOCUS       sequence-87518-e        1566 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Gap Junctions, Excitation: 513, Emission: 527.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1566)
  TITLE     Davidson Yellow fluorescent proteins
REFERENCE   2  (bases 1 to 1566)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: YPet-Cx26-7
FEATURES             Location/Qualifiers
     source          1..1566
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      798..807
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(804..806,807..809,810..1523)
                     /codon_start=1
                     /product="yellow fluorescent protein optimized for FRET 
                     with CyPet (Nguyen and Daugherty, 2005)"
                     /label=YPet
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KLLCTTGKLPVPWPTLVTTLGYGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYITADKQKNGIK
                     ANFKIRHNIEDGGVQLADHYQQNTPIGDGPVLLPDNHYLSYQSALFKDPNEKRDHMVLL
                     EFLTAAGITEGMNELYK"
     primer_bind     complement(1110..1129)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
ORIGIN
        1 gacgtcagat ccgctagcgc taccggactc agatctcgag ctcaagcttc gaattcgcgg
       61 ccgcacgctc ctcgggacac agtgccaacc atccagagga caagatggat tggggcacac
      121 tacagagcat cctcgggggt gtcaacaagc actccaccag cattgggaaa atctggctca
      181 ctgtcctctt catcttccgc atcatgatcc tcgtggtggc cgcgaaggag gtgtggggag
      241 atgagcaagc cgattttgtt tgcaacactc tccagcctgg ctgtaagaat gtgtgctacg
      301 accactactt ccccatctct cacatccggc tctgggctct gcagctgatc atggtgtcca
      361 cgccggccct cctggtagct atgcacgtgg cctaccggag acacgaaaag aaacggaagt
      421 tcatgaaggg agagataaag aacgagttta aggacatcga agagatcaaa acccagaagg
      481 tccgtatcga agggtccctg tggtggacct acaccaccag catcttcttc cgggtcatct
      541 tcgaagctgt cttcatgtat gtcttttaca tcatgtacaa tggcttcttc atgcagcgtc
      601 tggtgaagtg taacgcctgg ccttgtccca atacagtgga ctgcttcatt tccaggccca
      661 cagaaaagac tgtcttcacg gtgttcatga tctctgtgtc tggaatttgc atcctgctaa
      721 acatcacaga gctgtgctat ctgttcatta ggtattgctc agggaagtcc aaaagaccag
      781 tcgcggatcc accggtcgcc accatggtga gcaaaggcga agagctgttc accggcgtgg
      841 tgcccatcct ggtggagctg gacggcgacg tgaacggcca caagttcagc gtgagcggcg
      901 agggcgaggg cgacgccacc tacggcaagc tgaccctgaa gctgctgtgc accaccggca
      961 agctgcccgt gccctggccc accctggtga ccaccctggg ctacggcgtg cagtgcttcg
     1021 cccggtaccc cgaccacatg aagcagcacg acttcttcaa gagcgccatg cccgagggct
     1081 acgtgcagga gcggaccatc ttcttcaagg acgacggcaa ctacaagacc cgggccgagg
     1141 tgaagttcga gggcgacacc ctggtgaacc ggatcgagct gaagggcatc gacttcaagg
     1201 aggacggcaa catcctgggc cacaagctgg agtacaacta caacagccac aacgtgtaca
     1261 tcaccgccga caagcagaag aacggcatca aggccaactt caagatccgg cacaacatcg
     1321 aggacggcgg cgtgcagctg gccgaccact accagcagaa cacccccatc ggcgacggcc
     1381 ccgtgctgct gcccgacaac cactacctga gctaccagag cgccctgttc aaggacccca
     1441 acgagaagcg ggaccacatg gtgctgctgg agttcctgac cgccgccggc atcaccgagg
     1501 gcatgaacga gctctataag taaagcggcc gcgactctag atcataatca gccataccac
     1561 attgag
//
