LOCUS       sequence-87526-e        1026 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Mitochondria, Excitation: 513, Emission: 527.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1026)
  TITLE     Davidson Yellow fluorescent proteins
REFERENCE   2  (bases 1 to 1026)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: YPet-Mito-7
FEATURES             Location/Qualifiers
     source          1..1026
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             86..160
                     /codon_start=1
                     /product="mitochondrial presequence of human cytochrome c 
                     oxidase subunit VIII (Rizutto et al., 1989)"
                     /label=COX8 presequence
                     /translation="MSVLTPLLLRGLTGSARRLPVPRAK"
     regulatory      188..197
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(194..196,197..199,200..913)
                     /codon_start=1
                     /product="yellow fluorescent protein optimized for FRET 
                     with CyPet (Nguyen and Daugherty, 2005)"
                     /label=YPet
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KLLCTTGKLPVPWPTLVTTLGYGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYITADKQKNGIK
                     ANFKIRHNIEDGGVQLADHYQQNTPIGDGPVLLPDNHYLSYQSALFKDPNEKRDHMVLL
                     EFLTAAGITEGMNELYK"
     primer_bind     complement(500..519)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
ORIGIN
        1 tccgccccat gacgcaaatg gcggtagcgt gtacgtggga ggtctatata gcagagctgg
       61 ttagtgaacc gtcagatccg ctagcatgtc cgtcctgacg ccgctgctgc tgcggggctt
      121 gacaggctcg gcccggcggc tcccagtgcc gcgcgccaag atccattcgt tgggggatcc
      181 accggtcgcc accatggtga gcaaaggcga agagctgttc accggcgtgg tgcccatcct
      241 ggtggagctg gacggcgacg tgaacggcca caagttcagc gtgagcggcg agggcgaggg
      301 cgacgccacc tacggcaagc tgaccctgaa gctgctgtgc accaccggca agctgcccgt
      361 gccctggccc accctggtga ccaccctggg ctacggcgtg cagtgcttcg cccggtaccc
      421 cgaccacatg aagcagcacg acttcttcaa gagcgccatg cccgagggct acgtgcagga
      481 gcggaccatc ttcttcaagg acgacggcaa ctacaagacc cgggccgagg tgaagttcga
      541 gggcgacacc ctggtgaacc ggatcgagct gaagggcatc gacttcaagg aggacggcaa
      601 catcctgggc cacaagctgg agtacaacta caacagccac aacgtgtaca tcaccgccga
      661 caagcagaag aacggcatca aggccaactt caagatccgg cacaacatcg aggacggcgg
      721 cgtgcagctg gccgaccact accagcagaa cacccccatc ggcgacggcc ccgtgctgct
      781 gcccgacaac cactacctga gctaccagag cgccctgttc aaggacccca acgagaagcg
      841 ggaccacatg gtgctgctgg agttcctgac cgccgccggc atcaccgagg gcatgaacga
      901 gctctataag taaagcggcc gcgactctag atcatatcag catacacatt tgtagaggtt
      961 tactgcttta aaacctccac acctcccctg actgaacata aatgatgcat gtgtgtactg
     1021 tatgca
//
