LOCUS       sequence-87520-e        2164 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Gap Junctions, Excitation: 513, Emission: 527.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2164)
  TITLE     Davidson Yellow fluorescent proteins
REFERENCE   2  (bases 1 to 2164)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: YPet-Cx43-7
FEATURES             Location/Qualifiers
     source          1..2164
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      1399..1408
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(1405..1407,1408..1410,1411..2124)
                     /codon_start=1
                     /product="yellow fluorescent protein optimized for FRET 
                     with CyPet (Nguyen and Daugherty, 2005)"
                     /label=YPet
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KLLCTTGKLPVPWPTLVTTLGYGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYITADKQKNGIK
                     ANFKIRHNIEDGGVQLADHYQQNTPIGDGPVLLPDNHYLSYQSALFKDPNEKRDHMVLL
                     EFLTAAGITEGMNELYK"
     primer_bind     complement(1711..1730)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
ORIGIN
        1 atccgctagc gctaccggac tcagatctcg agctcaagct tcgaattcgg cagcactttt
       61 ctttcattgg ggggaaggcg tgaggaaagt accaaacagc agcagacttt taaactttaa
      121 acagacaggt ctgagagcct gaactctcat ttttcctttg acttcagcct ccaaggagtt
      181 ccaccaactt tggcgcgccg gcttcacttt cattaagtga aagagaggtg cccagacatg
      241 ggtgactgga gtgccttggg gaagcttctg gacaaggtcc aagcctactc caccgctgga
      301 gggaaggtgt ggctgtcagt gctcttcata ttcagaatcc tgctcctggg gacagctgtt
      361 gagtcagctt ggggtgatga acagtctgcc tttcgctgta acactcaaca acctggctgc
      421 gaaaacgtct gctatgacaa gtccttcccc atctctcacg tgcgcttctg ggtccttcag
      481 atcatattcg tgtctgtgcc cacactcctg tacttggccc atgtgttcta tgtgatgagg
      541 aaggaagaga agctaaacaa gaaagaagag gagctcaaag tggcccagac tgacggggtc
      601 aacgtggaga tgcacctgaa gcagattgaa atcaagaagt tcaagtacgg gattgaagag
      661 cacggcaagg tgaaaatgag gggcggcttg ctgagaacct acatcatcag catcctcttc
      721 aagtctgtct tcgaggtggc cttcctgctc atccagtggt acatctatgg gttcagcttg
      781 agcgcggtct acacctgcaa gagagatccc tgcccccacc aggtagactg cttcctctca
      841 cgtcccacgg agaaaaccat cttcatcatc ttcatgctgg tggtgtcctt ggtgtctctc
      901 gctttgaaca tcattgagct cttctacgtc ttcttcaaag gcgttaagga tcgcgtgaag
      961 ggaagaagcg atccttacca cgccaccact ggcccactga gcccatcaaa agactgcgga
     1021 tctccaaaat acgcctactt caatggctgc tcctcaccaa cggctccact ctcgcctatg
     1081 tctcctcctg ggtacaagct ggttactggt gacagaaaca attcctcgtg ccgcaattac
     1141 aacaagcaag ctagcgagca aaactgggcg aactacagcg cagagcaaaa tcgcatgggg
     1201 caggccggaa gcaccatctc caactcgcac gcccagccgt tcgatttccc cgacgacaac
     1261 cagaatgcca aaaaagttgc tgctggacat gaactccagc cattagccat cgtggaccaa
     1321 cgaccttcca gcagagccag cagccgcgcc agcagcaggc ctcggcctga tgacctggag
     1381 attgcggatc caccggtcgc caccatggtg agcaaaggcg aagagctgtt caccggcgtg
     1441 gtgcccatcc tggtggagct ggacggcgac gtgaacggcc acaagttcag cgtgagcggc
     1501 gagggcgagg gcgacgccac ctacggcaag ctgaccctga agctgctgtg caccaccggc
     1561 aagctgcccg tgccctggcc caccctggtg accaccctgg gctacggcgt gcagtgcttc
     1621 gcccggtacc ccgaccacat gaagcagcac gacttcttca agagcgccat gcccgagggc
     1681 tacgtgcagg agcggaccat cttcttcaag gacgacggca actacaagac ccgggccgag
     1741 gtgaagttcg agggcgacac cctggtgaac cggatcgagc tgaagggcat cgacttcaag
     1801 gaggacggca acatcctggg ccacaagctg gagtacaact acaacagcca caacgtgtac
     1861 atcaccgccg acaagcagaa gaacggcatc aaggccaact tcaagatccg gcacaacatc
     1921 gaggacggcg gcgtgcagct ggccgaccac taccagcaga acacccccat cggcgacggc
     1981 cccgtgctgc tgcccgacaa ccactacctg agctaccaga gcgccctgtt caaggacccc
     2041 aacgagaagc gggaccacat ggtgctgctg gagttcctga ccgccgccgg catcaccgag
     2101 ggcatgaacg agctctataa gtaaagcggc cgcgactcta gatcataatc agccatacca
     2161 catt
//
