LOCUS       sequence-87521-e        1239 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Histone H2B, Excitation: 513, Emission: 527.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1239)
  TITLE     Davidson Yellow fluorescent proteins
REFERENCE   2  (bases 1 to 1239)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: YPet-H2B-6
FEATURES             Location/Qualifiers
     source          1..1239
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             78..455
                     /codon_start=1
                     /gene="HIST1H2BJ"
                     /product="human histone H2B"
                     /label=H2B
                     /translation="MPEPAKSAPAPKKGSKKAVTKAQKKGGKKRKRSRKESYSIYVYKV
                     LKQVHPDTGISSKAMGIMNSFVNDIFERIAGEASRLAHYNKRSTITSREIQTAVRLLLP
                     GELAKHAVSEGTKAITKYTSAK"
     regulatory      468..477
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(474..476,477..479,480..1193)
                     /codon_start=1
                     /product="yellow fluorescent protein optimized for FRET 
                     with CyPet (Nguyen and Daugherty, 2005)"
                     /label=YPet
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KLLCTTGKLPVPWPTLVTTLGYGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYITADKQKNGIK
                     ANFKIRHNIEDGGVQLADHYQQNTPIGDGPVLLPDNHYLSYQSALFKDPNEKRDHMVLL
                     EFLTAAGITEGMNELYK"
     primer_bind     complement(780..799)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
ORIGIN
        1 acgtcagatc cgctagcgct accggactca gatctcgagc tcaagcttcg aattctgcag
       61 tcgacggtac cgccaccatg ccagagccag cgaagtctgc tcccgccccg aaaaagggct
      121 ccaagaaggc ggtgactaag gcgcagaaga aaggcggcaa gaagcgcaag cgcagccgca
      181 aggagagcta ttccatctat gtgtacaagg ttctgaagca ggtccaccct gacaccggca
      241 tttcgtccaa ggccatgggc atcatgaatt cgtttgtgaa cgacattttc gagcgcatcg
      301 caggtgaggc ttcccgcctg gcgcattaca acaagcgctc gaccatcacc tccagggaga
      361 tccagacggc cgtgcgcctg ctgctgcctg gggagttggc caagcacgcc gtgtccgagg
      421 gtactaaggc catcaccaag tacaccagcg ctaaggatcc accggtcgcc accatggtga
      481 gcaaaggcga agagctgttc accggcgtgg tgcccatcct ggtggagctg gacggcgacg
      541 tgaacggcca caagttcagc gtgagcggcg agggcgaggg cgacgccacc tacggcaagc
      601 tgaccctgaa gctgctgtgc accaccggca agctgcccgt gccctggccc accctggtga
      661 ccaccctggg ctacggcgtg cagtgcttcg cccggtaccc cgaccacatg aagcagcacg
      721 acttcttcaa gagcgccatg cccgagggct acgtgcagga gcggaccatc ttcttcaagg
      781 acgacggcaa ctacaagacc cgggccgagg tgaagttcga gggcgacacc ctggtgaacc
      841 ggatcgagct gaagggcatc gacttcaagg aggacggcaa catcctgggc cacaagctgg
      901 agtacaacta caacagccac aacgtgtaca tcaccgccga caagcagaag aacggcatca
      961 aggccaactt caagatccgg cacaacatcg aggacggcgg cgtgcagctg gccgaccact
     1021 accagcagaa cacccccatc ggcgacggcc ccgtgctgct gcccgacaac cactacctga
     1081 gctaccagag cgccctgttc aaggacccca acgagaagcg ggaccacatg gtgctgctgg
     1141 agttcctgac cgccgccggc atcaccgagg gcatgaacga gctctataag taaagcggcc
     1201 gcgactctag atcataatca gccataccac attgagagg
//
