LOCUS       sequence-87522-e        1050 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Golgi, Excitation: 513, Emission: 527.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1050)
  TITLE     Davidson Yellow fluorescent proteins
REFERENCE   2  (bases 1 to 1050)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: YPet-Golgi-7
FEATURES             Location/Qualifiers
     source          1..1050
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      281..290
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(287..289,290..292,293..1006)
                     /codon_start=1
                     /product="yellow fluorescent protein optimized for FRET 
                     with CyPet (Nguyen and Daugherty, 2005)"
                     /label=YPet
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KLLCTTGKLPVPWPTLVTTLGYGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYITADKQKNGIK
                     ANFKIRHNIEDGGVQLADHYQQNTPIGDGPVLLPDNHYLSYQSALFKDPNEKRDHMVLL
                     EFLTAAGITEGMNELYK"
     primer_bind     complement(593..612)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
ORIGIN
        1 gaacgtcaga tccgctagca tgaggcttcg ggagccgctc ctgagcggca gcgccgcgat
       61 gccaggcgcg tccctacagc gggcctgccg cctgctcgtg gccgtctgcg ctctgcacct
      121 tggcgtcacc ctcgtttact acctggctgg ccgcgacctg agccgcctgc cccaactggt
      181 cggagtctcc acaccgctgc agggcggctc gaacagtgcc gccgccatcg ggcagtcctc
      241 cggggagctc cggaccggag gggccaagga tccaccggtc gccaccatgg tgagcaaagg
      301 cgaagagctg ttcaccggcg tggtgcccat cctggtggag ctggacggcg acgtgaacgg
      361 ccacaagttc agcgtgagcg gcgagggcga gggcgacgcc acctacggca agctgaccct
      421 gaagctgctg tgcaccaccg gcaagctgcc cgtgccctgg cccaccctgg tgaccaccct
      481 gggctacggc gtgcagtgct tcgcccggta ccccgaccac atgaagcagc acgacttctt
      541 caagagcgcc atgcccgagg gctacgtgca ggagcggacc atcttcttca aggacgacgg
      601 caactacaag acccgggccg aggtgaagtt cgagggcgac accctggtga accggatcga
      661 gctgaagggc atcgacttca aggaggacgg caacatcctg ggccacaagc tggagtacaa
      721 ctacaacagc cacaacgtgt acatcaccgc cgacaagcag aagaacggca tcaaggccaa
      781 cttcaagatc cggcacaaca tcgaggacgg cggcgtgcag ctggccgacc actaccagca
      841 gaacaccccc atcggcgacg gccccgtgct gctgcccgac aaccactacc tgagctacca
      901 gagcgccctg ttcaaggacc ccaacgagaa gcgggaccac atggtgctgc tggagttcct
      961 gaccgccgcc ggcatcaccg agggcatgaa cgagctctat aagtaaagcg gccgcgactc
     1021 tagatcataa tcagccatac cacattgtag
//
