LOCUS       sequence-87525-e        2088 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Lysosome Membrane, Excitation: 513, Emission: 527.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2088)
  TITLE     Davidson Yellow fluorescent proteins
REFERENCE   2  (bases 1 to 2088)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: YPet-Lysosomes-20
FEATURES             Location/Qualifiers
     source          1..2088
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      42..51
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     regulatory      46..55
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(48..110,111..1268)
                     /codon_start=1
                     /product="rat lysosome-associated membrane glycoprotein 1"
                     /label=LAMP1
                     /translation="MAAPGARRPLLLLLLAGLAHSAPALFEVKDNNGTACIMASFSASF
                     LTTYEAGHVSKVSNMTLPASAEVLKNSSSCGEKNASEPTLAITFGEGYLLKLTFTKNTT
                     RYSVQHMYFTYNLSDTQFFPNASSKGPDTVDSTTDIKADINKTYRCVSDIRVYMKNVTI
                     VLWDATIQAYLPSSNFSKEETRCPQDQPSPTTGPPSPSPPLVPTNPSVSKYNVTGDNGT
                     CLLASMALQLNITYMKKDNTTVTRAFNINPSDKYSGTCGAQLVTLKVGNKSRVLELQFG
                     MNATSSLFFLQGVQLNMTLPDAIEPTFSTSNYSLKALQASVGNSYKCNSEEHIFVSKAL
                     ALNVFSVQVQAFRVESDRFGSVEECVQDGNNMLIPIAVGGALAGLVLIVLIAYLIGRKR
                     SHAGYQTI"
     regulatory      1323..1332
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(1329..1331,1332..1334,1335..2048)
                     /codon_start=1
                     /product="yellow fluorescent protein optimized for FRET 
                     with CyPet (Nguyen and Daugherty, 2005)"
                     /label=YPet
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KLLCTTGKLPVPWPTLVTTLGYGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYITADKQKNGIK
                     ANFKIRHNIEDGGVQLADHYQQNTPIGDGPVLLPDNHYLSYQSALFKDPNEKRDHMVLL
                     EFLTAAGITEGMNELYK"
     primer_bind     complement(1635..1654)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
ORIGIN
        1 gacgtcagat ccgctagcgc taccggactc agatctcgag cgccaccatg gcggccccgg
       61 gcgcccggcg gccgctgctc ctgttgctgc tggcaggcct tgcacacagc gccccagcac
      121 tgttcgaggt gaaagacaac aacggcacag cgtgtataat ggccagcttc tctgcctcct
      181 ttctgaccac ctatgaggct ggacatgttt ctaaggtctc gaatatgacc ctgccagcct
      241 ctgcagaagt cctgaagaat agcagctctt gtggtgaaaa gaatgcttct gagcccaccc
      301 tcgcaatcac ctttggagaa ggatatttac tgaaactcac cttcacaaaa aacacaacac
      361 gttacagtgt ccagcacatg tatttcacat ataacctgtc agacacacaa ttctttccca
      421 atgccagctc caaagggccc gacactgtgg attccacaac tgacatcaag gcagacatca
      481 acaaaacata ccgatgtgtc agcgacatca gggtctacat gaagaatgtg accattgtgc
      541 tctgggacgc tactatccag gcctacctgc cgagtagcaa cttcagcaag gaagagacac
      601 gctgcccaca ggatcaacct tccccaacta ctgggccacc cagcccctca ccaccacttg
      661 tgcccacaaa ccccagtgtg tccaagtaca atgtgactgg tgacaatgga acctgcctgc
      721 tggcctctat ggcactgcaa ctcaacatca cctacatgaa gaaggacaac acgactgtga
      781 ccagagcatt caacatcaac ccaagtgaca aatatagtgg gacttgcggt gcccagttgg
      841 tgaccctgaa ggtggggaac aagagcagag tcctggagct gcagtttggg atgaatgcca
      901 cttctagcct gtttttcctg caaggagttc agttgaacat gactcttcct gatgccatag
      961 agcccacgtt cagcacctcc aactattccc tgaaagctct tcaggccagt gtcggcaact
     1021 catacaagtg caacagtgag gagcacatct ttgtcagcaa ggcgctcgcc ctcaatgtct
     1081 tcagcgtgca agtccaggct ttcagggtag aaagtgacag gtttgggtct gtggaagagt
     1141 gtgtacagga cggtaacaac atgctgatcc ccattgctgt gggcggggcc ctggcagggc
     1201 tggtcctcat cgtcctcatc gcctacctca tcggcaggaa gaggagtcac gcgggctatc
     1261 agaccatctc ggaattcggc tccaccggct ccaccggctc caccggcgcg gatccaccgg
     1321 tcgccaccat ggtgagcaaa ggcgaagagc tgttcaccgg cgtggtgccc atcctggtgg
     1381 agctggacgg cgacgtgaac ggccacaagt tcagcgtgag cggcgagggc gagggcgacg
     1441 ccacctacgg caagctgacc ctgaagctgc tgtgcaccac cggcaagctg cccgtgccct
     1501 ggcccaccct ggtgaccacc ctgggctacg gcgtgcagtg cttcgcccgg taccccgacc
     1561 acatgaagca gcacgacttc ttcaagagcg ccatgcccga gggctacgtg caggagcgga
     1621 ccatcttctt caaggacgac ggcaactaca agacccgggc cgaggtgaag ttcgagggcg
     1681 acaccctggt gaaccggatc gagctgaagg gcatcgactt caaggaggac ggcaacatcc
     1741 tgggccacaa gctggagtac aactacaaca gccacaacgt gtacatcacc gccgacaagc
     1801 agaagaacgg catcaaggcc aacttcaaga tccggcacaa catcgaggac ggcggcgtgc
     1861 agctggccga ccactaccag cagaacaccc ccatcggcga cggccccgtg ctgctgcccg
     1921 acaaccacta cctgagctac cagagcgccc tgttcaagga ccccaacgag aagcgggacc
     1981 acatggtgct gctggagttc ctgaccgccg ccggcatcac cgagggcatg aacgagctct
     2041 ataagtaaag cggccgcgac tctagatcat aatcagccat accacatt
//
